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| Because the regulator of G-protein signaling 4 (Rgs4) gene is associated with neuropsychiatric disorders (including schizophrenia and Parkinson's), these RGS4 BAC transgenic (RGS4-GFP) mice may be useful for fluorescent reporting of RGS4 expression and over-expression of RGS4 in neurological studies investigating the role of RGS4 in the regulation of neuronal signaling. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation ?+N1F1 (20-OCT-08) Donating Investigator Pat Levitt, Vanderbilt University Description
Hemizygous RGS4 BAC transgenic mice are viable and fertile. As the RGS4 BAC transgene has an IRES2-eGFP construct inserted into the 3' UTR of the regulator of G-protein signaling 4 (Rgs4) locus, transgenic RGS4 transcripts and EGFP protein expression is observed in a pattern consistent with endogenous Rgs4. While the transgene is designed to co-express EGFP and RGS4, over-expression of RGS4 is not reported to result in unfaithful reporting of endogenous RGS4 expression. Under the control of the RGS4 promoter/enhancer elements, transgene expression reports dynamic developmental, regional, and cellular specific expression in developing and mature cerebral cortex neurons across all cortical domains, as well as developing and mature subcortical regions (telencephalon, diencephalon, and brainstem). While immunostaining against the transgenic product ("RGS4-GFP") allows detailed cellular resolution of neuronal cell bodies and processes, the subcellular localization of EGFP cannot be assumed to be identical to that of RGS4 (because the IRES2 region of the transgene directs individual RGS4 and EGFP expression (rather than a single fusion protein) and diffusion of EGFP throughout RGS4-expression cells results in a broader region of EGFP-immunolabeling than RGS4 transcript labeling). Because the regulator of G-protein signaling 4 (Rgs4) gene is associated with neuropsychiatric disorders (including schizophrenia and Parkinson's), these RGS4 BAC transgenic (RGS4-GFP) mice may be useful for fluorescent reporting of RGS4 expression and over-expression of RGS4 in neurological studies investigating the role of RGS4 in the regulation of neuronal signaling.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. The original publication(s) describe two founder lines (R4BAC3 and R4BAC4) on a mixed C57BL/6J;DBA genetic background with "virtually identical patterns of RGS4-GFP expression" in all lines, line R4BAC3 used to characterize the cerebral cortex phenotype, and both R4BAC3 and R4BAC4 lines used to characterize the subcortical brain areas. It should therefore be noted that the phenotype of these mice could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A C57BL/6J-derived bacterial artificial chromosome (BAC; RPCI-23-82i24) was modified by inserting an IRES2-eGFP construct (and an frt-flanked neomycin resistance cassette) into the 3' UTR of the Rgs4 locus. BAC DNA (approximately 225 kb) from correctly transformed and targeted bacteria (with the selection cassette excised and a single frt site remaining) was then injected into "C57BL/6J x DBA F1 embryos. Transgenic offspring (founder line 4 (R4BAC4)) were established on this B6;DBA mixed genetic background. These mice were subsequently backcrossed to C57BL/6J inbred mice for more than 12 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
005833 B6;129P2-Rgs4tm1Dgen/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Rgs4-EGFP)4Lvt/0
involves: C57BL/6 * DBA
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:128689)
- homozygotes are viable, fertile, and show no gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Parkinson's Disease (cell markers)
Parkinson's Disease
Receptor Defects (G protein-coupled receptor)
Research Tools
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Rgs4-EGFP)4Lvt | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Pat Levitt | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | R4BAC4; RGS4 BAC transgenic; RGS4 transgenic; RGS4-BAC; RGS4-BAC line 4; RGS4-GFP; | ||
| Mutation Made By | Philip Ebert, Vanderbilt University | ||
| Strain of Origin | (C57BL/6 x DBA)F1 | ||
| Site of Expression | EGFP expression shows dynamic developmental, regional, and cellular specific expression in developing and mature cerebral cortex neurons across all cortical domains, as well as developing and mature subcortical regions (telencephalon, diencephalon, and brainstem). | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Rgs4, regulator of G-protein signaling 4, mouse, laboratory | ||
| Molecular Note | A C57BL/6J-derived bacterial artificial chromosome (BAC; RPCI-23-82i24) was modified by inserting an IRES2-eGFP construct (and an frt-flanked neomycin resistance cassette) into the 3' UTR of the Rgs4 locus. The neomycin cassette was subsequently removed by FLP-mediated recombination. Lines 3 and 4 were developed, and line 4 was entered as the representative transgenic allele. Under the control of the RGS4 promoter/enhancer elements, transgene expression reports dynamic developmental, regional, and cellular specific expression in developing and mature cerebral cortex neurons across all cortical domains, as well as developing and mature subcortical regions (telencephalon, diencephalon, and brainstem). [MGI Ref ID J:128689] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Ebert PJ; Campbell DB; Levitt P. 2006. Bacterial artificial chromosome transgenic analysis of dynamic expression patterns of regulator of G-protein signaling 4 during development. I. Cerebral cortex. Neuroscience 142(4):1145-61. [PubMed: 16996696] [MGI Ref ID J:128689]
Ebert PJ; Campbell DB; Levitt P. 2006. Bacterial artificial chromosome transgenic analysis of dynamic expression patterns of regulator of G-protein signaling 4 during development. II. Subcortical regions. Neuroscience 142(4):1163-81. [PubMed: 16989953] [MGI Ref ID J:128690]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes are bred to wildtype siblings or C57BL/6J inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Rgs4-EGFP)4Lvt *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Rgs4-EGFP)4Lvt x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Rgs4-EGFP)4Lvt
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Rgs4-EGFP)4Lvt *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Rgs4-EGFP)4Lvt x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Rgs4-EGFP)4Lvt
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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