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Strain Name:

C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J

Stock Number:

007900

Availability:

Under Development for Distribution Colony

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General Terms and Conditions
Additional Licensing and Use Restrictions

Strain Common Names      B6-iDTR;
Genes & Alleles   Gt(ROSA)26Sor;   Gt(ROSA)26Sortm1(HBEGF)Awai;

Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHeterozygote x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Ari Waisman,   Johannes Gutenberg University of Mainz
GenerationF?+F1 (25-JUN-08)

Strain Description
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.

Strain Development
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. These iDTR mice were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Cd19tm1(cre)Cgn/? Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai

        involves: 129P2/OlaHsd * C57BL/6   (conditional)
  • immune system phenotype
  • abnormal lymph node cellularity (MGI Ref ID J:131076)
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the lymph nodes
  • abnormal lymphocyte morphology (MGI Ref ID J:131076)
    • after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice
    • abnormal B cell morphology (MGI Ref ID J:131076)
      • CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
    • abnormal lymphocyte cell number (MGI Ref ID J:131076)
      • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
  • abnormal spleen cellularity (MGI Ref ID J:131076)
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen
  • hematopoietic system phenotype
  • abnormal bone marrow cell number (MGI Ref ID J:131076)
    • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
  • abnormal lymphocyte morphology (MGI Ref ID J:131076)
    • after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice
    • abnormal B cell morphology (MGI Ref ID J:131076)
      • CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
    • abnormal lymphocyte cell number (MGI Ref ID J:131076)
      • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
  • abnormal spleen cellularity (MGI Ref ID J:131076)
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen

Gene & Allele Details

Allele Symbol Gt(ROSA)26Sortm1(HBEGF)Awai
Allele Name targeted mutation 1, Ari Waisman
Common Name(s) R26iDTR; ROSA26-DTR; iDTR;
Mutation Made By Ari Waisman,   Johannes Gutenberg University of Mainz
Strain of OriginC57BL/6
ES Cell Line NameBruce 4
ES Cell Line StrainC57BL/6
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;
Molecular Note A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. [MGI Ref ID J:131076] [MGI Ref ID J:64292]

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Gt(ROSA)26Sortm1(HBEGF)Awai

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003310   129S-Gt(ROSA)26Sortm1Sor/J
003946   129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
002073   B6;129S-Gt(ROSA)26Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
002955   C.129S7-Gt(ROSA)26Sor/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm1(HIF1A/luc)Kael/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
008159   STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (36 strains)

Additional Web Information

Cre-lox or FLP-FRT Systems

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (loxP-flanked Sequences)

Research Tools
Cardiovascular Research (Cre-lox System)
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research (loxP)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Reproductive Biology Research (Cre-lox System)

References

Selected Reference(s)

Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920]  [MGI Ref ID J:131076]

Additional References

Price and Supply Information

Strain Name: C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
Stock Number: 007900
 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 08-SEP-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard SupplyUnder Development for Distribution Colony
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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