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| Mice that are homozygous for this mutation may be useful in studies of thrombosis and platelet aggregation. Thrombin-treated mutants exhibit reduced pathological thrombosis and impaired platelet aggregation. | |||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N6+ (12-MAY-08) Donating Investigator Greg Lemke, The Salk Institute Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of brain lysate. Homozygotes exhibit decreased sensitivity to induced pathological thrombosis resulting in reduced fatal pulmonary embolism (25% of wild-type). Platelet aggregation and clot retraction after thrombin treatment is impaired. Platelet signaling and secretion is also defective. Activity induced seizures can occur in mutant mice over the age of seven months. This mutant mouse strain may be useful in studies of thrombosis and platelet aggregation.Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 9 and part of exon 10. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting animals were crossed to C57BL/6 mice and then made homozygous. The mice were then backcrossed to C57BL/6 for five generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tyro3tm1Grl/Tyro3tm1Grl
Background Not Specified
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:54681)
- mice were viable and fertile
This mouse can be used to support research in many areas including:
Cardiovascular Research
Vascular Defects (Thrombosis)
Vascular Defects (platelet defect)
Hematological Research
Platelet Defects (Alterations in platelet aggregation)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tyro3tm1Grl | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Greg Lemke | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Tyro3; | ||
| Mutation Made By | Greg Lemke, The Salk Institute | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Tyro3, TYRO3 protein tyrosine kinase 3 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AI323366; BYK; Brt; Dtk; Etk-2; FLJ16467; RSE; Sky; Tif; brain tyrosine kinase; expressed sequence AI323366; | ||
| Molecular Note | A neomycin selection cassette replaced sequences containing exon 9 and part of exon 10. Western blot analysis on brain lysates derived from homozygous mice confirmed that no detectable protein was produced from this allele. [MGI Ref ID J:54681] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tyro3tm1Grl, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Lu Q; Gore M; Zhang Q; Camenisch T; Boast S; Casagranda F; Lai C; Skinner MK; Klein R; Matsushima GK; Earp HS; Goff SP; Lemke G. 1999. Tyro-3 family receptors are essential regulators of mammalian spermatogenesis. Nature 398(6729):723-8. [PubMed: 10227296] [MGI Ref ID J:54681]
Additional References
Tyro3tm1Grl relatedAngelillo-Scherrer A; Burnier L; Flores N; Savi P; DeMol M; Schaeffer P; Herbert JM; Lemke G; Goff SP; Matsushima GK; Earp HS; Vesin C; Hoylaerts MF; Plaisance S; Collen D; Conway EM; Wehrle-Haller B; Carmeliet P. 2005. Role of Gas6 receptors in platelet signaling during thrombus stabilization and implications for antithrombotic therapy. J Clin Invest 115(2):237-46. [PubMed: 15650770] [MGI Ref ID J:95952]
Angelillo-Scherrer A; Burnier L; Lambrechts D; Fish RJ; Tjwa M; Plaisance S; Sugamele R; DeMol M; Martinez-Soria E; Maxwell PH; Lemke G; Goff SP; Matsushima GK; Earp HS; Chanson M; Collen D; Izui S; Schapira M; Conway EM; Carmeliet P. 2008. Role of Gas6 in erythropoiesis and anemia in mice. J Clin Invest 118(2):583-96. [PubMed: 18188450] [MGI Ref ID J:131177]
Lu Q; Lemke G. 2001. Homeostatic regulation of the immune system by receptor tyrosine kinases of the Tyro 3 family. Science 293(5528):306-11. [PubMed: 11452127] [MGI Ref ID J:70420]
Prasad D; Rothlin CV; Burrola P; Burstyn-Cohen T; Lu Q; Garcia de Frutos P; Lemke G. 2006. TAM receptor function in the retinal pigment epithelium. Mol Cell Neurosci 33(1):96-108. [PubMed: 16901715] [MGI Ref ID J:116526]
Radic MZ; Shah K; Zhang W; Lu Q; Lemke G; Hilliard GM. 2006. Heterogeneous nuclear ribonucleoprotein P2 is an autoantibody target in mice deficient for Mer, Axl, and Tyro3 receptor tyrosine kinases. J Immunol 176(1):68-74. [PubMed: 16365397] [MGI Ref ID J:126253]
Rothlin CV; Ghosh S; Zuniga EI; Oldstone MB; Lemke G. 2007. TAM receptors are pleiotropic inhibitors of the innate immune response. Cell 131(6):1124-36. [PubMed: 18083102] [MGI Ref ID J:131447]
Xiong W; Chen Y; Wang H; Wang H; Wu H; Lu Q; Han D. 2008. Gas6 and the Tyro 3 receptor tyrosine kinase subfamily regulate the phagocytic function of Sertoli cells. Reproduction 135(1):77-87. [PubMed: 18159085] [MGI Ref ID J:133455]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Tyro3tm1Grl *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Tyro3tm1Grl x Heterozygous for Tyro3tm1Grl
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Tyro3tm1Grl *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Tyro3tm1Grl x Heterozygous for Tyro3tm1Grl
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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