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| When these Gli1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Gli1 expressing cells; making them useful for studying axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names STOCK Gli1tm3(cre/ESR1)Alj/J (Changed: 17-MAR-10 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x +/+ sibling (Female x Male) 17-FEB-11 Species laboratory mouse Generation F?+F8 (19-OCT-12)
Generation DefinitionsDonating Investigator Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center Description
Mice homozygous for this Gli1-CreERT2 targeted allele are viable and fertile (although homozygous males are reported to have breeding problems). Under control of the endogenous upstream promoter/enhancer elements, tamoxifen-inducible cre activity is observed in cells that have received positive Hedgehog/Sonic Hedgehog signaling. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Gli1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Gli1 expressing cells; making them useful for studying axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.Development
A targeting vector was designed with a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain) followed by three tandem SV40 polyA sequence repeats and a reverse-oriented loxP-flanked neo cassette. This construct was inserted into the targeted gene (replacing a 2.3 kb region including the ATG site in exon 2 and first zinc finger domain in exon 4) via electroporation into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred to either Black-Swiss or C57BL/6J mice. The Gli1-CreERT2-neo heterozygotes were then bred to cre expressing mice (Black-Swiss genetic background) to remove the neo cassette. The resulting Gli1-CreERT2 offspring (with a single loxP site remaining after the SV40 polyA sequences) were bred to Swiss Webster outbred mice for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J mice for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gli1
008211 STOCK Gli1tm2Alj/J View Strains carrying other alleles of Gli1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gli1tm3(cre/ERT2)Alj | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Alexandra L Joyner | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gli1-CreERT2; Gli1CreER; Gli1CreERT2; Gli1tm3(cre/ERT2)Alj; Gli1tm3(cre/ESR1)Alj; | ||
| Mutation Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | tamoxifen-inducible cre activity is observed in cells that have received positive Hedgehog/Sonic Hedgehog signaling | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Gli1 | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | CreERT2 cDNA and a floxed neo were inserted into the 5' UTR of the first coding exon. Crossing with mice expressing Cre removed the floxed neo. Cre expression in mice carrying this allele is induced by treating the mice with tamoxifen. [MGI Ref ID J:92507] | ||
| Gene Symbol and Name | Gli1, GLI-Kruppel family member GLI1 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | AV235269; GLI; Zfp-5; Zfp5; expressed sequence AV235269; zinc finger protein 5; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Gli1tm3(cre/ERT2)Aljalternate3,SEPARATED MELT
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ahn S; Joyner AL. 2004. Dynamic changes in the response of cells to positive hedgehog signaling during mouse limb patterning. Cell 118(4):505-16. [PubMed: 15315762] [MGI Ref ID J:92507]
Additional References
Gli1tm3(cre/ERT2)Alj relatedAoto K; Shikata Y; Imai H; Matsumaru D; Tokunaga T; Shioda S; Yamada G; Motoyama J. 2009. Mouse Shh is required for prechordal plate maintenance during brain and craniofacial morphogenesis. Dev Biol 327(1):106-20. [PubMed: 19103193] [MGI Ref ID J:145732]
