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| This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. This mutant mouse strain represents a model that may be useful in studies of skeletal development. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 31-DEC-08 Species laboratory mouse Generation N10F3 (11-APR-11)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector containing nuclear localized cre coding sequence, loxP site flanked neomycin resistance cassette and a diphtheria toxin gene was inserted 90 bp upstream of the initiation codon to disrupt the targeted gene. The endogenous Myf5 promoter drives expression of the Cre recombinase. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Cre recombinase expressing strain to remove the neomycin selection cassette and then backcrossed to C57BL/6J mice for at least 5 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Myf5
010529 B6;129-Myf5tm1(cre)Mrc/J 002522 C.129S4-Myf5tm1Jae/J 018626 STOCK Myf5tm1Pas/J View Strains carrying other alleles of Myf5 (3 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Myf5tm3(cre)Sor/Myf5tm3(cre)Sor
involves: 129S4/SvJae * C57BL/6J
- skeleton phenotype
- abnormal rib development
- animals show more rib formation than Myf5tm1Jae homozygotes, but ribs frequently do not extend to the vertebrae (MGI Ref ID J:65301)
- cervical vertebral fusion
- fusions are seen in most animals, but are less severe than in Myf5tm1Jae homozygotes (MGI Ref ID J:65301)
- thoracic vertebral fusion
- fusions are seen in most animals, but are less severe than in Myf5tm1Jae homozygotes (MGI Ref ID J:65301)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Dlk1tm1.1Jvs/Dlk1+ Myf5tm3(cre)Sor/Myf5+
involves: 129P2 * 129S4/SvJaeSor * C57BL/6 * FVB/N (conditional)
- muscle phenotype
- abnormal muscle regeneration
- impaired regeneration at 5-7 days after intramuscular injections of CTX to the tibialis anterior (TA) muscles when the allele is inherited paternally (MGI Ref ID J:167124)
- roughly 25% decrease in nascent de novo fiber formation five days after injury when the allele is inherited paternally (MGI Ref ID J:167124)
- extensive fibrosis, scarification, and interstitial space occupied by massive cellular infiltration when the allele is inherited paternally (MGI Ref ID J:167124)
- decreased skeletal muscle fiber number
- 25% reduction in total myofiber numbers in fast (EDL) and slow (soleus) muscles when the allele is inherited paternally (MGI Ref ID J:167124)
- increased skeletal muscle fiber size
- increased average size of the myotubes formed in satellite cell-derived primary myoblasts over-expressing Dlk1 when the allele is inherited paternally (MGI Ref ID J:167124)
- increased skeletal muscle fiber diameter
- increased diameters of the resulting myotubes in the presence of Dlk1 coated substrate when the allele is inherited paternally (MGI Ref ID J:167124)
- skeletal muscle fibrosis
- extensive fibrosis, scarification, and interstitial space occupied by massive cellular infiltration at 5-7 days after intramuscular injections of CTX to the tibialis anterior (TA) muscles when the allele is inherited paternally (MGI Ref ID J:167124)
- cellular phenotype
- abnormal cell differentiation
- increased proportion of self-renewing cells (Pax7+/MyoD-) in the cultured myoblasts on the mutant EDL fiber when the allele is inherited paternally (MGI Ref ID J:167124)
- accelerated differentiation kinetics in satellite cell-derived primary myoblasts over-expressing Dlk1 or in the presence of Dlk1 coated substrate when the allele is inherited paternally (MGI Ref ID J:167124)
- decreased cell proliferation
- decreased proliferating cells (Pax7+/ MyoD+) in the cultured primary myoblasts on the mutant EDL fiber when the allele is inherited paternally (MGI Ref ID J:167124)
- reduced cell numbers at day 3 after transfection in satellite cell-derived primary myoblasts over-expressing Dlk1 when the allele is inherited paternally (MGI Ref ID J:167124)
