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| These En1Cki (or En1Cre) mutant mice harbor a Cre recombinase (cre) "knock-in" allele that also abolishes endogenous gene function and may be useful for Cre-lox applications studying engrailed protein function such as deleting genes in spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK En1tm2(cre)Alj/J (Changed: 02-FEB-09 ) STOCK En1tm6(cre)Alj/J (Changed: 29-JAN-09 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center Description
En1Cki (or En1Cre) mutant mice harbor a Cre recombinase (cre) "knock-in" allele that also abolishes endogenous gene function. While heterozygotes are viable and fertile, homozygous mice die at birth. Under control of the upstream promoter/enhancer elements, cre expression is observed in a pattern consistent with the wild-type gene. When heterozygotes are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in En1-expressing tissues of the offspring. These En1Cki (or En1Cre) mice may be useful for Cre-lox applications studying engrailed protein function such as deleting genes in spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues.Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007917, Stock No. 007918, Stock No. 007924, and Stock No. 007925).
Development
These mutant mice were originally made by Dr. Wolfgang Wurst (Helmholtz Centre, Munich, Germany) and then transferred to Dr. Alexandra L. Joyner (Skirball Institute, New York University School of Medicine). To generate the mutant allele, a targeting construct was designed to replace the first 111 amino acids of the targeted gene (ApaI-ClaI fragment) with a Cre recombinase (cre) gene and loxP-flanked PGKNeo cassette; thus placing cre just downstream of the endogenous promoter and regulatory elements. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells and chimeric mice were generated. After germline transmission, mutant mice were bred to CMV-Cre mice to remove the PGKNeo cassette (leaving a single loxP site remaining). The resulting En1Cki (or En1Cre) offspring were selectively bred to remove the CMV-Cre transgene and further maintained on a mixed genetic background (mostly outbred Swiss Webster but may be some other genetic background contributions) prior to arrival at The Jackson Laboratory. Upon arrival, En1Cki mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of En1
003343 B6.129S2-En1tm1Alj/J 002656 STOCK En1tm1Alj/J 007912 STOCK En1tm2Alj/J 007917 STOCK En1tm7(cre/ESR1)Alj/J 007918 STOCK En1tm8.1Alj/J View Strains carrying other alleles of En1 (5 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Perinatal Lethality
Homozygous
Neurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | En1tm2(cre)Wrst | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Wolfgang Wurst | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | En1-Cre; En1Cki; En1Cre; En1tm6(cre)Alj; | ||
| Mutation Made By | Wolfgang Wurst, HelmholtzZentrum münchen | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | En1 | ||
| Molecular Note | A cre recombinase gene cassette was inserted into the En1 locus. A loxP flanked neomycin cassette is removed in vivo via a Cre-mediated recombination event. [MGI Ref ID J:62572] | ||
| Gene Symbol and Name | En1, engrailed 1 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | En-1; Mo-en.1; engrailed-1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
En1tm2(cre)Wrst, Separated PCR
En1tm2(cre)Wrst, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kimmel RA; Turnbull DH; Blanquet V; Wurst W; Loomis CA; Joyner AL. 2000. Two lineage boundaries coordinate vertebrate apical ectodermal ridge formation. Genes Dev 14(11):1377-89. [PubMed: 10837030] [MGI Ref ID J:62572]
