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| The five strains that comprise this NOD congenic set (Stock No.'s 007930, 007931, 007932, 007933, 007934) contain the Idd3 region from non-NOD strains. All of these strains are viable and fertile. Stocks containing the Idd3 region from SWR/J, A/J, or CAST/EiJ are diabetes susceptible, while the congenic stocks containing the Idd3 region derived from C57BL/6 or CZECHI/EiJ are diabetes resistant and produce more IL2 mRNA than the diabetes susceptible strains. These strains have been used to positionally clone Idd3 on Chromosome 3 and continue to be valued to study the biological effects of variation of genes within the Idd3 region and for studying long-range chromosome remodeling. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Background Strain NOD/MrkTac Donor Strain B6.PL-Thy1a/CyJ H2 Haplotype g7 Generation N13F25+F1 (12-AUG-08) Donating Investigator Linda Wicker, Appearance
Albino
Related Genotype: A/A Tyrc/TyrcDescription
This NOD congenic strain, commonly referred to as NOD.B6-Idd3, commonly referred to as NOD.B6-Idd3R450, is carrying Chromosome 3 alleles, rs3022959 through rs3140619, derived from strain C57BL/6 including the insulin dependent diabetes susceptibility 3 loci (Idd3). This NOD.B6 congenic strain is diabetes resistant, with approximately 10% of the females becoming diabetic by 250 days of age. Activated and non-activated thymocytes and splenocytes of this T1D protected stock produce more Il2 mRNA than T1D susceptible strains (Stock No's. 007930, 007931, and 007932). This congenic strain, one of a set of 5 strains (Stock No's. 007930, 007931, 007932, and 007933), has been useful to positionally clone Idd3, to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 gene and continues to be valued for studying long-range chromosome remodeling.Development
C57BL/6 (Type 1 diabetes resistant)-derived portions of the Idd3 locus, located on Chromosome 3, were introgressed onto the genetic background of NOD/MrkTac (Type 1 diabetes susceptible). Idd3 contains major candidate gene IL-2 as well as IL21, Tenr, Cetn4, and Fgf2. This strain was produced by mating mice from NOD/MrkTac to B6.Pl-Thy1a/Cy followed by 12 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the C57BL/6 derived chromosomal segment denoted by markers D3Nds55 (34.9Mb) through D3Nds76 (37.7Mb) were intercrossed to make homozygous. 5kSNP Chip Data based on NCBI, build 36, identifies the congenic interval between SNP's rs3022959 through rs3140619 with no other contamination on other chromosomes. This strain carries Thy1b of NOD origin. In 2007, the T1DR received this strain at generation N13F25.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Idd3C57BL/6 allele
003862 NOD.B6(PL)-(D3Mit167-D3Mit94)/Lt 003853 NOD.B6(PL)-(D3Mit93-D3Mit124)/Lt View Strains carrying Idd3C57BL/6 (2 strains)
003067 B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J 003059 B6.NOD-(D3Mit132-Tshb)/J 007931 NOD.A-Idd3A/J/MrkJ 007932 NOD.CAST-Idd3CAST/EiJ/MrkJ 007933 NOD.CZI-Idd3CZECHI/EiJ/MrkTacJ 007930 NOD.SWR-Idd3SWR/J/MrkJ
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
assigned by genotype
Idd3C57BL/6/Idd3C57BL/6
NOD.B6-Idd3C57BL/6
- homeostasis/metabolism phenotype
- abnormal glucose homeostasis (MGI Ref ID J:108210)
- resistance to insulin-dependent diabetes
- immune system phenotype
- decreased susceptibility to autoimmune diabetes (MGI Ref ID J:120349)
- resistance to type 1 diabetes
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Idd3C57BL/6/Idd3C57BL/6
involves: C57BL/6 * NOD
- homeostasis/metabolism phenotype
- abnormal glucose homeostasis (MGI Ref ID J:52572)
- resistance to insulin dependent diabetes
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Idd3C57BL/6 | ||
|---|---|---|---|
| Allele Name | C57BL/6 | ||
| Allele Type | QTL | ||
| Strain of Origin | C57BL/6 | ||
| Gene Symbol and Name | Idd3, insulin dependent diabetes susceptibility 3 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | Idd-3; | ||
| General Note |
Idd3 and Idd5 appear to interact additively. C57BL/6-derived alleles at Idd3 in combination with C57BL/10-derived alleles at Idd5 partially reverses autoimmune exocrinopathy markers on an NOD genetic background. Idd10 and Idd3 interact to promote disease resistance. Homozygosity for C57BL/6-derived alleles at both Idd10 and Idd3 confers significantly increased resistance to type 1 diabetes. Il2 is a proposed candidate gene for Idd3. The electrophoretic mobility pattern of IL-2 correlates with type 1 diabetes incidence. Resistant strain C57BL/6J displays a heterogenous IL-2 mobility pattern with major protein species ranging from 17-19 kDa while susceptible strains 129 and NOD display a homogenous IL-2 mobility pattern with one major proteinspecies at approximately 21-22 kDa. | ||
| Molecular Note | This allele confers resistance to insulin dependent diabetes compared to NOD. [MGI Ref ID J:52572] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778] [MGI Ref ID J:120349]
Additional References
Idd3C57BL/6 relatedBrayer J; Lowry J; Cha S; Robinson CP; Yamachika S; Peck AB; Humphreys-Beher MG. 2000. Alleles from chromosomes 1 and 3 of NOD mice combine to influence Sjogren's syndrome-like autoimmune exocrinopathy. J Rheumatol 27(8):1896-904. [PubMed: 10955330] [MGI Ref ID J:71276]
Encinas JA; Wicker LS; Peterson LB; Mukasa A; Teuscher C; Sobel R; Weiner HL; Seidman CE; Seidman JG; Kuchroo VK. 1999. QTL influencing autoimmune diabetes and encephalomyelitis map to a 0.15-cM region containing Il2. Nat Genet 21(2):158-60. [PubMed: 9988264] [MGI Ref ID J:52572]
Jiang W; Anderson MS; Bronson R; Mathis D; Benoist C. 2005. Modifier loci condition autoimmunity provoked by Aire deficiency. J Exp Med 202(6):805-15. [PubMed: 16172259] [MGI Ref ID J:107432]
Lundholm M; Motta V; Lofgren-Burstrom A; Duarte N; Bergman ML; Mayans S; Holmberg D. 2006. Defective induction of CTLA-4 in the NOD mouse is controlled by the NOD allele of Idd3/IL-2 and a novel locus (Ctex) telomeric on chromosome 1. Diabetes 55(2):538-44. [PubMed: 16443792] [MGI Ref ID J:106844]
Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418] [MGI Ref ID J:85823]
Podolin PL; Denny P; Lord CJ; Hill NJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 1997. Congenic mapping of the insulin-dependent diabetes (Idd) gene, Idd10, localizes two gene mediating the Idd10 effect and eliminates the candidate Fcgr1. J Immunol 159(4):1835-1843. [PubMed: 9257847] [MGI Ref ID J:42103]
Podolin PL; Wilusz MB; Cubbon RM; Pajvani U; Lord CJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 2000. Differential glycosylation of interleukin 2, the molecular basis for the NOD Idd3 type 1 diabetes gene? Cytokine 12(5):477-82. [PubMed: 10857762] [MGI Ref ID J:108210]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $54.00 Female or Male Homozygous
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $70.20 Female or Male Homozygous
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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