- reduced numbers of proliferating cells (Ki67+) in satellite cell-derived primary myoblasts over-expressing Dlk1 or in the presence of Dlk1 coated substrate when the allele is inherited paternally (MGI Ref ID J:167124)
- maternal imprinting
- Dlk1 is an imprinted gene expressed only from paternal allele (MGI Ref ID J:167124)
- growth/size phenotype
- decreased body weight
- reduced body weight when the allele is inherited paternally (MGI Ref ID J:167124)
- adipose tissue phenotype
- decreased brown adipose tissue amount
- decreased brown fat mass by 20% when the allele is inherited paternally (MGI Ref ID J:167124)
- immune system phenotype
- increased inflammatory response
- increased interstitial nuclei density- excessive infiltrated macrophages after injury when the allele is inherited paternally (MGI Ref ID J:167124)
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Perinatal Lethality
Homozygous
Skeletal Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Myf5tm3(cre)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Philippe Soriano | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Mif5-Cre; Myf5Cre; | ||
| Mutation Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | skeletal muscle, dermis, and several ectopic locations | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Myf5 | ||
| Molecular Note | A cre cDNA was inserted between a SacI site and a KpnI site in the 5'UTR of the Myf5 gene. An adjacent loxP-flanked neomycin cassette was removed by Cre- mediated recombination. [MGI Ref ID J:65301] | ||
| Gene Symbol and Name | Myf5, myogenic factor 5 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | B130010J22Rik; Myf-5; RIKEN cDNA B130010J22 gene; bHLHc2; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Myf5tm3(cre)Sor, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Tallquist MD; Weismann KE; Hellstrom M; Soriano P. 2000. Early myotome specification regulates PDGFA expression and axial skeleton development Development 127(23):5059-70. [PubMed: 11060232] [MGI Ref ID J:65301]
Additional References
Myf5tm3(cre)Sor relatedAbou-Khalil R; Le Grand F; Pallafacchina G; Valable S; Authier FJ; Rudnicki MA; Gherardi RK; Germain S; Chretien F; Sotiropoulos A; Lafuste P; Montarras D; Chazaud B. 2009. Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal. Cell Stem Cell 5(3):298-309. [PubMed: 19733541] [MGI Ref ID J:154124]
An CI; Dong Y; Hagiwara N. 2011. Genome-wide mapping of Sox6 binding sites in skeletal muscle reveals both direct and indirect regulation of muscle terminal differentiation by Sox6. BMC Dev Biol 11:59. [PubMed: 21985497] [MGI Ref ID J:178848]
Angione AR; Jiang C; Pan D; Wang YX; Kuang S. 2011. PPARdelta regulates satellite cell proliferation and skeletal muscle regeneration. Skelet Muscle 1(1):33. [PubMed: 22040534] [MGI Ref ID J:183730]
Beedle AM; Turner AJ; Saito Y; Lueck JD; Foltz SJ; Fortunato MJ; Nienaber PM; Campbell KP. 2012. Mouse fukutin deletion impairs dystroglycan processing and recapitulates muscular dystrophy. J Clin Invest 122(9):3330-42. [PubMed: 22922256] [MGI Ref ID J:187144]
Clow C; Jasmin BJ. 2010. Brain-derived neurotrophic factor regulates satellite cell differentiation and skeltal muscle regeneration. Mol Biol Cell 21(13):2182-90. [PubMed: 20427568] [MGI Ref ID J:165062]
Dong F; Sun X; Liu W; Ai D; Klysik E; Lu MF; Hadley J; Antoni L; Chen L; Baldini A; Francis-West P; Martin JF. 2006. Pitx2 promotes development of splanchnic mesoderm-derived branchiomeric muscle. Development 133(24):4891-9. [PubMed: 17107996] [MGI Ref ID J:119660]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Gensch N; Borchardt T; Schneider A; Riethmacher D; Braun T. 2008. Different autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesis. Development 135(9):1597-604. [PubMed: 18367555] [MGI Ref ID J:134484]