Additional References
En1tm2(cre)Wrst relatedAchim K; Peltopuro P; Lahti L; Li J; Salminen M; Partanen J. 2012. Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region. Development 139(13):2360-70. [PubMed: 22627282] [MGI Ref ID J:185533]
Ahmed I; Liang Y; Schools S; Dawson VL; Dawson TM; Savitt JM. 2012. Development and characterization of a new Parkinson's disease model resulting from impaired autophagy. J Neurosci 32(46):16503-9. [PubMed: 23152632] [MGI Ref ID J:192452]
Anzalone A; Lizardi-Ortiz JE; Ramos M; De Mei C; Hopf FW; Iaccarino C; Halbout B; Jacobsen J; Kinoshita C; Welter M; Caron MG; Bonci A; Sulzer D; Borrelli E. 2012. Dual Control of Dopamine Synthesis and Release by Presynaptic and Postsynaptic Dopamine D2 Receptors. J Neurosci 32(26):9023-9034. [PubMed: 22745501] [MGI Ref ID J:185643]
Atit R; Sgaier SK; Mohamed OA; Taketo MM; Dufort D; Joyner AL; Niswander L; Conlon RA. 2006. Beta-catenin activation is necessary and sufficient to specify the dorsal dermal fate in the mouse. Dev Biol 296(1):164-76. [PubMed: 16730693] [MGI Ref ID J:119323]
Bang SJ; Jensen P; Dymecki SM; Commons KG. 2012. Projections and interconnections of genetically defined serotonin neurons in mice. Eur J Neurosci 35(1):85-96. [PubMed: 22151329] [MGI Ref ID J:184339]
Basson MA; Echevarria D; Petersen Ahn C; Sudarov A; Joyner AL; Mason IJ; Martinez S; Martin GR. 2008. Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during development. Development 135(5):889-98. [PubMed: 18216176] [MGI Ref ID J:131764]
Betley JN; Wright CV; Kawaguchi Y; Erdelyi F; Szabo G; Jessell TM; Kaltschmidt JA. 2009. Stringent specificity in the construction of a GABAergic presynaptic inhibitory circuit. Cell 139(1):161-74. [PubMed: 19804761] [MGI Ref ID J:157313]
Blaess S; Corrales JD; Joyner AL. 2006. Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain region. Development 133(9):1799-809. [PubMed: 16571630] [MGI Ref ID J:108509]
Blaess S; Stephen D; Joyner AL. 2008. Gli3 coordinates three-dimensional patterning and growth of the tectum and cerebellum by integrating Shh and Fgf8 signaling. Development 135(12):2093-103. [PubMed: 18480159] [MGI Ref ID J:137136]
Blak AA; Naserke T; Saarimaki-Vire J; Peltopuro P; Giraldo-Velasquez M; Vogt Weisenhorn DM; Prakash N; Sendtner M; Partanen J; Wurst W. 2007. Fgfr2 and Fgfr3 are not required for patterning and maintenance of the midbrain and anterior hindbrain. Dev Biol 303(1):231-43. [PubMed: 17150206] [MGI Ref ID J:118802]
Borgkvist A; Puelles E; Carta M; Acampora D; Ang SL; Wurst W; Goiny M; Fisone G; Simeone A; Usiello A. 2006. Altered dopaminergic innervation and amphetamine response in adult Otx2 conditional mutant mice. Mol Cell Neurosci 31(2):293-302. [PubMed: 16256364] [MGI Ref ID J:106889]
Brown A; Brown S; Ellisor D; Hagan N; Normand E; Zervas M. 2009. A practical approach to genetic inducible fate mapping: a visual guide to mark and track cells in vivo. J Vis Exp (34):. [PubMed: 20042997] [MGI Ref ID J:162056]
Chen D; Jarrell A; Guo C; Lang R; Atit R. 2012. Dermal beta-catenin activity in response to epidermal Wnt ligands is required for fibroblast proliferation and hair follicle initiation. Development 139(8):1522-33. [PubMed: 22434869] [MGI Ref ID J:183485]
Chen H; McCaffery JM; Chan DC. 2007. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell 130(3):548-62. [PubMed: 17693261] [MGI Ref ID J:132329]
Chi CL; Martinez S; Wurst W; Martin GR. 2003. The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development 130(12):2633-44. [PubMed: 12736208] [MGI Ref ID J:83255]
