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| These Ai9 mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato), and may be useful as a Cre reporter strain. TdTomato is expressed following Cre-mediated recombination. These mice are available on a B6;129S6 mixed genetic background (Stock No. 007905) and a C57BL/6J congenic background (Stock No. 007909). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Gene Trap; Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 10-SEP-09 Species laboratory mouse Generation N1+N1F6 (11-MAY-11)
Generation DefinitionsDonating Investigator Hongkui Zeng, Allen Institute for Brain Science (AIBS) Description
Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if so desired. Similarly, the attB/attP-flanked selection cassette may be removed by introduction of the site-specific bacteriophage PhiC31 integrase if so desired. These Ai9 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.The Allen Institute for Brain Science (AIBS) website has specific characterization information for several Cre Driver and Cre Reporter lines. Please see their website for images of AIBS experiments performed with all lines.
Of note, Ai9 mice may also be available on a C57BL/6J congenic background (see Stock No. 007909).
Development
The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation of (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells (clone Ai9) were selected and chimeric males were bred to C57BL/6J females. The resulting Ai9 mice were then backcrossed to C57BL/6J for at least 1 generation prior to arrival at The Jackson Laboratory. These Ai9 mice on a B6;129S6 mixed genetic background are designated Stock No. 007905.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Allen Institute for Brain Science cre reporter strains
View Allen Institute for Brain Science cre reporter strains (15 strains)
007909 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
Additional Web Information
FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+
involves: 129S6/SvEvTac * C57BL/6NCr
- normal phenotype
- no abnormal phenotype detected
- mice are viable and fertile; used for cre reporter expression analysis (MGI Ref ID J:155793)
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
transplantation marker for embryonic and adult tissue
Diabetes and Obesity Research
loxP
FLP-FRT System
FRT-flanked Sequences
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: cell marker for bone marrow transplantation
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Reproductive Biology Research
Cre-lox System
transplantation marker for embryonic and adult tissue
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm9(CAG-tdTomato)Hze | ||
|---|---|---|---|
| Allele Name | targeted mutation 9, Hongkui Zeng | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Ai9; Rosa-CAG-LSL-tdTomato-WPRE; Rosa-LSL-TdTomato; | ||
| Mutation Made By | Hongkui Zeng, Allen Institute for Brain Science (AIBS) | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| ES Cell Line Name | G4 | ||
| ES Cell Line Strain | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| Site of Expression | A floxed STOP prevents transcription of red fluorescent protein variant (tdTomato) until mice are bred to a Cre strain. Resulting offspring express tdTomato in a pattern dictated by the Cre promoter. | ||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Molecular Note | The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. [MGI Ref ID J:155793] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor(CAG-tdTomato), Melt Curve Analysis
Gt(ROSA)26Sortm9(CAG-tdTomato)Hze, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40. [PubMed: 20023653] [MGI Ref ID J:155793]
Additional References
Gt(ROSA)26Sortm9(CAG-tdTomato)Hze relatedChee MJ; Pissios P; Maratos-Flier E. 2013. Neurochemical charaterization of neurons expressing melanin-concentrating hormone receptor 1 in the mouse hypothalamus J Comp Neurol :. [MGI Ref ID J:194588]
Clement JP; Aceti M; Creson TK; Ozkan ED; Shi Y; Reish NJ; Almonte AG; Miller BH; Wiltgen BJ; Miller CA; Xu X; Rumbaugh G. 2012. Pathogenic SYNGAP1 mutations impair cognitive development by disrupting maturation of dendritic spine synapses. Cell 151(4):709-23. [PubMed: 23141534] [MGI Ref ID J:193208]
El Agha E; Al Alam D; Carraro G; MacKenzie B; Goth K; De Langhe SP; Voswinckel R; Hajihosseini MK; Rehan VK; Bellusci S. 2012. Characterization of a novel fibroblast growth factor 10 (Fgf10) knock-in mouse line to target mesenchymal progenitors during embryonic development. PLoS One 7(6):e38452. [PubMed: 22719891] [MGI Ref ID J:187831]
Flesken-Nikitin A; Hwang CI; Cheng CY; Michurina TV; Enikolopov G; Nikitin AY. 2013. Ovarian surface epithelium at the junction area contains a cancer-prone stem cell niche. Nature 495(7440):241-5. [PubMed: 23467088] [MGI Ref ID J:194916]
Garfield AS; Patterson C; Skora S; Gribble FM; Reimann F; Evans ML; Myers MG Jr; Heisler LK. 2012. Neurochemical characterization of body weight-regulating leptin receptor neurons in the nucleus of the solitary tract. Endocrinology 153(10):4600-7. [PubMed: 22869346] [MGI Ref ID J:191291]
