Strain Name: |
C57BL/6-Fastm1Cgn/J |
|---|---|
Stock Number: |
007895 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Fas; Fastm1Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Klaus Rajewsky, Immune Disease Institute (formerly CBRI) Generation F?+N1F1 (07-JUL-08) Strain Description
Mice homozygous for this "Fasfl" conditional allele are viable and fertile, with loxP sites flanking exon 9 of the targeted gene. When bred to mice that express Cre recombinase, exon 9 (which encodes the death domain) is deleted in the cre-expressing tissues in the resulting offspring.These Fasfl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, when Fasfl mice are crossed to a strain expressing Cre recombinase in B lineage cells (see Stock No. 004126 or 006785 ), this mutant mouse strain may be useful in studies of lymphoproliferative disorder. Similarly, when Fasfl mice are crossed to an interferon inducible strain with widespread Cre recombinase expression (see Stock No. 003556, 005673, or 002527), this mutant mouse strain may be useful in studies of lymphoproliferative disease. Further, deletion of Fas in T cells (by crossing Fasfl mice to a CD4-cre transgenic strain) produces mice that develop progressive lymphopenia and inflammatory pulmonary fibrosis which resembles idiopathic pulmonary fibrosis in humans.
Strain Development
A targeting vector was designed to place a loxP-flanked neo cassette downstream, and a third loxP site upstream of exon 9 of the targeted gene. The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing plasmid to remove the neo selection cassette. Chimeric mice were bred to C57BL/6 mice. The resulting "Fasfl" mice (with a single loxP site just upstream, and a single loxP site just downstream of exon 9) were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Fastm1Cgn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, University of Cologne | ||
| Common Name(s) | fasfl; | ||
| Mutation Made By | Klaus Rajewsky, Immune Disease Institute (formerly CBRI) | ||
| Strain of Origin | C57BL/6 | ||
| ES Cell Line Name | Bruce 4 | ||
| ES Cell Line Strain | C57BL/6 | ||
| Gene Symbol and Name | Fas, Fas (TNF receptor superfamily member 6) | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | AI196731; ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; Fas antigen; TNFR6; TNFRSF6; Tnfrsf6; expressed sequence AI196731; lpr; lymphoproliferation; tumor necrosis factor receptor superfamily, member 6; | ||
| Molecular Note | Exon 9, the death domain encoding exon, was flanked by loxP sites via homologous recombination. [MGI Ref ID J:114948] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fastm1Cgn
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Fas
003233 B6.129P2-Fastm1Osa/J 000482 B6.MRL-Faslpr/J 000480 C3.MRL-Faslpr/J 001876 CBA/KlJms-Faslpr-cg/J 002455 MRL-Faslpr.129P2(B6)-B2mtm1Unc 003234 MRL.129P2(B6)-Fastm1Osa/J 002983 MRL.CBAJms-Faslpr-cg/J 003896 MRL/MpJ Faslpr-Foxq1sa-J/J 006825 MRL/MpJ-Faslpr/2J 000485 MRL/MpJ-Faslpr/J 004519 NOD.MRL(C3)-Faslpr/DoiJ 004922 NOD.MRL-Faslpr/Dvs View Strains carrying other alleles of Fas (12 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Genes Regulating Growth and Proliferation
Research Tools
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Hematological Research
Immunology and Inflammation Research
References
Selected Reference(s)
Additional ReferencesHao Z; Hampel B; Yagita H; Rajewsky K. 2004. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis. J Exp Med 199(10):1355-65. [PubMed: 15148335] [MGI Ref ID J:114948]
Price and Supply Information
| Strain Name: | C57BL/6-Fastm1Cgn/J |
| Stock Number: | 007895 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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