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| The Cre-inducible expression of DTR in these iDTR mutant mice render cells susceptible to ablation following Diphtheria toxin administration. | ||||||||||||||||||||||
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Description
Strain Information
Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 15-OCT-08 Species laboratory mouse Generation F?+F11 (27-NOV-11)
Generation DefinitionsDonating Investigator Ari Waisman, Johannes Gutenberg University of Mainz Description
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.
When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 011062), this mutant mouse strain may be useful in studies of ovarian development.
When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. 011069), this mutant mouse strain may be useful in studies of metabolic dysfunction.
Of note, iDTR mice are also available on a BALB/cBy congenic background (as Stock No. 008040) and a NOD congenic background (as Stock No. 016603).
Development
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. The donating investigator reported that these iDTR mice were maintained as homozygotes on the C57BL/6 genetic background (see SNP note below) prior to arrival at The Jackson Laboratory.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(HBEGF)Awai allele
008040 CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J 016603 NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ View Strains carrying Gt(ROSA)26Sortm1(HBEGF)Awai (2 strains)
014176 C57BL/6-Tg(CLEC4C-HBEGF)956Cln/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Cd19tm1(cre)Cgn/? Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
involves: 129P2/OlaHsd * C57BL/6 (conditional)
- immune system phenotype
- abnormal lymph node cell ratio
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the lymph nodes (MGI Ref ID J:131076)
- abnormal lymphocyte morphology
- after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice (MGI Ref ID J:131076)
- abnormal splenic cell ratio
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen (MGI Ref ID J:131076)
- hematopoietic system phenotype
- abnormal bone marrow cell number
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed (MGI Ref ID J:131076)
- abnormal lymphocyte morphology
- after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice (MGI Ref ID J:131076)
- abnormal splenic cell ratio
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen (MGI Ref ID J:131076)
- cellular phenotype
- absent immature B cells
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed (MGI Ref ID J:131076)
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+ Tg(Gh1-cre)bKnmn/0
involves: C57BL/6 * FVB/N (conditional)
- homeostasis/metabolism phenotype
- abnormal circulating hormone level (MGI Ref ID J:169459)
- decreased circulating growth hormone level
- in diphtheria toxin-treated mice fed a high-fat or low-fat diet (MGI Ref ID J:169459)
- decreased circulating insulin level
- in diphtheria toxin-treated mice when a high-fat diet or a low-fat diet (MGI Ref ID J:169459)
- decreased circulating insulin-like growth factor I level
- in diphtheria toxin-treated mice fed a high-fat or low-fat diet (MGI Ref ID J:169459)
- increased circulating leptin level
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- abnormal energy expenditure (MGI Ref ID J:169459)
- decreased energy expenditure
- in diphtheria toxin-treated mice fed a low-fat during the nocturnal phase or when mice are fed a high-fat diet (MGI Ref ID J:169459)
- abnormal gas homeostasis (MGI Ref ID J:169459)
- abnormal glucose homeostasis (MGI Ref ID J:169459)
- decreased circulating glucose level
- decreased circulating insulin level
- in diphtheria toxin-treated mice when a high-fat diet or a low-fat diet (MGI Ref ID J:169459)
- impaired glucose tolerance
- in diphtheria toxin-treated mice fed a high-fat (MGI Ref ID J:169459)
- increased insulin sensitivity
- in diphtheria toxin-treated mice when fed standard chow, a high-fat diet, or a low-fat diet (MGI Ref ID J:169459)
