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| These Brainbow 1.0 (founder line H) mice allow labeling of individual neuronal types (including motor neurons, dentate gyrus granule cells, pyramidal neurons of the cortex and CA1 area) with approximately 90 distinguishable color variations in cre recombined cells, and may also be useful in conjunction with other Brainbow strains (Stock No. 007910, Stock No. 007911, Stock No. 007921) for neurobiological studies. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6.Cg-Tg(Thy1-tdimer2(12),-mYFP,-mCerulean)H10Lich/J (Changed: 15-NOV-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Hemizygote (Female x Male) 23-OCT-08 Species laboratory mouse Generation N5?N1F14 (06-MAR-13)
Generation DefinitionsDonating Investigator Joshua R Sanes, Harvard University Donating Investigator Jeff Lichtman, Harvard University Description
View Brainbow images for Stock No's 007901, 007910, 007911, and 007921.
These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the single FRT site inserted in the transgene allows tandem transgene copy number reduction through Flp-mediated recombination if desired. These Brainbow 1.0 (founder line H) mice were found to have 8 transgene copies that allow labeling of individual neuronal types (including motor neurons, dentate gyrus granule cells, pyramidal neurons of the cortex and CA1 area) with approximately 90 distinguishable color variations in cre recombined cells, and may also be useful in conjunction with other Brainbow strains (Stock No. 007910, Stock No. 007911, Stock No. 007921) for neurobiological studies.Development
The Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), enhanced yellow (EYFP), or cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5' to 3') a Lox2272 site, LoxP site, tdimer2(12) RFP (with polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5' FRT site and polyA sequence). This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. Founder mice (line H) were bred with C57BL/6. The resulting mutant mice were backcrossed to C57BL/6 for at least 5 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. After many generations of breeding hemizygous mice together at The Jackson Laboratory, only females have shown to be homozygous for the transgene; this suggests the transgene is X chromosome-linked.The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer). The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 014131 B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 013161 B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 018671 D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ 018067 FVB-Tg(Prism)1849Htz/J 018068 FVB-Tg(Prism)1989Htz/J 007879 STOCK Stx1atm2Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-Cerulean)24Hara/J 016922 STOCK Tg(Myh7-CFP)1Jrs/J 013162 STOCK Tg(Thy1-Clomeleon)12Gjau/J 013163 STOCK Tg(Thy1-Clomeleon)13Gjau/J
Additional Web Information
FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
JAX® NOTES, Winter 2008; 508. Brainbow Mice Soon to be Available From The Jackson Laboratory.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
transplantation marker for embryonic and adult tissue
FLP-FRT System
FRT-flanked Sequences
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: astrocyte-specific marker
Tissue/Cell Markers: astrocytes
Tissue/Cell Markers: astrocytes, neurons
Tissue/Cell Markers: multiple
Tissue/Cell Markers: neurons
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
astrocyte-specific marker
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-Brainbow1.0)HLich | ||
|---|---|---|---|
| Allele Name | transgene insertion H, Jeff W Lichtman | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(Thy1-tdimer2(12),-mYFP,-mCerulean)H10Lich; Thy1-Brainbow-1.0 line H; | ||
| Mutation Made By | Jeff Lichtman and Joshua Sanes, Harvard University | ||
| Strain of Origin | C57BL/6 x CBA | ||
| Site of Expression | tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, there is one of three expression outcomes for each transgene copy in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). Integration of 8 tandem transgene copies allows for labeling of individual neurons and glia with approximately 90 distinguishable color variations in cre recombined cells. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), enhanced yellow (EYFP), or cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5'to 3') a Lox2272 site, LoxP site, tdimer2(12) RFP (with polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5'FRT site and polyA sequence). This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copiesof the protein (tandem dimer). The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. [MGI Ref ID J:125961] | ||
| Gene Symbol and Name | Tg(Thy1-Brainbow1.0)HLich, transgene insertion H, Jeff W Lichtman | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Thy1-tdimer2(12),-mYFP,-mCerulean)H10Lich; Thy1-Brainbow-1.0 line H; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins -- Generic GFP, QPCR
Tg(Thy1-Brainbow1.0)HLich, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. [PubMed: 17972876] [MGI Ref ID J:125961]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry After many generations of breeding hemizygous mice together at The Jackson Laboratory, only females have shown to be homozygous for the transgene; this suggests the transgene is X chromosome-linked. When maintaining a live colony, homozygous females may be bred to hemizygous males. Mating System Homozygote x Hemizygote (Female x Male) 23-OCT-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Male Hemizygous for Tg(Thy1-Brainbow1.0)HLich $232.00 Female Homozygous for Tg(Thy1-Brainbow1.0)HLich
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(Thy1-Brainbow1.0)HLich x Hemizygous for Tg(Thy1-Brainbow1.0)HLich Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Male Hemizygous for Tg(Thy1-Brainbow1.0)HLich $301.60 Female Homozygous for Tg(Thy1-Brainbow1.0)HLich
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(Thy1-Brainbow1.0)HLich x Hemizygous for Tg(Thy1-Brainbow1.0)HLich Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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