Chilov D; Sinjushina N; Rita H; Taketo MM; Makela TP; Partanen J. 2011. Phosphorylated beta-catenin localizes to centrosomes of neuronal progenitors and is required for cell polarity and neurogenesis in developing midbrain. Dev Biol 357(1):259-68. [PubMed: 21736876] [MGI Ref ID J:175551]
Chilov D; Sinjushina N; Saarimaki-Vire J; Taketo MM; Partanen J. 2010. beta-Catenin regulates intercellular signalling networks and cell-type specific transcription in the developing mouse midbrain-rhombomere 1 region. PLoS One 5(6):e10881. [PubMed: 20532162] [MGI Ref ID J:161813]
Crespo-Enriquez I; Partanen J; Martinez S; Echevarria D. 2012. Fgf8-related secondary organizers exert different polarizing planar instructions along the mouse anterior neural tube. PLoS One 7(7):e39977. [PubMed: 22792203] [MGI Ref ID J:189513]
Dhande OS; Bhatt S; Anishchenko A; Elstrott J; Iwasato T; Swindell EC; Xu HP; Jamrich M; Itohara S; Feller MB; Crair MC. 2012. Role of adenylate cyclase 1 in retinofugal map development. J Comp Neurol 520(7):1562-83. [PubMed: 22102330] [MGI Ref ID J:187342]
Di Giovannantonio LG; Di Salvio M; Acampora D; Prakash N; Wurst W; Simeone A. 2013. Otx2 selectively controls the neurogenesis of specific neuronal subtypes of the ventral tegmental area and compensates En1-dependent neuronal loss and MPTP vulnerability. Dev Biol 373(1):176-83. [PubMed: 23117062] [MGI Ref ID J:192282]
Di Salvio M; Di Giovannantonio LG; Acampora D; Prosperi R; Omodei D; Prakash N; Wurst W; Simeone A. 2010. Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat Neurosci 13(12):1481-8. [PubMed: 21057506] [MGI Ref ID J:166896]
Ellisor D; Koveal D; Hagan N; Brown A; Zervas M. 2009. Comparative analysis of conditional reporter alleles in the developing embryo and embryonic nervous system. Gene Expr Patterns 9(7):475-89. [PubMed: 19616131] [MGI Ref ID J:152409]
Ellisor D; Rieser C; Voelcker B; Machan JT; Zervas M. 2012. Genetic dissection of midbrain dopamine neuron development in vivo. Dev Biol 372(2):249-62. [PubMed: 23041116] [MGI Ref ID J:190919]
Fernandes M; Antoine M; Hebert JM. 2012. SMAD4 is essential for generating subtypes of neurons during cerebellar development. Dev Biol 365(1):82-90. [PubMed: 22370000] [MGI Ref ID J:184924]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Goodnough LH; Chang AT; Treloar C; Yang J; Scacheri PC; Atit RP. 2012. Twist1 mediates repression of chondrogenesis by beta-catenin to promote cranial bone progenitor specification. Development 139(23):4428-38. [PubMed: 23095887] [MGI Ref ID J:189063]
Ishimura R; Martin GR; Ackerman SL. 2008. Loss of apoptosis-inducing factor results in cell-type-specific neurogenesis defects. J Neurosci 28(19):4938-48. [PubMed: 18463247] [MGI Ref ID J:135191]
Jensen P; Farago AF; Awatramani RB; Scott MM; Deneris ES; Dymecki SM. 2008. Redefining the serotonergic system by genetic lineage. Nat Neurosci 11(4):417-9. [PubMed: 18344997] [MGI Ref ID J:136096]
Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J. 2006. FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Dev Biol 297(1):141-57. [PubMed: 16782087] [MGI Ref ID J:112633]
Kala K; Haugas M; Lillevali K; Guimera J; Wurst W; Salminen M; Partanen J. 2009. Gata2 is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons. Development 136(2):253-62. [PubMed: 19088086] [MGI Ref ID J:143527]
Kim JC; Cook MN; Carey MR; Shen C; Regehr WG; Dymecki SM. 2009. Linking genetically defined neurons to behavior through a broadly applicable silencing allele. Neuron 63(3):305-15. [PubMed: 19679071] [MGI Ref ID J:154944]
