Strain Name:

NOD.B6(PL)-Idd3C57BL/6/MrkTacJ

Stock Number:

007934

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Common Names: NOD.B6-Idd3;    
The five strains that comprise this NOD congenic set (Stock No.'s 007930, 007931, 007932, 007933, 007934) contain the Idd3 region from non-NOD strains. All of these strains are viable and fertile. Stocks containing the Idd3 region from SWR/J, A/J, or CAST/EiJ are diabetes susceptible, while the congenic stocks containing the Idd3 region derived from C57BL/6 or CZECHI/EiJ are diabetes resistant and produce more IL2 mRNA than the diabetes susceptible strains. These strains have been used to positionally clone Idd3 on Chromosome 3 and continue to be valued to study the biological effects of variation of genes within the Idd3 region and for studying long-range chromosome remodeling.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain NOD/MrkTac
Donor Strain B6.PL-Thy1a/CyJ
H2 Haplotypeg7
GenerationN13F25+F2p
Generation Definitions
 
Donating Investigator Linda Wicker,   University of Cambridge, UK

Appearance
Albino
Related Genotype: A/A Tyrc/Tyrc

Description
This NOD congenic strain, commonly referred to as NOD.B6-Idd3, commonly referred to as NOD.B6-Idd3R450, is carrying Chromosome 3 alleles, rs3022959 through rs3140619, derived from strain C57BL/6 including the insulin dependent diabetes susceptibility 3 loci (Idd3). This NOD.B6 congenic strain is diabetes resistant, with approximately 10% of the females becoming diabetic by 250 days of age. Activated and non-activated thymocytes and splenocytes of this T1D protected stock produce more Il2 mRNA than T1D susceptible strains (Stock No's. 007930, 007931, and 007932). This congenic strain, one of a set of 5 strains (Stock No's. 007930, 007931, 007932, and 007933), has been useful to positionally clone Idd3, to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 gene and continues to be valued for studying long-range chromosome remodeling.

Development
C57BL/6 (Type 1 diabetes resistant)-derived portions of the Idd3 locus, located on Chromosome 3, were introgressed onto the genetic background of NOD/MrkTac (Type 1 diabetes susceptible). Idd3 contains major candidate gene IL-2 as well as IL21, Tenr, Cetn4, and Fgf2. This strain was produced by mating mice from NOD/MrkTac to B6.Pl-Thy1a/Cy followed by 12 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the C57BL/6 derived chromosomal segment denoted by markers D3Nds55 (34.9Mb) through D3Nds76 (37.7Mb) were intercrossed to make homozygous. 5kSNP Chip Data based on NCBI, build 36, identifies the congenic interval between SNP's rs3022959 through rs3140619 with no other contamination on other chromosomes. This strain carries Thy1b of NOD origin. In 2007, the T1DR received this strain at generation N13F25.

Control Information

  Control
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Idd3C57BL/6     (4 strains)

View Strains carrying other alleles of Idd3     (7 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Idd3C57BL/6/Idd3C57BL/6

        NOD.B6-Idd3C57BL/6
  • homeostasis/metabolism phenotype
  • abnormal glucose homeostasis
    • resistance to insulin-dependent diabetes   (MGI Ref ID J:108210)
  • immune system phenotype
  • decreased susceptibility to autoimmune diabetes
    • resistance to type 1 diabetes   (MGI Ref ID J:120349)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Idd3C57BL/6/Idd3C57BL/6

        involves: C57BL/6 * NOD
  • homeostasis/metabolism phenotype
  • abnormal glucose homeostasis
    • resistance to insulin dependent diabetes   (MGI Ref ID J:52572)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
      resistant
Type 1 Diabetes (IDDM) Analysis Strains
      NOD/ShiLtJ Non-MHC Congenics

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      Type 1 Diabetes

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Idd3C57BL/6
Allele Name C57BL/6
Allele Type QTL
Strain of OriginC57BL/6
Gene Symbol and Name Idd3, insulin dependent diabetes susceptibility 3
Chromosome 3
Gene Common Name(s) Idd-3;
General Note Idd3 and Idd5 appear to interact additively. C57BL/6-derived alleles at Idd3 in combination with C57BL/10-derived alleles at Idd5 partially reverses autoimmune exocrinopathy markers on an NOD genetic background.

Idd10 and Idd3 interact to promote disease resistance. Homozygosity for C57BL/6-derived alleles at both Idd10 and Idd3 confers significantly increased resistance to type 1 diabetes.

Il2 is a proposed candidate gene for Idd3. The electrophoretic mobility pattern of IL-2 correlates with type 1 diabetes incidence. Resistant strain C57BL/6J displays a heterogenous IL-2 mobility pattern with major protein species ranging from 17-19 kDa while susceptible strains 129 and NOD display a homogenous IL-2 mobility pattern with one major proteinspecies at approximately 21-22 kDa.