Bouldin CM; Gritli-Linde A; Ahn S; Harfe BD. 2010. Shh pathway activation is present and required within the vertebrate limb bud apical ectodermal ridge for normal autopod patterning. Proc Natl Acad Sci U S A 107(12):5489-94. [PubMed: 20212115] [MGI Ref ID J:158689]
Carney RS; Mangin JM; Hayes L; Mansfield K; Sousa VH; Fishell G; Machold RP; Ahn S; Gallo V; Corbin JG. 2010. Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala. Neural Dev 5:14. [PubMed: 20507551] [MGI Ref ID J:161400]
Deckelbaum RA; Holmes G; Zhao Z; Tong C; Basilico C; Loomis CA. 2012. Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1. Development 139(7):1346-58. [PubMed: 22395741] [MGI Ref ID J:184618]
Friedland-Little JM; Hoffmann AD; Ocbina PJ; Peterson MA; Bosman JD; Chen Y; Cheng SY; Anderson KV; Moskowitz IP. 2011. A novel murine allele of Intraflagellar Transport Protein 172 causes a syndrome including VACTERL-like features with hydrocephalus. Hum Mol Genet 20(19):3725-37. [PubMed: 21653639] [MGI Ref ID J:175371]
Garcia AD; Petrova R; Eng L; Joyner AL. 2010. Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain. J Neurosci 30(41):13597-608. [PubMed: 20943901] [MGI Ref ID J:165495]
Haraguchi R; Matsumaru D; Nakagata N; Miyagawa S; Suzuki K; Kitazawa S; Yamada G. 2012. The hedgehog signal induced modulation of bone morphogenetic protein signaling: an essential signaling relay for urinary tract morphogenesis. PLoS One 7(7):e42245. [PubMed: 22860096] [MGI Ref ID J:189675]
Haraguchi R; Motoyama J; Sasaki H; Satoh Y; Miyagawa S; Nakagata N; Moon A; Yamada G. 2007. Molecular analysis of coordinated bladder and urogenital organ formation by Hedgehog signaling. Development 134(3):525-33. [PubMed: 17202190] [MGI Ref ID J:119913]
Hayes L; Ralls S; Wang H; Ahn S. 2013. Duration of Shh signaling contributes to mDA neuron diversity. Dev Biol 374(1):115-26. [PubMed: 23201023] [MGI Ref ID J:193109]
Hoffmann AD; Peterson MA; Friedland-Little JM; Anderson SA; Moskowitz IP. 2009. sonic hedgehog is required in pulmonary endoderm for atrial septation. Development 136(10):1761-70. [PubMed: 19369393] [MGI Ref ID J:148020]
Huang CC; Miyagawa S; Matsumaru D; Parker KL; Yao HH. 2010. Progenitor cell expansion and organ size of mouse adrenal is regulated by sonic hedgehog. Endocrinology 151(3):1119-28. [PubMed: 20118198] [MGI Ref ID J:168522]
Huang X; Ketova T; Fleming JT; Wang H; Dey SK; Litingtung Y; Chiang C. 2009. Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus. Development 136(15):2535-43. [PubMed: 19570847] [MGI Ref ID J:152851]
Ihrie RA; Shah JK; Harwell CC; Levine JH; Guinto CD; Lezameta M; Kriegstein AR; Alvarez-Buylla A. 2011. Persistent sonic hedgehog signaling in adult brain determines neural stem cell positional identity. Neuron 71(2):250-62. [PubMed: 21791285] [MGI Ref ID J:174685]
King P; Paul A; Laufer E. 2009. Shh signaling regulates adrenocortical development and identifies progenitors of steroidogenic lineages. Proc Natl Acad Sci U S A 106(50):21185-90. [PubMed: 19955443] [MGI Ref ID J:155826]
Li CY; Cha W; Luder HU; Charles RP; McMahon M; Mitsiadis TA; Klein OD. 2012. E-cadherin regulates the behavior and fate of epithelial stem cells and their progeny in the mouse incisor. Dev Biol 366(2):357-66. [PubMed: 22537490] [MGI Ref ID J:185423]
Li G; Kataoka H; Coughlin SR; Pleasure SJ. 2009. Identification of a transient subpial neurogenic zone in the developing dentate gyrus and its regulation by Cxcl12 and reelin signaling. Development 136(2):327-35. [PubMed: 19103804] [MGI Ref ID J:143512]
Mao J; Kim BM; Rajurkar M; Shivdasani RA; McMahon AP. 2010. Hedgehog signaling controls mesenchymal growth in the developing mammalian digestive tract. Development 137(10):1721-9. [PubMed: 20430747] [MGI Ref ID J:160363]
