| |||||||||||||||
| These ChAT(BAC)-eGFP transgenic mice allow fluorescent visualization of cholinergic elements of the central and peripheral nervous system and may be useful for studying cholinergic neurotransmission and neuromuscular coupling. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 17-JUL-09 Species laboratory mouse Generation N6+F2 (03-DEC-08) Donating Investigator Michael Kotlikoff, Cornell University Description
Mice hemizygous for the ChAT(BAC)-eGFP (ChATBAC-eGFP) transgene are viable and fertile, with the endogenous choline acetyltransferase (ChAT) transcriptional regulatory elements (cholinergic gene locus) directing enhanced green fluorescent protein (EGFP) protein expression during development as well as in the adult mouse. As such, EGFP is expressed in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems and neuromuscular junctions. These ChAT(BAC)-eGFP transgenic mice allow fluorescent visualization of cholinergic elements of the central and peripheral nervous system and may be useful for studying cholinergic neurotransmission and neuromuscular coupling.Development
The bacterial artificial chromosome (BAC) clone RP23-268L19 (containing the mouse choline acetyltransferase (ChAT) locus and approximately 78 kb of 3’- and 36 kb of 5’-flanking DNA), was modified to insert an enhanced green fluorescent protein (EGFP) gene with polyA sequence at the initiation codon in ChAT exon 3. The resulting BAC (ChATBAC-eGFP; with a single FRT site downstream of EGFP-polyA) was microinjected into the pronucleus of B6D2F2 fertilized mouse eggs. Transgenic mice were bred to C57BL/6J, and the resulting transgenic offspring (ChAT founder line 2) were established. These ChAT(BAC)-eGFP mice were backcrossed to C57BL/6J mice for at least 4 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
008364 B6;129-Chattm1(cre/Esr1)Nat/J 006410 B6;129S6-Chattm1(cre)Lowl/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: astrocytes
Tissue/Cell Markers: astrocytes, neurons
Tissue/Cell Markers: dorsal CNS marker
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: multiple
Tissue/Cell Markers: neurons
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(RP23-268L19-EGFP)2Mik | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Michael I Kotlikoff | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | ChAT line 2; ChAT(BAC)-eGFP; ChATBAC-eGFP; Tg(RP23-268L19-eGFP)Kot; | ||
| Mutation Made By | Michael Kotlikoff, Cornell University | ||
| Site of Expression | EGFP is expressed in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems and neuromuscular junctions. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Chat, choline acetyltransferase, mouse, laboratory | ||
| Promoter | Chat, choline acetyltransferase, mouse, laboratory | ||
| General Note | Transgenic lines were derived from two transgenic founder mice and designated line 2 and line 44. No apparent differences were observed in their systemic patterns of EGFP expression either by fluorescent imaging or by immunohistochemical analysis. | ||
| Molecular Note | The transgene consists of the bacterial artificial chromosome (BAC) clone 268L19 from the RP23 mouse genomic library, which contains the cholinesterase acetyltransferase (Chat) gene, in whose first intron resides the Sl18a3 (solute carrierfamily 18 (vesicular monoamine)) gene encoding the vesicular acetylcholine transporter (VAChT); ~78 kb of 5' and ~36 kb of 3' flanking DNA; and, inserted in place of the Chat translation initiation codon in exon 3, the enhanced green fluorescent protein (EGFP) gene followed by a polyadenylation signal. EGFP fluorescence is observed in cell bodies and processes of cholinergic neurons of both the central and peripheral nervous systems, including somatic motor and sensory neurons, parasympathetic neurons of various peripheral organ systems, and ganglionic neurons. Individual neuromuscular junctions of cardiac, skeletal and smooth muscle can be visualized. Individual gastrointestinal and respiratory mucosal epithelial cells and some associated lymphocytes also fluoresce. [MGI Ref ID J:114554] | ||
| Gene Symbol and Name | Tg(RP23-268L19-EGFP)2Mik, transgene insertion 2, Michael I Kotlikoff | ||
| Chromosome | UN | ||
| Gene Common Name(s) | ChAT line 2; ChAT(BAC)-eGFP; ChATBAC-eGFP; Tg(RP23-268L19-eGFP)Kot; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(RP23-268L19-EGFP)2Mik, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Tallini YN; Shui B; Greene KS; Deng KY; Doran R; Fisher PJ; Zipfel W; Kotlikoff MI. 2006. BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons. Physiol Genomics 27(3):391-7. [PubMed: 16940431] [MGI Ref ID J:114554]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous ChAT(BAC)-eGFP mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 17-JUL-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(RP23-268L19-EGFP)2Mik
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(RP23-268L19-EGFP)2Mik x Homozygous for Tg(RP23-268L19-EGFP)2Mik
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(RP23-268L19-EGFP)2Mik
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(RP23-268L19-EGFP)2Mik x Homozygous for Tg(RP23-268L19-EGFP)2Mik
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.