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Strain Name:

NOD.CZI-Idd3CZECHI/EiJ/MrkTacJ

Stock Number:

007933

Availability:

Repository- Live

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General Terms and Conditions

Strain Common Names      NOD.CZECH-Idd3;
Genes & Alleles   Idd3;   Idd3CZECHI/EiJ;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain NOD/MrkTac
Donor Strain CZECHI/EiJ
Donating Investigator Linda Wicker,  
H2 Haplotypeg7
GenerationN6F25+F1 (28-FEB-08)

Appearance
Albino
Related Genotype: A/A Tyrc/Tyrc

Strain Description
This NOD congenic strain, commonly referred to as NOD.CZECH-Idd3, is carrying Chr 3 alleles, rs13477023 (20.01Mb) through rs13477134 (58.91Mb), derived from strain CZECH/EiJ including the insulin dependent diabetes susceptibility 3 loci (Idd3). Il2 exon 1 sequence and glycosylation pattern is similar to NOD/MrkTac. Surprisingly, the diabetes frequency of this strain of mice is identical to that of NOD.B6-Idd3 mice. Activated and non-activated thymocytes and splenocytes of this T1D protected stock produce 2 fold more Il2 mRNA than T1D susceptible strains (Stock No's. 007930, 007931, and 007932). However, the NOD.CZECH-Idd3 congenic produces more Il2 mRNA than the NOD.B6-Idd3 congenic. This congenic strain, one of a set of 5 strains (Stock No's 007930, 007931, 007932, and 007934), has been useful to positionally clone Idd3, to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 gene and continue to be valued for studying long-range chromosome remodeling.

Strain Development
A CZECHI/EiJ (Stock No.002799) congenic segment is 32.9Mb in length and is defined within SNPs rs13477023 (20.01Mb) and rs13477134 (58.91Mb) that includes the Idd3 locus on Chr 3. Idd3 contains major candidate gene IL2 as well as IL21, Tenr, Cetn4 and Fgf2. CZECHI/EiJ mice are wild derived and have a greater diversity of sequence variants than inbred mice. The CZECHI/EiJ congenic segment was introgressed onto the NOD/MrkTac background. This strain was produced by mating mice from NOD/MrkTac to CZECHI/EiJ followed by 5 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the CZECHI/EiJ derived segment denoted by makers D3Mit329 through D3Mit274 were intercrossed to make homozygous. 5K SNP Chip Data, based on NCBI, build 36, identifies the congenic interval between SNPs rs13477023 (20.01Mb) and rs13477134 (58.91Mb) in addition a contaminating 3.45Mb CZECHI/EiJ region on Chr 1 defined between SNPs rs13476237 (173.54Mb) and rs13478254 (176.99Mb) and SNP rs13476254 (176.99Mb) on Chr 13 was identified. In 2007, the T1DR received this strain at generation N6F25. Genetic quality control performed at The Jackson Laboratory did not identify the disclosed contamination identified by SNP's on Chr.'s 1 and 13 with the Mit markers that were used. However marker D18Mit208 has been identified as CZECH/EiJ in Origin.

Mammalian Phenotype Terms assigned by genotype

Idd3CZECHI/EiJ/Idd3CZECHI/EiJ

        NOD.CZECH-Idd3CZECHI/EiJ
  • immune system phenotype
  • decreased susceptibility to autoimmune diabetes (MGI Ref ID J:120349)
    • resistance to type 1 diabetes

Gene & Allele Details

Allele Symbol Idd3CZECHI/EiJ
Allele Name CZECHI/EiJ
Strain of OriginCZECHI/EiJ
Gene Symbol and Name Idd3, insulin dependent diabetes susceptibility 3
Chromosome 3
Gene Common Name(s) Idd-3;
Molecular Note This allele confers resistance to type 1 diabetes compared to A/J, CAST/EiJ, NOD, and SWR/J. The diabetes frequency of this strain of mice is identical to that of NOD.B6-Idd3 mice. [MGI Ref ID J:120349]

Control Information

  Control
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of Idd3     (8 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           FGB29

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)

References

Selected Reference(s)

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Price and Supply Information

Strain Name: NOD.CZI-Idd3CZECHI/EiJ/MrkTacJ
Stock Number: 007933

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Type 1 Diabetes Repository collection.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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