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| These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients). | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Type Mutant Stock; Transgenic; Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation ?+F1 (12-MAY-08) Donating Investigator Andrew Leiter, Univ. of Massachusetts Medical School Description
Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).Development
The bacterial artificial chromosome (BAC) clone RP23-401D9 containing the mouse Gt(ROSA)26Sor gene (and 153 kb of upstream and 15 kb of downstream sequence) was modified by inserting a red-shifted variant Enhanced Green Fluorescent Protein (EGFP; Clontech No. 6029-1) cDNA and polyadenylation sequence at nucleotide 521 of the first exon of transcript 1. This ROSA26-EGFP BAC was microinjected into pronuclei of B6xB6D2F1 fertilized oocytes. Founders with a single copy of the transgene (founder line I1) were established and maintained by breeding with CD1 mice for at least 7 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (155 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
Tg(Gt(ROSA)26Sor-EGFP)I1Able/0
involves: C57BL/6 * CD-1 * DBA/2
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:126166)
- hemizygous mice are viable and fertile
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cancer Research (xenograft/transplant host)
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: cell marker for bone marrow transplantation)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Neurobiology Research (cell marker)
Reproductive Biology Research (transplantation marker for embryonic and adult tissue)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Gt(ROSA)26Sor-EGFP)I1Able | ||
|---|---|---|---|
| Allele Name | transgene insertion I1, Andrew B Leiter | ||
| Common Name(s) | ROSA26-EGFP; ROSA26-EGFP BAC; | ||
| Mutation Made By | Andrew Leiter, Univ. of Massachusetts Medical School | ||
| Strain of Origin | C57BL/6 x (C57BL/6 x DBA/2)F1 | ||
| Site of Expression | EGFP is expressed in all organs and tissues analyzed. Stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from these mice. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano, mouse, laboratory | ||
| Molecular Note | The bacterial artificial chromosome (BAC) clone RP23-401D9 containing the mouse Gt(ROSA)26Sor gene (and 153 kb of upstream and 15 kb of downstream sequence) was modified by inserting a red-shifted variant Enhanced Green Fluorescent Protein (EGFP; Clontech No. 6029-1) cDNA and polyadenylation sequence at nucleotide 521 of the first exon of transcript 1. Founders with a single copy of the transgene (founder line I1) were maintained on a CD-1 background. EGFP is ubiquitously and reproducibly expressed in all tissues and organs examined. Stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. [MGI Ref ID J:126166] | ||
| Gene Symbol and Name | Tg(Gt(ROSA)26Sor-EGFP)I1Able, transgene insertion I1, Andrew B Leiter | ||
| Chromosome | UN | ||
| Gene Common Name(s) | ROSA26-EGFP; ROSA26-EGFP BAC; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Gt(ROSA)26Sor-EGFP)I1Able, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Giel-Moloney M; Krause DS; Chen G; Van Etten RA; Leiter AB. 2007. Ubiquitous and uniform in vivo fluorescence in ROSA26-EGFP BAC transgenic mice. Genesis 45(2):83-9. [PubMed: 17269129] [MGI Ref ID J:126166]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to wildtype siblings. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Gt(ROSA)26Sor-EGFP)I1Able *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Gt(ROSA)26Sor-EGFP)I1Able x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Gt(ROSA)26Sor-EGFP)I1Able
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Gt(ROSA)26Sor-EGFP)I1Able *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Gt(ROSA)26Sor-EGFP)I1Able x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Gt(ROSA)26Sor-EGFP)I1Able
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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