Strain Name: |
B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J |
|---|---|
Stock Number: |
007902 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Chat; GFP; Tg(RP23-268L19-EGFP)2Mik; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Michael Kotlikoff, Cornell University Generation N6+F0 (15-MAY-08) Strain Description
Mice hemizygous for the ChAT(BAC)-eGFP (ChATBAC-eGFP) transgene are viable and fertile, with the endogenous choline acetyltransferase (ChAT) transcriptional regulatory elements (cholinergic gene locus) directing enhanced green fluorescent protein (EGFP) protein expression during development as well as in the adult mouse. As such, EGFP is expressed in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems and neuromuscular junctions. These ChAT(BAC)-eGFP transgenic mice allow fluorescent visualization of cholinergic elements of the central and peripheral nervous system and may be useful for studying cholinergic neurotransmission and neuromuscular coupling.Strain Development
The bacterial artificial chromosome (BAC) clone RP23-268L19 (containing the mouse choline acetyltransferase (ChAT) locus and approximately 78 kb of 3’- and 36 kb of 5’-flanking DNA), was modified to insert an enhanced green fluorescent protein (EGFP) gene with polyA sequence at the initiation codon in ChAT exon 3. The resulting BAC (ChATBAC-eGFP; with a single FRT site downstream of EGFP-polyA) was microinjected into the pronucleus of B6D2F2 fertilized mouse eggs. Transgenic mice were bred to C57BL/6J, and the resulting transgenic offspring (ChAT founder line 2) were established. These ChAT(BAC)-eGFP mice were backcrossed to C57BL/6J mice for at least 4 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation.
Gene & Allele Details
| Allele Symbol | Tg(RP23-268L19-EGFP)2Mik | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Michael I Kotlikoff | ||
| Common Name(s) | ChAT line 2; ChAT(BAC)-eGFP; ChATBAC-eGFP; Tg(RP23-268L19-eGFP)Kot; | ||
| Mutation Made By | Michael Kotlikoff, Cornell University | ||
| Site of Expression | EGFP is expressed in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems and neuromuscular junctions. | ||
| Expressed Gene | Chat, choline acetyltransferase, mouse, laboratory | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Chat, choline acetyltransferase, mouse, laboratory | ||
| General Note | Transgenic lines were derived from two transgenic founder mice and designated line 2 and line 44. No apparent differences were observed in their systemic patterns of EGFP expression either by fluorescent imaging or by immunohistochemical analysis. | ||
| Molecular Note | The transgene consists of the bacterial artificial chromosome (BAC) clone 268L19 from the RP23 mouse genomic library, which contains the cholinesterase acetyltransferase (Chat) gene, in whose first intron resides the Sl18a3 (solute carrierfamily 18 (vesicular monoamine)) gene encoding the vesicular acetylcholine transporter (VAChT); ~78 kb of 5' and ~36 kb of 3' flanking DNA; and, inserted in place of the Chat translation initiation codon in exon 3, the enhanced green fluorescent protein (EGFP) gene followed by a polyadenylation signal. EGFP fluorescence is observed in cell bodies and processes of cholinergic neurons of both the central and peripheral nervous systems, including somatic motor and sensory neurons, parasympathetic neurons of various peripheral organ systems, and ganglionic neurons. Individual neuromuscular junctions of cardiac, skeletal and smooth muscle can be visualized. Individual gastrointestinal and respiratory mucosal epithelial cells and some associated lymphocytes also fluoresce. [MGI Ref ID J:114554] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(RP23-268L19-EGFP)2Mik
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous ChAT(BAC)-eGFP mice can be bred to wildtype siblings or C57BL/6J inbred mice. |
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| Diet Information | LabDiet® 5K52/5K67 |
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006410 B6;129S6-Chattm1(cre)Lowl/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: astrocytes)
Genetics Research (Tissue/Cell Markers: astrocytes, neurons)
Genetics Research (Tissue/Cell Markers: dorsal CNS marker)
Genetics Research (Tissue/Cell Markers: glial cells)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: neurons)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Tallini YN; Shui B; Greene KS; Deng KY; Doran R; Fisher PJ; Zipfel W; Kotlikoff MI. 2006. BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons. Physiol Genomics 27(3):391-7. [PubMed: 16940431] [MGI Ref ID J:114554]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J |
| Stock Number: | 007902 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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