Strain Name: |
B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J |
|---|---|
Stock Number: |
007911 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(Thy1-kOFP,-mCherry,-mYFP,-mCerulean)M11Lich/J (Changed: 15-NOV-07
) |
| Genes & Alleles | CFP; OFP; RFP; Thy1; YFP; Tg(Thy1-Brainbow1.1)MLich; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Jeff Lichtman and Joshua Sanes, Harvard University Strain Description
View Brainbow images for Stock No's 007901, 007910, 007911, and 007921.
These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP), mYFP, and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the single FRT site inserted in the transgene allows tandem transgene copy number reduction through Flp-mediated recombination if desired. These Brainbow 1.1 (founder line M) mice allow mutually exclusive expression of three membrane-targeted fluorescent proteins in astrocytes throughout the brain, providing unambiguous delineation of boundaries between adjacent astrocytes in cre recombined cells, and may also be useful in conjunction with other Brainbow strains (Stock No. 007901, Stock No. 007910, Stock No. 007921) for neurobiological studies.Strain Development
The Thy1-Brainbow 1.1 transgene was designed with the mouse Thy1 regulatory elements surrounding four fluorescent proteins (orange (OFP), red (RFP), enhanced yellow (EYFP), and cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.1 coding region contained (from 5’ to 3’) a LoxN site, Lox2272 site, LoxP site, Kusabira-Orange OFP (kOFP; with polyA sequence), LoxN site, mCherry RFP (with membrane tethering palmitoylation sequence and polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5’ FRT site and polyA sequence). This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. Founder mice (line M) were bred with C57BL/6. The resulting mutant mice were backcrossed to C57BL/6 for at least 5 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.Kusabira-Orange (KO or kOFP) is a true OFP with an artificial amino acid sequence attached to the N-terminus of the protein allowing bright orange fluorescence in cells. The mCherry RFP is an optimized DsRed/mRFP1 fluorescent protein variant with a combination of amino acid substitutions designed to improve spectral properties. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization.
Gene & Allele Details
| Allele Symbol | Tg(Thy1-Brainbow1.1)MLich | ||
|---|---|---|---|
| Allele Name | transgene insertion M, Jeff W Lichtman | ||
| Common Name(s) | Tg(Thy1-kOFP,-mCherry,-mYFP,-mCerulean)MLich; Thy1-brainbow-1.1 line M; | ||
| Mutation Made By | Jeff Lichtman and Joshua Sanes, Harvard University | ||
| Strain of Origin | C57BL/6 x CBA | ||
| Expressed Gene | RFP, Red Fluorescent Protein, jelly fish | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Expressed Gene | OFP, Orange Fluorescent Protein, jelly fish | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The Thy1-Brainbow 1.1 transgene was designed with the mouse Thy1 regulatory elements surrounding four fluorescent proteins (orange (OFP), red (RFP), enhanced yellow (EYFP), and cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.1 coding region contained (from 5? to 3?) a LoxN site, Lox2272 site, LoxP site, Kusabira-Orange OFP (kOFP; with polyA sequence), LoxN site, mCherry RFP (with membrane tethering palmitoylation sequence and polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5? FRT site and polyA sequence).Kusabira-Orange (KO or kOFP) is a true OFP with an artificial amino acid sequence attached to the N-terminus of the protein allowing bright orange fluorescence in cells. The mCherry RFP is an optimized DsRed/mRFP1 fluorescent protein variant with a combination of amino acid substitutions designed to improve spectral properties. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP(ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. [J:125961] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to C57BL/6J inbred mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
006067 129-Gt(ROSA)26Sortm2Luo/J 006041 129-Gt(ROSA)26Sortm3Luo/J 006080 B6.129-Gt(ROSA)26Sortm2Luo/J 006075 B6.129-Gt(ROSA)26Sortm3Luo/J 005884 B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 007576 STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J 005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007610 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
CFP relatedNeurobiology Research
Cre-lox System (loxP-flanked Sequences)
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox-System (loxP-flanked Sequences: Test/Reporter)
Developmental Biology Research (Cre-lox system)
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
FLP-FRT System (FRT-flanked Sequences)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Genetics Research (Tissue/Cell Markers: astrocyte-specific marker)
Genetics Research (Tissue/Cell Markers: astrocytes)
Genetics Research (Tissue/Cell Markers: astrocytes, neurons)
Genetics Research (Tissue/Cell Markers: glial cells)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: neurons)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Neurobiology Research (astrocyte-specific marker)
Neurobiology Research (cell marker)
YFP relatedResearch Tools
Fluorescent Proteins
Fluorescent Proteins
References
Selected Reference(s)
Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. [PubMed: 17972876] [J:125961]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J |
| Stock Number: | 007911 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a separate commercial license from Harvard University.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form