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| These Amh-cre transgenic mice have expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis. | |||||||||||
Type Congenic; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Robert Braun, The Jackson Laboratory Description
Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis.Development
The Amh-cre transgene was designed with an anti-Mullerian hormone (Amh) genomic promoter fragment (containing the entire promoter region from -634 to the AUG start site), a Myc-tagged Cre recombinase cDNA (inserted such that the cre AUG replaced the Amh AUG), an hGH polyA signal, and the Amh intron 1. The transgene was microinjected into the pronucleus of FVB/N zygotes. Mice from founder line 8815 were found to have testis Sertoli cell-specific cre activity and were subsequently backcrossed to 129/SvJaeSor (129S4/SvJaeSor) for at least 11 generations. Mice were subsequently rederived using 129S1/SvImJ inbred mice (Stock No. 002448) when Dr. Robert Braun moved his research colony to The Jackson Laboratory. To transfer these mice to The Jackson Laboratory Repository, the Amh-cre transgenic mice were again rederived using 129S1/SvImJ inbred mice (Stock No. 002448) to establish this distribution colony.
| Control | ||
|---|---|---|
| Noncarrier | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Amh
002188 B6.129S7-Amhtm1Bhr/J 002187 B6;129S7-Amhtm1Bhr/J View Strains carrying other alleles of Amh (2 strains)
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (125 strains)
Congenic Nomenclature
Cre-lox Systems
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
cre relatedReproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: spermatogonial transplantation marker)
Genetics Research (Tissue/Cell Markers: testicular cell marker)
Reproductive Biology Research (Cre-lox System)
Reproductive Biology Research (male germ cells)
Reproductive Biology Research (spermatogonial transplantation marker)
Reproductive Biology Research (testicular cell marker)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
| Allele Symbol | Tg(Amh-cre)8815Reb | ||
|---|---|---|---|
| Allele Name | transgene insertion 8815, Robert E Braun | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Mutation Made By | Robert Braun, The Jackson Laboratory | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Cre is expressed specifically in testis Sertoli cells. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Amh, anti-Mullerian hormone, mouse, laboratory | ||
| Molecular Note | The promoter region of the Amh gene was fused, at the AUG start codon of exon 1, to a Cre cDNA. Intron 1 of the Amh gene was left fused to the 3' end of Cre. [MGI Ref ID J:90393] | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
Holdcraft RW; Braun RE. 2004. Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids. Development 131(2):459-67. [PubMed: 14701682] [MGI Ref ID J:90393]
Tg(Amh-cre)8815Reb relatedMeng J; Holdcraft RW; Shima JE; Griswold MD; Braun RE. 2005. Androgens regulate the permeability of the blood-testis barrier. Proc Natl Acad Sci U S A 102(46):16696-700. [PubMed: 16275920] [MGI Ref ID J:103744]
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together or to wildtype (noncarrier) siblings. As Amh-cre transgene expression is specific to the Sertoli cells of the testis, transmission of the transgene must be through the male germline for experiments involving cre-induced recombination.
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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| Control | ||
|---|---|---|
| Noncarrier | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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