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Strain Name:

NOD.CAST-Idd3CAST/EiJ/MrkJ

Stock Number:

007932

Availability:

Repository- Live

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General Terms and Conditions

Strain Common Names      NOD.CAST-Idd3;
Genes & Alleles   Idd3;   Idd3CAST/EiJ;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain NOD/MrkTac
Donor Strain CAST/EiJ
Donating Investigator Linda Wicker,  
H2 Haplotypeg7
GenerationN6F11 (06-NOV-07)

Appearance
Albino
Related Genotype: A/A Tyrc/Tyrc

Strain Description
This NOD congenic strain, commonly referred to as NOD.CAST-Idd3, is carrying Chr 3 alleles, SNPs rs3022948 (7.43Mb) through rs13477164, (66.638Mb) derived from strain CAST/EiJ including the insulin dependent diabetes susceptibility 3 loci (Idd3). Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac. Thymocytes and splenocytes of this T1D susceptible stock produce 2 fold less Il2 mRNA than T1D protected strains (Stock No's. 007933 and 007934). This congenic strain, one of a set of 5 strains (Stock No's. 007930, 007931, 007933, 007934), has been useful to positionally clone Idd3 and to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 gene and continue to be valued for studying long-range chromosome remodeling.

Strain Development
A CAST/EiJ (Stock No.000928) congenic segment is 29.8Mb in length and is defined within SNPs rs3022948 (7.43Mb) and rs3140619 (37.3Mb) that includes the Idd3 locus on Chr 3. Idd3 contains major candidate gene IL2 as well as IL21, Tenr, Cetn4 and Fgf2. The CAST/EiJ congenic segment was introgressed onto the NOD/MrkTac background. This strain was produced by mating mice from NOD/MrkTac to CAST/EiJ followed by 5 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the CAST/EiJ derived segment denoted by makers D3Mit329 through D3Mit274 were intercrossed to make homozygous. 5K SNP Chip Data, based on NCBI, build 36, identifies the congenic interval between SNPs rs6168642 (18.46Mb) and rs13477164 (66.15Mb) in addition a contaminating 10.9Mb CAST/EiJ region from Chr 9 defined between SNPs rs13480413 (109.73Mb) and rs6244221 (120.69MB) was identified. In 2007, the T1DR received this strain at generation N6F11.

Mammalian Phenotype Terms assigned by genotype

Idd3CAST/EiJ/Idd3CAST/EiJ

        NOD.CAST-Idd3CAST/EiJ
  • immune system phenotype
  • increased susceptibility to autoimmune diabetes (MGI Ref ID J:120349)
    • susceptibility to type 1 diabetes

Gene & Allele Details

Allele Symbol Idd3CAST/EiJ
Allele Name CAST/EiJ
Strain of OriginCAST/EiJ
Gene Symbol and Name Idd3, insulin dependent diabetes susceptibility 3
Chromosome 3
Gene Common Name(s) Idd-3;
Molecular Note This allele confers susceptibility to type 1 diabetes compared to C57BL/6J and CZECHI/EiJ. Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac. [MGI Ref ID J:120349]

Control Information

  Control
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of Idd3     (8 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)

References

Selected Reference(s)

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Price and Supply Information

Strain Name: NOD.CAST-Idd3CAST/EiJ/MrkJ
Stock Number: 007932

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Type 1 Diabetes Repository collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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