Strain Name:

STOCK En1tm7(cre/ESR1)Alj/J

Stock Number:

007917

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Availability:

Cryopreserved - Ready for recovery

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En1-CreERT1 mutant mice harbor the Cre-ERT1 fusion gene under control of the endogenous engrailed 1 locus. When En1-CreERT1 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in En1-expressing tissues (including the mesencephalon and rhombomere 1, as well as the developing cerebellum, dermis, and limbs).

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Alexandra L Joyner,   Memorial Sloan-Kettering Cancer Center

Description
While mice heterozygous for this En1-CreERT1 mutation are viable and fertile, homozygotes die at birth. Because the Cre-ERT1 fusion gene is inserted between the transcriptional start site and the first exon of the engrailed 1 (En1) gene, tamoxifen-inducible cre activity is controlled by the endogenous En1 regulatory elements. The Cre-ERT1 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT1 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these En1-CreERT1 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in En1-expressing tissues (including the mesencephalon and rhombomere 1, as well as the developing cerebellum, dermis, and limbs).

Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007916, Stock No. 007918, Stock No. 007924, and Stock No. 007925).

Development
A targeting construct was designed with a CreERT1 fusion gene (Cre-ERT1; Cre recombinase fused to a G400V/L539A/L540A triple mutation of the human estrogen receptor ligand binding domain) followed by a reverse-oriented loxP-flanked neo cassette. This construct was inserted between the transcriptional start site and first exon of the targeted gene (replacing the translational start site) via electroporation into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred to Black Swiss females. The resulting En1-CreERT1-neo heterozygotes were then bred to cre expressing mice (on a Swiss Webster genetic background) to remove the neo cassette, generating En1-CreERT1 offspring (with a single loxP site remaining after the Cre-ERT1 fusion gene). These En1-CreERT1 mutant mice were bred to Swiss Webster mice for many generations prior to arrival at The Jackson Laboratory. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, En1-CreERT1 mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of En1
003343   B6.129S2-En1tm1Alj/J
002656   STOCK En1tm1Alj/J
007916   STOCK En1tm2(cre)Wrst/J
007912   STOCK En1tm2Alj/J
007918   STOCK En1tm8.1Alj/J
View Strains carrying other alleles of En1     (5 strains)

View Strains carrying other alleles of cre/Esr1     (15 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Perinatal Lethality
      Homozygous

Neurobiology Research
Cre-lox System
      Cre Recombinase expression in neural tissue

Research Tools
Cre-lox System
      Cre Recombinase Expression
      Cre Recombinase Expression: Inducible
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System
      Tissue/Cell Markers
      Tissue/Cell Markers: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol En1tm7(cre/ESR1)Alj
Allele Name targeted mutation 7, Alexandra L Joyner
Allele Type Targeted (Inducible, cre- or Flp-expressing)
Common Name(s) En1-CreERT1; En1-CreERT1; En1tm7(cre)Alj; Engrailed1-Cre;
Mutation Made ByDr. Alexandra Joyner,   Memorial Sloan-Kettering Cancer Center
Strain of Origin129S6/SvEvTac
ES Cell Line NameW4
ES Cell Line Strain129S6/SvEvTac
Site of Expressionmesencephalon and rhombomere 1, as well as the developing cerebellum, dermis, and limbs (after induction)
Expressed Gene cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene,
Gene Symbol and Name En1, engrailed 1
Chromosome 1
Gene Common Name(s) En-1; Mo-en.1; engrailed-1;
Driver Note En1
Inducible Note induced by tamoxifen
Molecular Note A tamoxifen dependent form of cre recombinase (CreER) and a loxP-flanked neo in reverse orientation were inserted into the locus. The neo was removed in vivo via Cre-mediated recombination. [MGI Ref ID J:95823]
 

Genotyping

Genotyping Information

Genotyping Protocols

En1tm7(cre/ESR1)Alj-Alternate 2, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sgaier SK; Millet S; Villanueva MP; Berenshteyn F; Song C; Joyner AL. 2005. Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron 45(1):27-40. [PubMed: 15629700]  [MGI Ref ID J:95823]

Additional References

En1tm7(cre/ESR1)Alj related

Deckelbaum RA; Holmes G; Zhao Z; Tong C; Basilico C; Loomis CA. 2012. Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1. Development 139(7):1346-58. [PubMed: 22395741]  [MGI Ref ID J:184618]

Guo Q; Li K; Sunmonu NA; Li JY. 2010. Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Dev Biol 338(2):183-92. [PubMed: 19968985]  [MGI Ref ID J:156717]

Kala K; Jukkola T; Pata I; Partanen J. 2008. Analysis of the midbrain-hindbrain boundary cell fate using a boundary cell-specific Cre-mouse strain. Genesis 46(1):29-36. [PubMed: 18196597]  [MGI Ref ID J:135242]

Ohtola J; Myers J; Akhtar-Zaidi B; Zuzindlak D; Sandesara P; Yeh K; Mackem S; Atit R. 2008. {beta}-Catenin has sequential roles in the survival and specification of ventral dermis. Development 135(13):2321-9. [PubMed: 18539925]  [MGI Ref ID J:137352]

Sgaier SK; Lao Z; Villanueva MP; Berenshteyn F; Stephen D; Turnbull RK; Joyner AL. 2007. Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins. Development 134(12):2325-35. [PubMed: 17537797]  [MGI Ref ID J:134516]

Sillitoe RV; Stephen D; Lao Z; Joyner AL. 2008. Engrailed homeobox genes determine the organization of Purkinje cell sagittal stripe gene expression in the adult cerebellum. J Neurosci 28(47):12150-62. [PubMed: 19020009]  [MGI Ref ID J:142371]

Sillitoe RV; Vogel MW; Joyner AL. 2010. Engrailed homeobox genes regulate establishment of the cerebellar afferent circuit map. J Neurosci 30(30):10015-24. [PubMed: 20668186]  [MGI Ref ID J:162855]

Tran TH; Jarrell A; Zentner GE; Welsh A; Brownell I; Scacheri PC; Atit R. 2010. Role of canonical Wnt signaling/ss-catenin via Dermo1 in cranial dermal cell development. Development 137(23):3973-84. [PubMed: 20980404]  [MGI Ref ID J:166907]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype siblings. The donating investigator reports that homozygotes die at birth. Of note, to ensure the mutant phenotype of these En1-mutant mice, avoid crossing these mice onto the C57BL/6 genetic background (as the phenotype of En1-null mice is rescued on the C57BL/6 genetic background).

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2625.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3412.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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