- decreased liver triglyceride level
- in diphtheria toxin-treated mice fed a high-fat (MGI Ref ID J:169459)
- endocrine/exocrine gland phenotype
- decreased somatotroph cell number
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- small adenohypophysis
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- adipose tissue phenotype
- increased retroperitoneal fat pad weight
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- increased subcutaneous adipose tissue amount
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- increased total body fat amount
- in diphtheria toxin-treated mice fed standard chow or a high fat diet (MGI Ref ID J:169459)
- growth/size phenotype
- decreased body weight
- in diphtheria toxin-treated mice fed a high fat diet (MGI Ref ID J:169459)
- decreased lean body mass
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- liver/biliary system phenotype
- decreased liver triglyceride level
- in diphtheria toxin-treated mice fed a high-fat (MGI Ref ID J:169459)
- decreased liver weight
- in diphtheria toxin-treated mice fed standard chow or a high fat diet (MGI Ref ID J:169459)
- nervous system phenotype
- decreased somatotroph cell number
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- small adenohypophysis
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- behavior/neurological phenotype
- decreased fluid intake
- in diphtheria toxin-treated mice fed a low-fat (MGI Ref ID J:169459)
- integument phenotype
- increased subcutaneous adipose tissue amount
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai Tg(Gdf9-cre)5092Coo/0
involves: C57BL/6 (conditional)
- reproductive system phenotype
- abnormal ovary morphology (MGI Ref ID J:157008)
- abnormal ovarian follicle morphology
- with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis (MGI Ref ID J:157008)
- abnormal granulosa cell morphology
- cells have undergone apoptosis in preantral follicles (MGI Ref ID J:157008)
- oocyte degeneration
- oocytes have undergone apoptosis in preantral follicles (MGI Ref ID J:157008)
- endocrine/exocrine gland phenotype
- abnormal ovary morphology (MGI Ref ID J:157008)
- abnormal ovarian follicle morphology
- with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis (MGI Ref ID J:157008)
- abnormal granulosa cell morphology
- cells have undergone apoptosis in preantral follicles (MGI Ref ID J:157008)
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Research Tools
Cardiovascular Research
Cre-lox System
Cell Biology Research
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Diabetes and Obesity Research
loxP
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(HBEGF)Awai | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ari Waisman | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26iDTR; ROSA26-DTR; Rosa26iDTR; RosaiDTR; iDTR; | ||
| Mutation Made By | Ari Waisman, Johannes Gutenberg University of Mainz | ||
| Strain of Origin | B6.Cg-Thy1 | ||
| ES Cell Line Name | Bruce 4 | ||
| ES Cell Line Strain | B6.Cg-Thy1 | ||
| Expressed Gene | HBEGF, heparin-binding EGF-like growth factor, chimpanzee | ||
| General Note | Phenotypic Similarity to Human Syndrome: Isolated Growth Hormone Deficiency, Adult Onset (J:169459) | ||
| Molecular Note | A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. [MGI Ref ID J:131076] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(HBEGF)Awai, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920] [MGI Ref ID J:131076]
Additional References
Gt(ROSA)26Sortm1(HBEGF)Awai relatedArruda-Carvalho M; Sakaguchi M; Akers KG; Josselyn SA; Frankland PW. 2011. Posttraining ablation of adult-generated neurons degrades previously acquired memories. J Neurosci 31(42):15113-27. [PubMed: 22016545] [MGI Ref ID J:177636]
Chow A; Lucas D; Hidalgo A; Mendez-Ferrer S; Hashimoto D; Scheiermann C; Battista M; Leboeuf M; Prophete C; van Rooijen N; Tanaka M; Merad M; Frenette PS. 2011. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 208(2):261-71. [PubMed: 21282381] [MGI Ref ID J:176846]
Demehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676] [MGI Ref ID J:146637]
Dudeck A; Dudeck J; Scholten J; Petzold A; Surianarayanan S; Kohler A; Peschke K; Vohringer D; Waskow C; Krieg T; Muller W; Waisman A; Hartmann K; Gunzer M; Roers A. 2011. Mast Cells Are Key Promoters of Contact Allergy that Mediate the Adjuvant Effects of Haptens. Immunity 34(6):973-84. [PubMed: 21703544] [MGI Ref ID J:173991]
Durieux PF; Bearzatto B; Guiducci S; Buch T; Waisman A; Zoli M; Schiffmann SN; de Kerchove d'Exaerde A. 2009. D2R striatopallidal neurons inhibit both locomotor and drug reward processes. Nat Neurosci 12(4):393-5. [PubMed: 19270687] [MGI Ref ID J:150475]
Durieux PF; Schiffmann SN; de Kerchove d'Exaerde A. 2012. Differential regulation of motor control and response to dopaminergic drugs by D1R and D2R neurons in distinct dorsal striatum subregions. EMBO J 31(3):640-53. [PubMed: 22068054] [MGI Ref ID J:181813]
Fu Q; Gremeaux L; Luque RM; Liekens D; Chen J; Buch T; Waisman A; Kineman R; Vankelecom H. 2012. The adult pituitary shows stem/progenitor cell activation in response to injury and is capable of regeneration. Endocrinology 153(7):3224-35. [PubMed: 22518061] [MGI Ref ID J:188660]
Gahete MD; Cordoba-Chacon J; Luque RM; Kineman RD. 2013. The rise in growth hormone during starvation does not serve to maintain glucose levels or lean mass but is required for appropriate adipose tissue response in female mice. Endocrinology 154(1):263-9. [PubMed: 23150490] [MGI Ref ID J:193144]
Goren I; Allmann N; Yogev N; Schurmann C; Linke A; Holdener M; Waisman A; Pfeilschifter J; Frank S. 2009. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol 175(1):132-47. [PubMed: 19528348] [MGI Ref ID J:150035]
Han JH; Kushner SA; Yiu AP; Hsiang HL; Buch T; Waisman A; Bontempi B; Neve RL; Frankland PW; Josselyn SA. 2009. Selective erasure of a fear memory. Science 323(5920):1492-6. [PubMed: 19286560] [MGI Ref ID J:145905]
Hatori M; Le H; Vollmers C; Keding SR; Tanaka N; Schmedt C; Jegla T; Panda S. 2008. Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3(6):e2451. [PubMed: 18545654] [MGI Ref ID J:137151]
Landsman L; Nijagal A; Whitchurch TJ; Vanderlaan RL; Zimmer WE; Mackenzie TC; Hebrok M. 2011. Pancreatic mesenchyme regulates epithelial organogenesis throughout development. PLoS Biol 9(9):e1001143. [PubMed: 21909240] [MGI Ref ID J:182781]
Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217] [MGI Ref ID J:186240]
Lucas T; Waisman A; Ranjan R; Roes J; Krieg T; Muller W; Roers A; Eming SA. 2010. Differential roles of macrophages in diverse phases of skin repair. J Immunol 184(7):3964-77. [PubMed: 20176743] [MGI Ref ID J:160100]
Luque RM; Lin Q; Cordoba-Chacon J; Subbaiah PV; Buch T; Waisman A; Vankelecom H; Kineman RD. 2011. Metabolic impact of adult-onset, isolated, growth hormone deficiency (AOiGHD) due to destruction of pituitary somatotropes. PLoS One 6(1):e15767. [PubMed: 21283519] [MGI Ref ID J:169459]
Mayer C; Boehm U. 2011. Female reproductive maturation in the absence of kisspeptin/GPR54 signaling. Nat Neurosci 14(6):704-10. [PubMed: 21516099] [MGI Ref ID J:173936]
Mendez-Ferrer S; Michurina TV; Ferraro F; Mazloom AR; Macarthur BD; Lira SA; Scadden DT; Ma'ayan A; Enikolopov GN; Frenette PS. 2010. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 466(7308):829-34. [PubMed: 20703299] [MGI Ref ID J:163310]
Nakanishi Y; Seno H; Fukuoka A; Ueo T; Yamaga Y; Maruno T; Nakanishi N; Kanda K; Komekado H; Kawada M; Isomura A; Kawada K; Sakai Y; Yanagita M; Kageyama R; Kawaguchi Y; Taketo MM; Yonehara S; Chiba T. 2013. Dclk1 distinguishes between tumor and normal stem cells in the intestine. Nat Genet 45(1):98-103. [PubMed: 23202126] [MGI Ref ID J:193873]
Narni-Mancinelli E; Chaix J; Fenis A; Kerdiles YM; Yessaad N; Reynders A; Gregoire C; Luche H; Ugolini S; Tomasello E; Walzer T; Vivier E. 2011. Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor. Proc Natl Acad Sci U S A 108(45):18324-9. [PubMed: 22021440] [MGI Ref ID J:180265]
