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| These En-1lki (or En1lacZ) mutant mice harbor a β-galactosidase "knock-in" allele that abolishes endogenous gene function and may be useful for reporter protein expression in En1-expressing tissues in studying engrailed protein function in limb patterning along the dorso-ventral axis, spinal cord V1 interneuron development, embryonic mesencephalon and rhombomere 1 (cerebellum) development, as well as developing somites, and skin. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center Description
En-1lki (or En1lacZ) mutant mice harbor a β-galactosidase "knock-in" allele that also abolishes endogenous gene function. While heterozygotes are viable and fertile, homozygous mice die at birth (unless when maintained on a C57BL/6 genetic background). Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern almost identical to the wildtype gene. These En-1lacZ mice may be useful for reporter protein expression in En1-expressing tissues in studying engrailed protein function in limb patterning along the dorso-ventral axis, spinal cord V1 interneuron development, embryonic mesencephalon and rhombomere 1 (cerebellum) development, as well as developing somites, and skin.Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007916, Stock No. 007917, Stock No. 007918, Stock No. 007924, and Stock No. 007925).
Development
A targeting construct was designed to replace the first 111 amino acids of the targeted gene (ApaI-ClaI fragment) with a β-galactosidase (lacZ) coding sequence and loxP-flanked PGKNeo cassette; thus placing lacZ just downstream of the endogenous promoter and regulatory elements. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with morulae, and chimeric males were bred with CD1 females to generate mutant mice. Heterozygous mice were then bred to cre expressing mice to remove the floxed neo cassette. The resulting En-1lki (or En1lacZ) mice were maintained on a mixed genetic background (mostly outbred Swiss Webster but may be some other genetic background contributions) prior to arrival at The Jackson Laboratory. Upon arrival, En1lacZ mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (256 strains)
003343 B6.129S2-En1tm1Alj/J 002656 STOCK En1tm1Alj/J 007916 STOCK En1tm2(cre)Wrst/J 007917 STOCK En1tm7(cre/ESR1)Alj/J 007918 STOCK En1tm8.1Alj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
En1tm2Alj/En1+
involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
- nervous system phenotype
- *normal* nervous system phenotype
- cerebellum and colliculi have normal morphology at E18.5 (MGI Ref ID J:134516)
En1tm2Alj/En1tm2Alj
involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
- nervous system phenotype
- abnormal cerebellum morphology
- some lateral tissue is present at E18.5 (MGI Ref ID J:134516)
- abnormal cerebellum posterior vermis morphology (MGI Ref ID J:134516)
- abnormal midbrain development
- amount of tissue is reduced in mutants (MGI Ref ID J:134516)
- abnormal rhombomere morphology
- amount of rhombomere 1 tissue is reduced in mutants (MGI Ref ID J:134516)
- absent inferior colliculus
- structure does not form (MGI Ref ID J:134516)
- decreased superior colliculus size
- reduced in size; in some animals only small amount of tissue forms (MGI Ref ID J:134516)
- embryogenesis phenotype
- abnormal rhombomere morphology
- amount of rhombomere 1 tissue is reduced in mutants (MGI Ref ID J:134516)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Perinatal Lethality
Homozygous
Neurobiology Research
lacZ expression in neural tissue
Research Tools
lacZ Expression
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: multiple
Tissue/Cell Markers: neurons
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | En1tm2Alj | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Alexandra L Joyner | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | En-1Lki; En1lki; EnlacZ; | ||