Matsumaru D; Haraguchi R; Miyagawa S; Motoyama J; Nakagata N; Meijlink F; Yamada G. 2011. Genetic analysis of Hedgehog signaling in ventral body wall development and the onset of omphalocele formation. PLoS One 6(1):e16260. [PubMed: 21283718] [MGI Ref ID J:169568]
Miyagawa S; Satoh Y; Haraguchi R; Suzuki K; Iguchi T; Taketo MM; Nakagata N; Matsumoto T; Takeyama KI; Kato S; Yamada G. 2009. Genetic interactions of the Androgen and Wnt/{beta}-catenin Pathways for the Masculinization of External Genitalia. Mol Endocrinol 23(6):871-880. [PubMed: 19282366] [MGI Ref ID J:148515]
Schuller U; Heine VM; Mao J; Kho AT; Dillon AK; Han YG; Huillard E; Sun T; Ligon AH; Qian Y; Ma Q; Alvarez-Buylla A; McMahon AP; Rowitch DH; Ligon KL. 2008. Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma. Cancer Cell 14(2):123-34. [PubMed: 18691547] [MGI Ref ID J:139574]
Seidel K; Ahn CP; Lyons D; Nee A; Ting K; Brownell I; Cao T; Carano RA; Curran T; Schober M; Fuchs E; Joyner A; Martin GR; de Sauvage FJ; Klein OD. 2010. Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor. Development 137(22):3753-61. [PubMed: 20978073] [MGI Ref ID J:167067]
Shikata Y; Okada T; Hashimoto M; Ellis T; Matsumaru D; Shiroishi T; Ogawa M; Wainwright B; Motoyama J. 2011. Ptch1-mediated dosage-dependent action of Shh signaling regulates neural progenitor development at late gestational stages. Dev Biol 349(2):147-59. [PubMed: 20969845] [MGI Ref ID J:168035]
Shin K; Lee J; Guo N; Kim J; Lim A; Qu L; Mysorekar IU; Beachy PA. 2011. Hedgehog/Wnt feedback supports regenerative proliferation of epithelial stem cells in bladder. Nature 472(7341):110-4. [PubMed: 21389986] [MGI Ref ID J:170991]
Thomas NA; Koudijs M; van Eeden FJ; Joyner AL; Yelon D. 2008. Hedgehog signaling plays a cell-autonomous role in maximizing cardiac developmental potential. Development 135(22):3789-99. [PubMed: 18842815] [MGI Ref ID J:143414]
Xie L; Hoffmann AD; Burnicka-Turek O; Friedland-Little JM; Zhang K; Moskowitz IP. 2012. Tbx5-hedgehog molecular networks are essential in the second heart field for atrial septation. Dev Cell 23(2):280-91. [PubMed: 22898775] [MGI Ref ID J:187715]
Yu W; McDonnell K; Taketo MM; Bai CB. 2008. Wnt signaling determines ventral spinal cord cell fates in a time-dependent manner. Development 135(22):3687-96. [PubMed: 18927156] [MGI Ref ID J:143585]
Yu W; Wang Y; McDonnell K; Stephen D; Bai CB. 2009. Patterning of ventral telencephalon requires positive function of Gli transcription factors. Dev Biol 334(1):264-75. [PubMed: 19632216] [MGI Ref ID J:153550]
Zervas M; Millet S; Ahn S; Joyner AL. 2004. Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1. Neuron 43(3):345-57. [PubMed: 15294143] [MGI Ref ID J:152670]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice mice may be bred together or to wildtype mice from the colony. The donating investigator reports that homozygous males do not breed well. Mating System Heterozygote x +/+ sibling (Female x Male) 17-FEB-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Gli1tm3(cre/ESR1)Alj
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Gli1tm3(cre/ESR1)Alj x Wild-type for Gli1tm3(cre/ESR1)Alj $296.00 Wild-type for Gli1tm3(cre/ESR1)Alj x Heterozygous for Gli1tm3(cre/ESR1)Alj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Gli1tm3(cre/ESR1)Alj
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Gli1tm3(cre/ESR1)Alj x Wild-type for Gli1tm3(cre/ESR1)Alj $384.80 Wild-type for Gli1tm3(cre/ESR1)Alj x Heterozygous for Gli1tm3(cre/ESR1)Alj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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