Lahti L; Peltopuro P; Piepponen TP; Partanen J. 2012. Cell-autonomous FGF signaling regulates anteroposterior patterning and neuronal differentiation in the mesodiencephalic dopaminergic progenitor domain. Development 139(5):894-905. [PubMed: 22278924] [MGI Ref ID J:182759]
Lahti L; Saarimaki-Vire J; Rita H; Partanen J. 2011. FGF signaling gradient maintains symmetrical proliferative divisions of midbrain neuronal progenitors. Dev Biol 349(2):270-82. [PubMed: 21074523] [MGI Ref ID J:168023]
Li JY; Lao Z; Joyner AL. 2002. Changing requirements for Gbx2 in development of the cerebellum and maintenance of the mid/hindbrain organizer. Neuron 36(1):31-43. [PubMed: 12367504] [MGI Ref ID J:79431]
Machold R; Fishell G. 2005. Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors. Neuron 48(1):17-24. [PubMed: 16202705] [MGI Ref ID J:105186]
Machold RP; Kittell DJ; Fishell GJ. 2007. Antagonism between Notch and bone morphogenetic protein receptor signaling regulates neurogenesis in the cerebellar rhombic lip. Neural Dev 2:5. [PubMed: 17319963] [MGI Ref ID J:160654]
Ming Kwan K; Li AG; Wang XJ; Wurst W; Behringer RR. 2004. Essential roles of BMPR-IA signaling in differentiation and growth of hair follicles and in skin tumorigenesis. Genesis 39(1):10-25. [PubMed: 15124223] [MGI Ref ID J:89759]
Mourikis P; Gopalakrishnan S; Sambasivan R; Tajbakhsh S. 2012. Cell-autonomous Notch activity maintains the temporal specification potential of skeletal muscle stem cells. Development 139(24):4536-48. [PubMed: 23136394] [MGI Ref ID J:189959]
Ohtola J; Myers J; Akhtar-Zaidi B; Zuzindlak D; Sandesara P; Yeh K; Mackem S; Atit R. 2008. {beta}-Catenin has sequential roles in the survival and specification of ventral dermis. Development 135(13):2321-9. [PubMed: 18539925] [MGI Ref ID J:137352]
Okano J; Levy C; Lichti U; Sun HW; Yuspa SH; Sakai Y; Morasso MI. 2012. Cutaneous retinoic acid levels determine hair follicle development and downgrowth. J Biol Chem 287(47):39304-15. [PubMed: 23007396] [MGI Ref ID J:192641]
Omodei D; Acampora D; Mancuso P; Prakash N; Di Giovannantonio LG; Wurst W; Simeone A. 2008. Anterior-posterior graded response to Otx2 controls proliferation and differentiation of dopaminergic progenitors in the ventral mesencephalon. Development 135(20):3459-70. [PubMed: 18820178] [MGI Ref ID J:143341]
Peng C; Li N; Ng YK; Zhang J; Meier F; Theis FJ; Merkenschlager M; Chen W; Wurst W; Prakash N. 2012. A Unilateral Negative Feedback Loop Between miR-200 microRNAs and Sox2/E2F3 Controls Neural Progenitor Cell-Cycle Exit and Differentiation. J Neurosci 32(38):13292-13308. [PubMed: 22993445] [MGI Ref ID J:188279]
Perez-Balaguer A; Puelles E; Wurst W; Martinez S. 2009. Shh dependent and independent maintenance of basal midbrain. Mech Dev 126(5-6):301-13. [PubMed: 19298856] [MGI Ref ID J:149237]
Prakash N; Brodski C; Naserke T; Puelles E; Gogoi R; Hall A; Panhuysen M; Echevarria D; Sussel L; Weisenhorn DM; Martinez S; Arenas E; Simeone A; Wurst W. 2006. A Wnt1-regulated genetic network controls the identity and fate of midbrain-dopaminergic progenitors in vivo. Development 133(1):89-98. [PubMed: 16339193] [MGI Ref ID J:104370]
Prakash N; Puelles E; Freude K; Trumbach D; Omodei D; Di Salvio M; Sussel L; Ericson J; Sander M; Simeone A; Wurst W. 2009. Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain. Development 136(15):2545-55. [PubMed: 19592574] [MGI Ref ID J:152847]
Puelles E; Annino A; Tuorto F; Usiello A; Acampora D; Czerny T; Brodski C; Ang SL; Wurst W; Simeone A. 2004. Otx2 regulates the extent, identity and fate of neuronal progenitor domains in the ventral midbrain. Development 131(9):2037-48. [PubMed: 15105370] [MGI Ref ID J:89367]
