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| The 5 strains that comprise this NOD congenic set (Stock No.'s 007930, 007931, 007932, 007933, 007934) contain the Idd 3 region from non-NOD strains. All of these strains are viable and fertile. Stocks containing the Idd 3 region from SWR/J, A/J, or CAST/EiJ are diabetes susceptible, while the congenic stocks containing the Idd 3 region derived from C57BL/6 or CZECHI/EiJ are diabetes resistant and produce more IL2 mRNA than the diabetes susceptible strains. These strains have been used to positionally clone Idd3 on Chr. 3 and continue to be valued to study the biological effects of variation of genes within the Idd3 region and for studying long-range chromosome remodeling. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Background Strain NOD/MrkTac Donor Strain CAST/EiJ H2 Haplotype g7 Generation N6F11 (06-NOV-07) Donating Investigator Linda Wicker, Appearance
Albino
Related Genotype: A/A Tyrc/TyrcDescription
This NOD congenic strain, commonly referred to as NOD.CAST-Idd3, is carrying Chr 3 alleles, SNPs rs3022948 (7.43Mb) through rs13477164, (66.638Mb) derived from strain CAST/EiJ including the insulin dependent diabetes susceptibility 3 loci (Idd3). Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac. Thymocytes and splenocytes of this T1D susceptible stock produce 2 fold less Il2 mRNA than T1D protected strains (Stock No's. 007933 and 007934). This congenic strain, one of a set of 5 strains (Stock No's. 007930, 007931, 007933, 007934), has been useful to positionally clone Idd3 and to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 gene and continue to be valued for studying long-range chromosome remodeling.Development
A CAST/EiJ (Stock No.000928) congenic segment is 29.8Mb in length and is defined within SNPs rs3022948 (7.43Mb) and rs3140619 (37.3Mb) that includes the Idd3 locus on Chr 3. Idd3 contains major candidate gene IL2 as well as IL21, Tenr, Cetn4 and Fgf2. The CAST/EiJ congenic segment was introgressed onto the NOD/MrkTac background. This strain was produced by mating mice from NOD/MrkTac to CAST/EiJ followed by 5 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the CAST/EiJ derived segment denoted by makers D3Mit329 through D3Mit274 were intercrossed to make homozygous. 5K SNP Chip Data, based on NCBI, build 36, identifies the congenic interval between SNPs rs6168642 (18.46Mb) and rs13477164 (66.15Mb) in addition a contaminating 10.9Mb CAST/EiJ region from Chr 9 defined between SNPs rs13480413 (109.73Mb) and rs6244221 (120.69MB) was identified. In 2007, the T1DR received this strain at generation N6F11.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Idd3
003067 B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J 003059 B6.NOD-(D3Mit132-Tshb)/J 007931 NOD.A-Idd3A/J/MrkJ 003862 NOD.B6(PL)-(D3Mit167-D3Mit94)/Lt 003853 NOD.B6(PL)-(D3Mit93-D3Mit124)/Lt 007934 NOD.B6(PL)-Idd3C57BL/6/MrkTacJ 007933 NOD.CZI-Idd3CZECHI/EiJ/MrkTacJ 007930 NOD.SWR-Idd3SWR/J/MrkJ View Strains carrying other alleles of Idd3 (8 strains)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
assigned by genotype
Idd3CAST/EiJ/Idd3CAST/EiJ
NOD.CAST-Idd3CAST/EiJ
- immune system phenotype
- increased susceptibility to autoimmune diabetes (MGI Ref ID J:120349)
- susceptibility to type 1 diabetes
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Idd3CAST/EiJ | ||
|---|---|---|---|
| Allele Name | CAST/EiJ | ||
| Allele Type | QTL | ||
| Strain of Origin | CAST/EiJ | ||
| Gene Symbol and Name | Idd3, insulin dependent diabetes susceptibility 3 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | Idd-3; | ||
| Molecular Note | This allele confers susceptibility to type 1 diabetes compared to C57BL/6J and CZECHI/EiJ. Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac. [MGI Ref ID J:120349] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778] [MGI Ref ID J:120349]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $54.00 Female or Male Homozygous for Idd3CAST/EiJ *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $108.00 Homozygous for Idd3CAST/EiJ x Homozygous for Idd3CAST/EiJ
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $70.20 Female or Male Homozygous for Idd3CAST/EiJ *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $140.40 Homozygous for Idd3CAST/EiJ x Homozygous for Idd3CAST/EiJ
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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