Gruenbaum-Cohen Y; Harel I; Umansky KB; Tzahor E; Snapper SB; Shilo BZ; Schejter ED. 2012. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A 109(28):11211-6. [PubMed: 22736793] [MGI Ref ID J:186396]
Hallock PT; Xu CF; Park TJ; Neubert TA; Curran T; Burden SJ. 2010. Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L. Genes Dev 24(21):2451-61. [PubMed: 21041412] [MGI Ref ID J:165470]
Han D; Zhao H; Parada C; Hacia JG; Bringas P Jr; Chai Y. 2012. A TGFbeta-Smad4-Fgf6 signaling cascade controls myogenic differentiation and myoblast fusion during tongue development. Development 139(9):1640-50. [PubMed: 22438570] [MGI Ref ID J:184019]
Harel I; Maezawa Y; Avraham R; Rinon A; Ma HY; Cross JW; Leviatan N; Hegesh J; Roy A; Jacob-Hirsch J; Rechavi G; Carvajal J; Tole S; Kioussi C; Quaggin S; Tzahor E. 2012. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis. Proc Natl Acad Sci U S A 109(46):18839-44. [PubMed: 23112163] [MGI Ref ID J:191738]
Harel I; Nathan E; Tirosh-Finkel L; Zigdon H; Guimaraes-Camboa N; Evans SM; Tzahor E. 2009. Distinct origins and genetic programs of head muscle satellite cells. Dev Cell 16(6):822-32. [PubMed: 19531353] [MGI Ref ID J:150129]
Hatley ME; Tang W; Garcia MR; Finkelstein D; Millay DP; Liu N; Graff J; Galindo RL; Olson EN. 2012. A mouse model of rhabdomyosarcoma originating from the adipocyte lineage. Cancer Cell 22(4):536-46. [PubMed: 23079662] [MGI Ref ID J:192027]
Hosokawa R; Oka K; Yamaza T; Iwata J; Urata M; Xu X; Bringas P Jr; Nonaka K; Chai Y. 2010. TGF-beta mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis. Dev Biol 341(1):186-95. [PubMed: 20193675] [MGI Ref ID J:160001]
Hosokawa R; Urata M; Han J; Zehnaly A; Bringas P Jr; Nonaka K; Chai Y. 2007. TGF-beta mediated Msx2 expression controls occipital somites-derived caudal region of skull development. Dev Biol 310(1):140-53. [PubMed: 17727833] [MGI Ref ID J:128003]
Hu JK; McGlinn E; Harfe BD; Kardon G; Tabin CJ. 2012. Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev 26(18):2088-102. [PubMed: 22987639] [MGI Ref ID J:187741]
Huh MS; Parker MH; Scime A; Parks R; Rudnicki MA. 2004. Rb is required for progression through myogenic differentiation but not maintenance of terminal differentiation. J Cell Biol 166(6):865-76. [PubMed: 15364961] [MGI Ref ID J:92502]
Huh MS; Price O'Dea T; Ouazia D; McKay BC; Parise G; Parks RJ; Rudnicki MA; Picketts DJ. 2012. Compromised genomic integrity impedes muscle growth after Atrx inactivation. J Clin Invest 122(12):4412-23. [PubMed: 23114596] [MGI Ref ID J:193998]
Klover P; Hennighausen L. 2007. Postnatal Body Growth Is Dependent on the Transcription Factors Signal Transducers and Activators of Transcription 5a/b in Muscle: A Role for Autocrine/Paracrine Insulin-Like Growth Factor I. Endocrinology 148(4):1489-97. [PubMed: 17158201] [MGI Ref ID J:119237]
Le Grand F; Grifone R; Mourikis P; Houbron C; Gigaud C; Pujol J; Maillet M; Pages G; Rudnicki M; Tajbakhsh S; Maire P. 2012. Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration. J Cell Biol 198(5):815-32. [PubMed: 22945933] [MGI Ref ID J:190460]
Le Grand F; Jones AE; Seale V; Scime A; Rudnicki MA. 2009. Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells. Cell Stem Cell 4(6):535-47. [PubMed: 19497282] [MGI Ref ID J:149821]
Li P; Pashmforoush M; Sucov HM. 2010. Retinoic acid regulates differentiation of the secondary heart field and TGFbeta-mediated outflow tract septation. Dev Cell 18(3):480-5. [PubMed: 20230754] [MGI Ref ID J:159110]
Martinez TL; Kong L; Wang X; Osborne MA; Crowder ME; Van Meerbeke JP; Xu X; Davis C; Wooley J; Goldhamer DJ; Lutz CM; Rich MM; Sumner CJ. 2012. Survival Motor Neuron Protein in Motor Neurons Determines Synaptic Integrity in Spinal Muscular Atrophy. J Neurosci 32(25):8703-8715. [PubMed: 22723710] [MGI Ref ID J:185657]