Onder L; Narang P; Scandella E; Chai Q; Iolyeva M; Hoorweg K; Halin C; Richie E; Kaye P; Westermann J; Cupedo T; Coles M; Ludewig B. 2012. IL-7-producing stromal cells are critical for lymph node remodeling. Blood 120(24):4675-83. [PubMed: 22955921] [MGI Ref ID J:192130]
Owens BM; Beattie L; Moore JW; Brown N; Mann JL; Dalton JE; Maroof A; Kaye PM. 2012. IL-10-producing Th1 cells and disease progression are regulated by distinct CD11c(+) cell populations during visceral leishmaniasis. PLoS Pathog 8(7):e1002827. [PubMed: 22911108] [MGI Ref ID J:195372]
Park D; Spencer JA; Koh BI; Kobayashi T; Fujisaki J; Clemens TL; Lin CP; Kronenberg HM; Scadden DT. 2012. Endogenous bone marrow MSCs are dynamic, fate-restricted participants in bone maintenance and regeneration. Cell Stem Cell 10(3):259-72. [PubMed: 22385654] [MGI Ref ID J:185671]
Sharma MD; Hou DY; Baban B; Koni PA; He Y; Chandler PR; Blazar BR; Mellor AL; Munn DH. 2010. Reprogrammed foxp3(+) regulatory T cells provide essential help to support cross-presentation and CD8(+) T cell priming in naive mice. Immunity 33(6):942-54. [PubMed: 21145762] [MGI Ref ID J:167291]
Thirumangalathu S; Harlow DE; Driskell AL; Krimm RF; Barlow LA. 2009. Fate mapping of mammalian embryonic taste bud progenitors. Development 136(9):1519-28. [PubMed: 19363153] [MGI Ref ID J:147959]
Uhlenhaut NH; Jakob S; Anlag K; Eisenberger T; Sekido R; Kress J; Treier AC; Klugmann C; Klasen C; Holter NI; Riethmacher D; Schutz G; Cooney AJ; Lovell-Badge R; Treier M. 2009. Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. Cell 139(6):1130-42. [PubMed: 20005806] [MGI Ref ID J:157008]
Wang S; Ware SM. 2009. Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression. Genesis 47(2):132-6. [PubMed: 19165828] [MGI Ref ID J:147303]
Wang X; Cho B; Suzuki K; Xu Y; Green JA; An J; Cyster JG. 2011. Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers. J Exp Med 208(12):2497-510. [PubMed: 22042977] [MGI Ref ID J:178640]
Wenzel P; Knorr M; Kossmann S; Stratmann J; Hausding M; Schuhmacher S; Karbach SH; Schwenk M; Yogev N; Schulz E; Oelze M; Grabbe S; Jonuleit H; Becker C; Daiber A; Waisman A; Munzel T. 2011. Lysozyme M-positive monocytes mediate angiotensin II-induced arterial hypertension and vascular dysfunction. Circulation 124(12):1370-81. [PubMed: 21875910] [MGI Ref ID J:189377]
Yi T; Wang X; Kelly LM; An J; Xu Y; Sailer AW; Gustafsson JA; Russell DW; Cyster JG. 2012. Oxysterol Gradient Generation by Lymphoid Stromal Cells Guides Activated B Cell Movement during Humoral Responses. Immunity 37(3):535-48. [PubMed: 22999953] [MGI Ref ID J:188276]
Yogev N; Frommer F; Lukas D; Kautz-Neu K; Karram K; Ielo D; von Stebut E; Probst HC; van den Broek M; Riethmacher D; Birnberg T; Blank T; Reizis B; Korn T; Wiendl H; Jung S; Prinz M; Kurschus FC; Waisman A. 2012. Dendritic Cells Ameliorate Autoimmunity in the CNS by Controlling the Homeostasis of PD-1 Receptor(+) Regulatory T Cells. Immunity 37(2):264-75. [PubMed: 22902234] [MGI Ref ID J:187367]
van Montfoort N; Mangsbo SM; Camps MG; van Maren WW; Verhaart IE; Waisman A; Drijfhout JW; Melief CJ; Verbeek JS; Ossendorp F. 2012. Circulating specific antibodies enhance systemic cross-priming by delivery of complexed antigen to dendritic cells in vivo. Eur J Immunol 42(3):598-606. [PubMed: 22488363] [MGI Ref ID J:187782]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 15-OCT-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $195.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Price per Pair (US dollars $) Pair Genotype $390.00 Homozygous for Gt(ROSA)26Sortm1(HBEGF)Awai x Homozygous for Gt(ROSA)26Sortm1(HBEGF)Awai Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $253.50 Female or Male Homozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Price per Pair (US dollars $) Pair Genotype $507.00 Homozygous for Gt(ROSA)26Sortm1(HBEGF)Awai x Homozygous for Gt(ROSA)26Sortm1(HBEGF)Awai Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
|
|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.