Rinkevich Y; Lindau P; Ueno H; Longaker MT; Weissman IL. 2011. Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip. Nature 476(7361):409-13. [PubMed: 21866153] [MGI Ref ID J:188630]
Sgaier SK; Lao Z; Villanueva MP; Berenshteyn F; Stephen D; Turnbull RK; Joyner AL. 2007. Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins. Development 134(12):2325-35. [PubMed: 17537797] [MGI Ref ID J:134516]
Sgaier SK; Millet S; Villanueva MP; Berenshteyn F; Song C; Joyner AL. 2005. Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron 45(1):27-40. [PubMed: 15629700] [MGI Ref ID J:95823]
Sillitoe RV; Stephen D; Lao Z; Joyner AL. 2008. Engrailed homeobox genes determine the organization of Purkinje cell sagittal stripe gene expression in the adult cerebellum. J Neurosci 28(47):12150-62. [PubMed: 19020009] [MGI Ref ID J:142371]
Sillitoe RV; Vogel MW; Joyner AL. 2010. Engrailed homeobox genes regulate establishment of the cerebellar afferent circuit map. J Neurosci 30(30):10015-24. [PubMed: 20668186] [MGI Ref ID J:162855]
Srinivas S; Watanabe T; Lin CS; William CM; Tanabe Y; Jessell TM; Costantini F. 2001. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1(1):4. [PubMed: 11299042] [MGI Ref ID J:80963]
Sudarov A; Turnbull RK; Kim EJ; Lebel-Potter M; Guillemot F; Joyner AL. 2011. Ascl1 genetics reveals insights into cerebellum local circuit assembly. J Neurosci 31(30):11055-69. [PubMed: 21795554] [MGI Ref ID J:174604]
Sunmonu NA; Chen L; Li JY. 2009. Misexpression of Gbx2 throughout the mesencephalon by a conditional gain-of-function transgene leads to deletion of the midbrain and cerebellum in mice. Genesis 47(10):667-673. [PubMed: 19603509] [MGI Ref ID J:153564]
Trokovic R; Jukkola T; Saarimaki J; Peltopuro P; Naserke T; Weisenhorn DM; Trokovic N; Wurst W; Partanen J. 2005. Fgfr1-dependent boundary cells between developing mid- and hindbrain. Dev Biol 278(2):428-39. [PubMed: 15680361] [MGI Ref ID J:104600]
Trokovic R; Trokovic N; Hernesniemi S; Pirvola U; Vogt Weisenhorn DM; Rossant J; McMahon AP; Wurst W; Partanen J. 2003. FGFR1 is independently required in both developing mid- and hindbrain for sustained response to isthmic signals. EMBO J 22(8):1811-23. [PubMed: 12682014] [MGI Ref ID J:83004]
Wakabayashi J; Zhang Z; Wakabayashi N; Tamura Y; Fukaya M; Kensler TW; Iijima M; Sesaki H. 2009. The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice. J Cell Biol 186(6):805-16. [PubMed: 19752021] [MGI Ref ID J:153835]
Wong YL; Behringer RR; Kwan KM. 2012. Smad1/Smad5 signaling in limb ectoderm functions redundantly and is required for interdigital programmed cell death. Dev Biol 363(1):247-57. [PubMed: 22240098] [MGI Ref ID J:182768]
Yu T; Yaguchi Y; Echevarria D; Martinez S; Basson MA. 2011. Sprouty genes prevent excessive FGF signalling in multiple cell types throughout development of the cerebellum. Development 138(14):2957-68. [PubMed: 21693512] [MGI Ref ID J:173529]
Zervas M; Millet S; Ahn S; Joyner AL. 2004. Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1. Neuron 43(3):345-57. [PubMed: 15294143] [MGI Ref ID J:152670]
Zhao Y; Kwan KM; Mailloux CM; Lee WK; Grinberg A; Wurst W; Behringer RR; Westphal H. 2007. LIM-homeodomain proteins Lhx1 and Lhx5, and their cofactor Ldb1, control Purkinje cell differentiation in the developing cerebellum. Proc Natl Acad Sci U S A 104(32):13182-6. [PubMed: 17664423] [MGI Ref ID J:123471]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype siblings. Homozygotes die at birth.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.