| Mutation Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | LacZ expression is observed in a pattern almost identical to the wildtype gene and may be useful in studying limb patterning along the dorso-ventral axis, spinal cord V1 interneuron development, embryonic mesencephalon and rhombomere 1 (cerebellum) development, as well as developing somites, and skin. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | LacZ sequences were inserted downstream of the En1 promoter. The sequences corresponding to the first 111 amino acids of the reading frame are also deleted. [MGI Ref ID J:27767] | ||
| Gene Symbol and Name | En1, engrailed 1 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | En-1; Mo-en.1; engrailed-1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
En1tm2Alj SEP PCR, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hanks M; Wurst W; Anson-Cartwright L; Auerbach AB; Joyner AL. 1995. Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2 [see comments] Science 269(5224):679-82. [PubMed: 7624797] [MGI Ref ID J:27767]
Additional References
En1tm2Alj relatedAlberi L; Sgado P; Simon HH. 2004. Engrailed genes are cell-autonomously required to prevent apoptosis in mesencephalic dopaminergic neurons. Development 131(13):3229-36. [PubMed: 15175251] [MGI Ref ID J:98363]
Atit R; Sgaier SK; Mohamed OA; Taketo MM; Dufort D; Joyner AL; Niswander L; Conlon RA. 2006. Beta-catenin activation is necessary and sufficient to specify the dorsal dermal fate in the mouse. Dev Biol 296(1):164-76. [PubMed: 16730693] [MGI Ref ID J:119323]
Broccoli V; Boncinelli E; Wurst W. 1999. The caudal limit of Otx2 expression positions the isthmic organizer. Nature 401(6749):164-8. [PubMed: 10490025] [MGI Ref ID J:57538]
Deckelbaum RA; Holmes G; Zhao Z; Tong C; Basilico C; Loomis CA. 2012. Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1. Development 139(7):1346-58. [PubMed: 22395741] [MGI Ref ID J:184618]
Deckelbaum RA; Majithia A; Booker T; Henderson JE; Loomis CA. 2006. The homeoprotein engrailed 1 has pleiotropic functions in calvarial intramembranous bone formation and remodeling. Development 133(1):63-74. [PubMed: 16319118] [MGI Ref ID J:104802]
Kimmel RA; Turnbull DH; Blanquet V; Wurst W; Loomis CA; Joyner AL. 2000. Two lineage boundaries coordinate vertebrate apical ectodermal ridge formation. Genes Dev 14(11):1377-89. [PubMed: 10837030] [MGI Ref ID J:62572]
Liu A; Joyner AL. 2001. EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain region. Development 128(2):181-91. [PubMed: 11124114] [MGI Ref ID J:66592]
Loomis CA; Kimmel RA; Tong CX; Michaud J; Joyner AL. 1998. Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development 125(6):1137-48. [PubMed: 9463360] [MGI Ref ID J:46971]
Louvi A; Alexandre P; Metin C; Wurst W; Wassef M. 2003. The isthmic neuroepithelium is essential for cerebellar midline fusion. Development 130(22):5319-30. [PubMed: 14507778] [MGI Ref ID J:85727]
Matise MP; Joyner AL. 1997. Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci 17(20):7805-16. [PubMed: 9315901] [MGI Ref ID J:43464]
Orvis GD; Hartzell AL; Smith JB; Barraza LH; Wilson SL; Szulc KU; Turnbull DH; Joyner AL. 2012. The engrailed homeobox genes are required in multiple cell lineages to coordinate sequential formation of fissures and growth of the cerebellum. Dev Biol 367(1):25-39. [PubMed: 22564796] [MGI Ref ID J:185802]
Sgaier SK; Lao Z; Villanueva MP; Berenshteyn F; Stephen D; Turnbull RK; Joyner AL. 2007. Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins. Development 134(12):2325-35. [PubMed: 17537797] [MGI Ref ID J:134516]
Sonnier L; Le Pen G; Hartmann A; Bizot JC; Trovero F; Krebs MO; Prochiantz A. 2007. Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1. J Neurosci 27(5):1063-71. [PubMed: 17267560] [MGI Ref ID J:117895]
Tran TH; Jarrell A; Zentner GE; Welsh A; Brownell I; Scacheri PC; Atit R. 2010. Role of canonical Wnt signaling/ss-catenin via Dermo1 in cranial dermal cell development. Development 137(23):3973-84. [PubMed: 20980404] [MGI Ref ID J:166907]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype siblings. Homozygotes die at birth.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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