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| Mice homozygous for this Gli2lzki allele harbor a β-galactosidase "knock-in" (lzki) mutation that also abolishes endogenous GLI-Kruppel family member GLI2 (Gli2) gene function, and may be useful for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Heterozygote (Female x Male) 10-MAR-09 Species laboratory mouse Generation F?+F11 (24-AUG-11)
Generation DefinitionsDonating Investigator Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center Description
Mice homozygous for this Gli2lzki allele harbor a β-galactosidase "knock-in" (lzki) allele that also abolishes endogenous gene function. As such, homozygous mice die at birth with many defects including absence of Sonic Hedgehog-expressing floor plate cells, reduction of Nkx2.2-expressing V3 interneurons in the spinal cord, and defects in midbrain, cerebellum and lung development. Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern identical to the wild-type gene. Heterozygotes are viable and fertile. These Gli2lzki mice may be useful for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs.Development
A targeting vector was designed with a nuclear localized β-galactosidase cDNA (with 1.7 kb of 3' UTR and three tandem SV40 poly A sequence repeats downstream) and followed by a reverse-oriented loxP-flanked neo cassette. This construct was used to replace a portion of the first coding exon (exon 2) of the targeted gene (including the ATG start codon) via electroporation into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss Webster outbred mice. The resulting Gli2nlzki heterozygotes were then bred to cre expressing mice (on a Swiss Webster genetic background) to remove the neo cassette, generating Gli2lzki offspring (with a single loxP site remaining after the SV40 polyA). These Gli2lzki mutant mice were bred to Swiss Webster mice for many generations prior to arrival at The Jackson Laboratory. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, Gli2lzki mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
007926 STOCK Gli2tm6Alj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Holoprosencephaly 9; HPE9 (GLI2)
assigned by genotype
Gli2tm2.1Alj/Gli2tm2.1Alj
either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss)
- nervous system phenotype
- abnormal spinal cord morphology
- abnormal spinal cord interneuron morphology
- decrease in the number of spinal cord V3 interneurons that are normally adjacent to the floor plate at E10.5 (MGI Ref ID J:73074)
- absent floor plate
- at E10.5 cells that express Shh and Foxa2 are absent (MGI Ref ID J:73074)
- embryogenesis phenotype
- abnormal notochord morphology
- persistence of notochord tissue
- the notochord fails to regress (MGI Ref ID J:73074)
- absent floor plate
- at E10.5 cells that express Shh and Foxa2 are absent (MGI Ref ID J:73074)
- respiratory system phenotype
- absent right lung accessory lobe
- the accessory lobe is absent (MGI Ref ID J:73074)
- small lung (MGI Ref ID J:73074)
Gli2tm2.1Alj/Gli2tm2.1Alj
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster
- mortality/aging
- perinatal lethality (MGI Ref ID J:112460)
- reproductive system phenotype
- *normal* reproductive system phenotype
- embryos show normal mammary gland development at E13 (MGI Ref ID J:112460)
Gli2tm2.1Alj/Gli2tm2.1Alj
involves: 129S6/SvEvTac * C57BL/6 * Swiss Webster
- embryogenesis phenotype
- abnormal floor plate morphology
- the Nkx2-2 expressing domains are absent and the motor neurons appear fused together (MGI Ref ID J:169530)
- nervous system phenotype
- abnormal floor plate morphology
- the Nkx2-2 expressing domains are absent and the motor neurons appear fused together (MGI Ref ID J:169530)
- abnormal motor neuron morphology
- in the floor plate the motor neurons appear fused together (MGI Ref ID J:169530)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
brain
lung
multiple
Neurodevelopmental Defects
Perinatal Lethality
Homozygous
Neurobiology Research
lacZ expression in neural tissue
Neurodevelopmental Defects
Research Tools
lacZ Expression