Grcevic D; Pejda S; Matthews BG; Repic D; Wang L; Li H; Kronenberg MS; Jiang X; Maye P; Adams DJ; Rowe DW; Aguila HL; Kalajzic I. 2012. In vivo fate mapping identifies mesenchymal progenitor cells. Stem Cells 30(2):187-96. [PubMed: 22083974] [MGI Ref ID J:190191]
Greenbaum A; Hsu YM; Day RB; Schuettpelz LG; Christopher MJ; Borgerding JN; Nagasawa T; Link DC. 2013. CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance. Nature 495(7440):227-30. [PubMed: 23434756] [MGI Ref ID J:193594]
Hafler BP; Surzenko N; Beier KT; Punzo C; Trimarchi JM; Kong JH; Cepko CL. 2012. Transcription factor Olig2 defines subpopulations of retinal progenitor cells biased toward specific cell fates. Proc Natl Acad Sci U S A 109(20):7882-7. [PubMed: 22543161] [MGI Ref ID J:184797]
Lee YH; Petkova AP; Mottillo EP; Granneman JG. 2012. In vivo identification of bipotential adipocyte progenitors recruited by beta3-adrenoceptor activation and high-fat feeding. Cell Metab 15(4):480-91. [PubMed: 22482730] [MGI Ref ID J:184459]
Lehoczky JA; Robert B; Tabin CJ. 2011. Mouse digit tip regeneration is mediated by fate-restricted progenitor cells. Proc Natl Acad Sci U S A 108(51):20609-14. [PubMed: 22143790] [MGI Ref ID J:180520]
Macfarlan TS; Gifford WD; Driscoll S; Lettieri K; Rowe HM; Bonanomi D; Firth A; Singer O; Trono D; Pfaff SL. 2012. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature 487(7405):57-63. [PubMed: 22722858] [MGI Ref ID J:186302]
Mishra SK; Tisel SM; Orestes P; Bhangoo SK; Hoon MA. 2011. TRPV1-lineage neurons are required for thermal sensation. EMBO J 30(3):582-93. [PubMed: 21139565] [MGI Ref ID J:169256]
Nickerson PE; Ronellenfitch K; McEwan J; Kim H; McInnes RR; Chow RL. 2011. A transgenic mouse line expressing cre recombinase in undifferentiated postmitotic mouse retinal bipolar cell precursors. PLoS One 6(10):e27145. [PubMed: 22073130] [MGI Ref ID J:178063]
Ohtsuki G; Nishiyama M; Yoshida T; Murakami T; Histed M; Lois C; Ohki K. 2012. Similarity of visual selectivity among clonally related neurons in visual cortex. Neuron 75(1):65-72. [PubMed: 22794261] [MGI Ref ID J:188353]
Paez-Gonzalez P; Abdi K; Luciano D; Liu Y; Soriano-Navarro M; Rawlins E; Bennett V; Garcia-Verdugo JM; Kuo CT. 2011. Ank3-Dependent SVZ Niche Assembly Is Required for the Continued Production of New Neurons. Neuron 71(1):61-75. [PubMed: 21745638] [MGI Ref ID J:174694]
Park D; Spencer JA; Koh BI; Kobayashi T; Fujisaki J; Clemens TL; Lin CP; Kronenberg HM; Scadden DT. 2012. Endogenous bone marrow MSCs are dynamic, fate-restricted participants in bone maintenance and regeneration. Cell Stem Cell 10(3):259-72. [PubMed: 22385654] [MGI Ref ID J:185671]
Ren H; Orozco IJ; Su Y; Suyama S; Gutierrez-Juarez R; Horvath TL; Wardlaw SL; Plum L; Arancio O; Accili D. 2012. FoxO1 target Gpr17 activates AgRP neurons to regulate food intake. Cell 149(6):1314-26. [PubMed: 22682251] [MGI Ref ID J:186157]
Sando R 3rd; Gounko N; Pieraut S; Liao L; Yates J 3rd; Maximov A. 2012. HDAC4 governs a transcriptional program essential for synaptic plasticity and memory. Cell 151(4):821-34. [PubMed: 23141539] [MGI Ref ID J:193206]
Sasaki M; Knobbe CB; Itsumi M; Elia AJ; Harris IS; Chio II; Cairns RA; McCracken S; Wakeham A; Haight J; Ten AY; Snow B; Ueda T; Inoue S; Yamamoto K; Ko M; Rao A; Yen KE; Su SM; Mak TW. 2012. D-2-hydroxyglutarate produced by mutant IDH1 perturbs collagen maturation and basement membrane function. Genes Dev 26(18):2038-49. [PubMed: 22925884] [MGI Ref ID J:187697]
Smith CL; Baek ST; Sung CY; Tallquist MD. 2011. Epicardial-derived cell epithelial-to-mesenchymal transition and fate specification require PDGF receptor signaling. Circ Res 108(12):e15-26. [PubMed: 21512159] [MGI Ref ID J:185693]
Song J; Xu Y; Hu X; Choi B; Tong Q. 2010. Brain expression of Cre recombinase driven by pancreas-specific promoters. Genesis 48(11):628-34. [PubMed: 20824628] [MGI Ref ID J:166612]
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. [PubMed: 21943598] [MGI Ref ID J:177261]
Taniguchi H; Lu J; Huang ZJ. 2013. The spatial and temporal origin of chandelier cells in mouse neocortex. Science 339(6115):70-4. [PubMed: 23180771] [MGI Ref ID J:190658]
Wang L; Budolfson K; Wang F. 2011. Pik3c3 deletion in pyramidal neurons results in loss of synapses, extensive gliosis and progressive neurodegeneration. Neuroscience 172:427-42. [PubMed: 20955765] [MGI Ref ID J:170162]
Xu Y; O'Brien WG 3rd; Lee CC; Myers MG Jr; Tong Q. 2012. Role of GABA release from leptin receptor-expressing neurons in body weight regulation. Endocrinology 153(5):2223-33. [PubMed: 22334723] [MGI Ref ID J:185007]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 10-SEP-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Gt(ROSA)26Sortm9(CAG-tdTomato)Hze x Homozygous for Gt(ROSA)26Sortm9(CAG-tdTomato)Hze Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Gt(ROSA)26Sortm9(CAG-tdTomato)Hze x Homozygous for Gt(ROSA)26Sortm9(CAG-tdTomato)Hze Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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