Molecular Note This allele confers resistance to insulin dependent diabetes compared to NOD. [MGI Ref ID J:52572]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Additional References

Idd3C57BL/6 related

Brayer J; Lowry J; Cha S; Robinson CP; Yamachika S; Peck AB; Humphreys-Beher MG. 2000. Alleles from chromosomes 1 and 3 of NOD mice combine to influence Sjogren's syndrome-like autoimmune exocrinopathy. J Rheumatol 27(8):1896-904. [PubMed: 10955330]  [MGI Ref ID J:71276]

Encinas JA; Wicker LS; Peterson LB; Mukasa A; Teuscher C; Sobel R; Weiner HL; Seidman CE; Seidman JG; Kuchroo VK. 1999. QTL influencing autoimmune diabetes and encephalomyelitis map to a 0.15-cM region containing Il2. Nat Genet 21(2):158-60. [PubMed: 9988264]  [MGI Ref ID J:52572]

Hamilton-Williams EE; Cheung J; Rainbow DB; Hunter KM; Wicker LS; Sherman LA. 2012. Cellular mechanisms of restored beta-cell tolerance mediated by protective alleles of Idd3 and Idd5. Diabetes 61(1):166-74. [PubMed: 22106155]  [MGI Ref ID J:191380]

Jiang W; Anderson MS; Bronson R; Mathis D; Benoist C. 2005. Modifier loci condition autoimmunity provoked by Aire deficiency. J Exp Med 202(6):805-15. [PubMed: 16172259]  [MGI Ref ID J:107432]

Kornete M; Sgouroudis E; Piccirillo CA. 2012. ICOS-dependent homeostasis and function of Foxp3+ regulatory T cells in islets of nonobese diabetic mice. J Immunol 188(3):1064-74. [PubMed: 22227569]  [MGI Ref ID J:181217]

Lin X; Hamilton-Williams EE; Rainbow DB; Hunter KM; Dai YD; Cheung J; Peterson LB; Wicker LS; Sherman LA. 2013. Genetic Interactions among Idd3, Idd5.1, Idd5.2, and Idd5.3 Protective Loci in the Nonobese Diabetic Mouse Model of Type 1 Diabetes. J Immunol 190(7):3109-20. [PubMed: 23427248]  [MGI Ref ID J:194526]

Lundholm M; Mayans S; Motta V; Lofgren-Burstrom A; Danska J; Holmberg D. 2010. Variation in the Cd3zeta (Cd247) gene correlates with altered T cell activation and is associated with autoimmune diabetes. J Immunol 184(10):5537-44. [PubMed: 20400699]  [MGI Ref ID J:160996]

Lundholm M; Motta V; Lofgren-Burstrom A; Duarte N; Bergman ML; Mayans S; Holmberg D. 2006. Defective induction of CTLA-4 in the NOD mouse is controlled by the NOD allele of Idd3/IL-2 and a novel locus (Ctex) telomeric on chromosome 1. Diabetes 55(2):538-44. [PubMed: 16443792]  [MGI Ref ID J:106844]

Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418]  [MGI Ref ID J:85823]

McGuire HM; Vogelzang A; Hill N; Flodstrom-Tullberg M; Sprent J; King C. 2009. Loss of parity between IL-2 and IL-21 in the NOD Idd3 locus. Proc Natl Acad Sci U S A 106(46):19438-43. [PubMed: 19880748]  [MGI Ref ID J:154769]

Moritoki Y; Tsuda M; Tsuneyama K; Zhang W; Yoshida K; Lian ZX; Yang GX; Ridgway WM; Wicker LS; Ansari AA; Gershwin ME. 2011. B cells promote hepatic inflammation, biliary cyst formation, and salivary gland inflammation in the NOD.c3c4 model of autoimmune cholangitis. Cell Immunol 268(1):16-23. [PubMed: 21349500]  [MGI Ref ID J:170707]

Podolin PL; Denny P; Lord CJ; Hill NJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 1997. Congenic mapping of the insulin-dependent diabetes (Idd) gene, Idd10, localizes two gene mediating the Idd10 effect and eliminates the candidate Fcgr1. J Immunol 159(4):1835-1843. [PubMed: 9257847]  [MGI Ref ID J:42103]

Podolin PL; Wilusz MB; Cubbon RM; Pajvani U; Lord CJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 2000. Differential glycosylation of interleukin 2, the molecular basis for the NOD Idd3 type 1 diabetes gene? Cytokine 12(5):477-82. [PubMed: 10857762]  [MGI Ref ID J:108210]

Sgouroudis E; Albanese A; Piccirillo CA. 2008. Impact of protective IL-2 allelic variants on CD4+ Foxp3+ regulatory T cell function in situ and resistance to autoimmune diabetes in NOD mice. J Immunol 181(9):6283-92. [PubMed: 18941219]  [MGI Ref ID J:140729]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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