Strain Name: |
B6.Cg-Tg(Rgs4-EGFP)4Lvt/J |
|---|---|
Stock Number: |
007894 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | GFP; Rgs4; Tg(Rgs4-EGFP)4Lvt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Pat Levitt, Vanderbilt University Strain Description
Hemizygous RGS4 BAC transgenic mice are viable and fertile. As the RGS4 BAC transgene has an IRES2-eGFP construct inserted into the 3' UTR of the regulator of G-protein signaling 4 (Rgs4) locus, transgenic RGS4 transcripts and EGFP protein expression is observed in a pattern consistent with endogenous Rgs4. While the transgene is designed to co-express EGFP and RGS4, over-expression of RGS4 is not reported to result in unfaithful reporting of endogenous RGS4 expression. Under the control of the RGS4 promoter/enhancer elements, transgene expression reports dynamic developmental, regional, and cellular specific expression in developing and mature cerebral cortex neurons across all cortical domains, as well as developing and mature subcortical regions (telencephalon, diencephalon, and brainstem). While immunostaining against the transgenic product ("RGS4-GFP") allows detailed cellular resolution of neuronal cell bodies and processes, the subcellular localization of EGFP cannot be assumed to be identical to that of RGS4 (because the IRES2 region of the transgene directs individual RGS4 and EGFP expression (rather than a single fusion protein) and diffusion of EGFP throughout RGS4-expression cells results in a broader region of EGFP-immunolabeling than RGS4 transcript labeling). Because the regulator of G-protein signaling 4 (Rgs4) gene is associated with neuropsychiatric disorders (including schizophrenia and Parkinson's), these RGS4 BAC transgenic (RGS4-GFP) mice may be useful for fluorescent reporting of RGS4 expression and over-expression of RGS4 in neurological studies investigating the role of RGS4 in the regulation of neuronal signaling.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. The original publication(s) describe two founder lines (R4BAC3 and R4BAC4) on a mixed C57BL/6J;DBA genetic background with "virtually identical patterns of RGS4-GFP expression" in all lines, line R4BAC3 used to characterize the cerebral cortex phenotype, and both R4BAC3 and R4BAC4 lines used to characterize the subcortical brain areas. It should therefore be noted that the phenotype of these mice could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A C57BL/6J-derived bacterial artificial chromosome (BAC; RPCI-23-82i24) was modified by inserting an IRES2-eGFP construct (and an frt-flanked neomycin resistance cassette) into the 3' UTR of the Rgs4 locus. BAC DNA (approximately 225 kb) from correctly transformed and targeted bacteria (with the selection cassette excised and a single frt site remaining) was then injected into "C57BL/6J x DBA F1 embryos." Transgenic offspring (founder line 4 (R4BAC4)) were established on this B6;DBA mixed genetic background. These mice were subsequently backcrossed to C57BL/6J inbred mice for more than 12 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Rgs4-EGFP)4Lvt | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Pat Levitt | ||
| Common Name(s) | R4BAC4; RGS4 BAC transgenic; RGS4 transgenic; RGS4-BAC; RGS4-BAC line 4; RGS4-GFP; | ||
| Mutation Made By | Philip Ebert, Vanderbilt University | ||
| Strain of Origin | (C57BL/6 x DBA)F1 | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Rgs4, regulator of G-protein signaling 4, mouse, laboratory | ||
| Molecular Note | A C57BL/6J-derived bacterial artificial chromosome (BAC; RPCI-23-82i24) was modified by inserting an IRES2-eGFP construct (and an frt-flanked neomycin resistance cassette) into the 3' UTR of the Rgs4 locus. The neomycin cassette was subsequently removed by FLP-mediated recombination. Lines 3 and 4 were developed, and line 4 was entered as the representative transgenic allele. Under the control of the RGS4 promoter/enhancer elements, transgene expression reports dynamic developmental, regional, and cellular specific expression in developing and mature cerebral cortex neurons across all cortical domains, as well as developing and mature subcortical regions (telencephalon, diencephalon, and brainstem). [J:128689] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to wildtype siblings or C57BL/6J inbred mice. |
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Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Parkinson's Disease
Receptor Defects (G protein-coupled receptor)
Research Tools
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Ebert PJ; Campbell DB; Levitt P. 2006. Bacterial artificial chromosome transgenic analysis of dynamic expression patterns of regulator of G-protein signaling 4 during development. I. Cerebral cortex. Neuroscience 142(4):1145-61. [PubMed: 16996696] [J:128689]
Ebert PJ; Campbell DB; Levitt P. 2006. Bacterial artificial chromosome transgenic analysis of dynamic expression patterns of regulator of G-protein signaling 4 during development. II. Subcortical regions. Neuroscience 142(4):1163-81. [PubMed: 16989953] [J:128690]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Rgs4-EGFP)4Lvt/J |
| Stock Number: | 007894 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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