Nitzki F; Zibat A; Frommhold A; Schneider A; Schulz-Schaeffer W; Braun T; Hahn H. 2011. Uncommitted precursor cells might contribute to increased incidence of embryonal rhabdomyosarcoma in heterozygous Patched1-mutant mice. Oncogene 30(43):4428-36. [PubMed: 21602886] [MGI Ref ID J:178574]
Seale P; Bjork B; Yang W; Kajimura S; Chin S; Kuang S; Scime A; Devarakonda S; Conroe HM; Erdjument-Bromage H; Tempst P; Rudnicki MA; Beier DR; Spiegelman BM. 2008. PRDM16 controls a brown fat/skeletal muscle switch. Nature 454(7207):961-7. [PubMed: 18719582] [MGI Ref ID J:138823]
Shneider NA; Brown MN; Smith CA; Pickel J; Alvarez FJ. 2009. Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival. Neural Dev 4:42. [PubMed: 19954518] [MGI Ref ID J:160727]
Shneider NA; Mentis GZ; Schustak J; O'Donovan MJ. 2009. Functionally reduced sensorimotor connections form with normal specificity despite abnormal muscle spindle development: the role of spindle-derived neurotrophin 3. J Neurosci 29(15):4719-35. [PubMed: 19369542] [MGI Ref ID J:147957]
Tavares AL; Garcia EL; Kuhn K; Woods CM; Williams T; Clouthier DE. 2012. Ectodermal-derived Endothelin1 is required for patterning the distal and intermediate domains of the mouse mandibular arch. Dev Biol 371(1):47-56. [PubMed: 22902530] [MGI Ref ID J:190564]
Tchorz JS; Suply T; Ksiazek I; Giachino C; Cloetta D; Danzer CP; Doll T; Isken A; Lemaistre M; Taylor V; Bettler B; Kinzel B; Mueller M. 2012. A modified RMCE-compatible Rosa26 locus for the expression of transgenes from exogenous promoters. PLoS One 7(1):e30011. [PubMed: 22253858] [MGI Ref ID J:184312]
Tran TH; Shi X; Zaia J; Ai X. 2012. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem 287(39):32651-64. [PubMed: 22865881] [MGI Ref ID J:191590]
Vincent SD; Mayeuf A; Niro C; Saitou M; Buckingham M. 2012. Non Conservation of Function for the Evolutionarily Conserved Prdm1 Protein in the Control of the Slow Twitch Myogenic Program in the Mouse Embryo. Mol Biol Evol :. [PubMed: 22522309] [MGI Ref ID J:186268]
Waddell JN; Zhang P; Wen Y; Gupta SK; Yevtodiyenko A; Schmidt JV; Bidwell CA; Kumar A; Kuang S. 2010. Dlk1 is necessary for proper skeletal muscle development and regeneration. PLoS One 5(11):e15055. [PubMed: 21124733] [MGI Ref ID J:167124]
Watanabe S; Hirai H; Asakura Y; Tastad C; Verma M; Keller C; Dutton JR; Asakura A. 2011. MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells. Stem Cells 29(3):505-16. [PubMed: 21425413] [MGI Ref ID J:175957]
Watanabe S; Matsushita S; Hayasaka M; Hanaoka K. 2011. Generation of a conditional null allele of Lbx1. Genesis 49(10):803-10. [PubMed: 21956886] [MGI Ref ID J:178868]
Xiao H; Zhang X; Goodrich DW. 2010. Construction of a dual affinity tagged allele of the Rb1 tumor suppressor gene in the mouse. Genesis 48(2):121-6. [PubMed: 20027616] [MGI Ref ID J:158408]
Zhu H; Shyh-Chang N; Segre AV; Shinoda G; Shah SP; Einhorn WS; Takeuchi A; Engreitz JM; Hagan JP; Kharas MG; Urbach A; Thornton JE; Triboulet R; Gregory RI; Altshuler D; Daley GQ. 2011. The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell 147(1):81-94. [PubMed: 21962509] [MGI Ref ID J:177113]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred to wildtype siblings or to C57BL/6J. Homozygotes die at birth. Mating System +/+ sibling x Heterozygote (Female x Male) 31-DEC-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Myf5tm3(cre)Sor
Price per Pair (US dollars $) Pair Genotype $302.00 Heterozygous for Myf5tm3(cre)Sor x Wild-type for Myf5tm3(cre)Sor $302.00 Wild-type for Myf5tm3(cre)Sor x Heterozygous for Myf5tm3(cre)Sor Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Myf5tm3(cre)Sor
Price per Pair (US dollars $) Pair Genotype $392.60 Heterozygous for Myf5tm3(cre)Sor x Wild-type for Myf5tm3(cre)Sor $392.60 Wild-type for Myf5tm3(cre)Sor x Heterozygous for Myf5tm3(cre)Sor Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
|
|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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