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: multiple
Tissue/Cell Markers: neurons
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gli2tm2.1Alj | ||
|---|---|---|---|
| Allele Name | targeted mutation 2.1, Alexandra L Joyner | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Gli2lacZ; Gli2lz; Gli2lzki; | ||
| Mutation Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | LacZ expression is observed in a pattern identical to the wild-type targeted gene. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A lacZ gene and a loxP-flanked neomycin selection cassette replaced 56 bp of exon 2, including the initiation codon. The neomycin selection cassette was removed by Cre mediated recombination in mice by crossing Gli2tm2Alj mice with NLS-cre expressing mice, and selecting for offspring that no longer had the neomycin cassette. [MGI Ref ID J:73074] | ||
| Gene Symbol and Name | Gli2, GLI-Kruppel family member GLI2 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | AW546128; HPE9; THP1; THP2; expressed sequence AW546128; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gli2tm2.1Alj,Separated MCA
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Bai CB; Joyner AL. 2001. Gli1 can rescue the in vivo function of Gli2. Development 128(24):5161-72. [PubMed: 11748151] [MGI Ref ID J:73074]
Additional References
Gli2tm2.1Alj relatedBarsoum I; Yao HH. 2011. Redundant and differential roles of transcription factors gli1 and gli2 in the development of mouse fetal Leydig cells. Biol Reprod 84(5):894-9. [PubMed: 21209421] [MGI Ref ID J:173724]
Cho A; Ko HW; Eggenschwiler JT. 2008. FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism. Dev Biol 321(1):27-39. [PubMed: 18590716] [MGI Ref ID J:138713]
Christopher KJ; Wang B; Kong Y; Weatherbee SD. 2012. Forward genetics uncovers Transmembrane protein 107 as a novel factor required for ciliogenesis and Sonic hedgehog signaling. Dev Biol 368(2):382-92. [PubMed: 22698544] [MGI Ref ID J:186552]
Corrales JD; Rocco GL; Blaess S; Guo Q; Joyner AL. 2004. Spatial pattern of sonic hedgehog signaling through Gli genes during cerebellum development. Development 131(22):5581-90. [PubMed: 15496441] [MGI Ref ID J:94461]
Fabian SL; Penchev RR; St-Jacques B; Rao AN; Sipila P; West KA; McMahon AP; Humphreys BD. 2012. Hedgehog-Gli Pathway Activation during Kidney Fibrosis. Am J Pathol 180(4):1441-53. [PubMed: 22342522] [MGI Ref ID J:182155]
Garcia AD; Petrova R; Eng L; Joyner AL. 2010. Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain. J Neurosci 30(41):13597-608. [PubMed: 20943901] [MGI Ref ID J:165495]
Hatsell SJ; Cowin P. 2006. Gli3-mediated repression of Hedgehog targets is required for normal mammary development. Development 133(18):3661-70. [PubMed: 16914490] [MGI Ref ID J:112460]
Li J; Wang C; Pan Y; Bai Z; Wang B. 2011. Increased proteolytic processing of full-length Gli2 transcription factor reduces the hedgehog pathway activity in vivo. Dev Dyn 240(4):766-74. [PubMed: 21337666] [MGI Ref ID J:169530]
Liu J; Heydeck W; Zeng H; Liu A. 2012. Dual function of suppressor of fused in Hh pathway activation and mouse spinal cord patterning. Dev Biol 362(2):141-53. [PubMed: 22182519] [MGI Ref ID J:180775]
Mukhopadhyay S; Wen X; Ratti N; Loktev A; Rangell L; Scales SJ; Jackson PK. 2013. The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling. Cell 152(1-2):210-23. [PubMed: 23332756] [MGI Ref ID J:193395]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype siblings. Homozygotes die at birth. Mating System +/+ sibling x Heterozygote (Female x Male) 10-MAR-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Gli2tm2.1Alj
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Gli2tm2.1Alj x Wild-type for Gli2tm2.1Alj $296.00 Wild-type for Gli2tm2.1Alj x Heterozygous for Gli2tm2.1Alj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Gli2tm2.1Alj
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Gli2tm2.1Alj x Wild-type for Gli2tm2.1Alj $384.80 Wild-type for Gli2tm2.1Alj x Heterozygous for Gli2tm2.1Alj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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