| |||||||||||||||
| These Amh-cre transgenic mice have expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 21-NOV-08 Species laboratory mouse Generation N13+F1 (23-MAR-09) Donating Investigator Robert Braun, The Jackson Laboratory Description
Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis.Development
The Amh-cre transgene was designed with an anti-Mullerian hormone (Amh) genomic promoter fragment (containing the entire promoter region from -634 to the AUG start site), a Myc-tagged Cre recombinase cDNA (inserted such that the cre AUG replaced the Amh AUG), an hGH polyA signal, and the Amh intron 1. The transgene was microinjected into the pronucleus of FVB/N zygotes. Mice from founder line 8815 were found to have testis Sertoli cell-specific cre activity and were subsequently backcrossed to 129/SvJaeSor (129S4/SvJaeSor) for at least 11 generations. Mice were subsequently rederived using 129S1/SvImJ inbred mice (Stock No. 002448) when Dr. Robert Braun moved his research colony to The Jackson Laboratory. To transfer these mice to The Jackson Laboratory Repository, the Amh-cre transgenic mice were again rederived using 129S1/SvImJ inbred mice (Stock No. 002448) to establish this distribution colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Amh
002188 B6.129S7-Amhtm1Bhr/J 002187 B6;129S7-Amhtm1Bhr/J View Strains carrying other alleles of Amh (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedReproductive Biology Research
Developmental Defects Affecting Gonads
males only
Fertility Defects
males only
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: spermatogonial transplantation marker
Tissue/Cell Markers: testicular cell marker
Reproductive Biology Research
Cre-lox System
male germ cells
spermatogonial transplantation marker
testicular cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Amh-cre)8815Reb | ||
|---|---|---|---|
| Allele Name | transgene insertion 8815, Robert E Braun | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Mutation Made By | Robert Braun, The Jackson Laboratory | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Cre is expressed specifically in testis Sertoli cells. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Amh, anti-Mullerian hormone, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Amh-cre)8815Reb, transgene insertion 8815, Robert E Braun | ||
| Chromosome | UN | ||
| Driver Note | Amh | ||
| Molecular Note | The promoter region of the Amh gene was fused, at the AUG start codon of exon 1, to a Cre cDNA. Intron 1 of the Amh gene was left fused to the 3' end of Cre. [MGI Ref ID J:90393] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Holdcraft RW; Braun RE. 2004. Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids. Development 131(2):459-67. [PubMed: 14701682] [MGI Ref ID J:90393]
Additional References
Tg(Amh-cre)8815Reb relatedMeng J; Holdcraft RW; Shima JE; Griswold MD; Braun RE. 2005. Androgens regulate the permeability of the blood-testis barrier. Proc Natl Acad Sci U S A 102(46):16696-700. [PubMed: 16275920] [MGI Ref ID J:103744]
Nalam RL; Andreu-Vieyra C; Braun RE; Akiyama H; Matzuk MM. 2009. Retinoblastoma protein plays multiple essential roles in the terminal differentiation of Sertoli cells. Mol Endocrinol 23(11):1900-13. [PubMed: 19819985] [MGI Ref ID J:154066]
Sanz E; Yang L; Su T; Morris DR; McKnight GS; Amieux PS. 2009. Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Proc Natl Acad Sci U S A 106(33):13939-44. [PubMed: 19666516] [MGI Ref ID J:151962]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together or to wildtype (noncarrier) siblings. As Amh-cre transgene expression is specific to the Sertoli cells of the testis, transmission of the transgene must be through the male germline for experiments involving cre-induced recombination. Mating System Noncarrier x Hemizygote (Female x Male) 21-NOV-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Amh-cre)8815Reb
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Amh-cre)8815Reb x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Amh-cre)8815Reb
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Amh-cre)8815Reb
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Amh-cre)8815Reb x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Amh-cre)8815Reb
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.