Strain Name: |
B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J |
|---|---|
Stock Number: |
007940 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | CFP; COX8A; Thy1; Tg(Thy1-CFP/COX8A)C1Lich; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Noncarrier (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N3 (20-DEC-07) Strain Description
These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations including retinal cells and in all motor axons. Coronal brain sections reveal a fluorescence pattern showing somatosensory cortex barrel morphology. Neuronal, mitochondrial and neuromuscular junction morphology appears normal in transgenic mice. Axonal mitochondrial density is similar to wildtype. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of mitochondrial transport.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBA mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published line C, was subsequently established. The mice were backcrossed for at least 5 generations to C57BL/6J.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Thy1-CFP/COX8A)C1Lich | ||
|---|---|---|---|
| Allele Name | transgenic insertion C1, Jeff W Lichtman | ||
| Common Name(s) | Thy1-CFP-MitoC; | ||
| Mutation Made By | Rob Burgess, The Jackson Laboratory | ||
| Site of Expression | mitochondria; many neuronal populations including retinal cells and all motor axons; coronal brain sections show pattern of somatosensory cortex barrel morphology | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Expressed Gene | COX8A, cytochrome c oxidase subunit 8A (ubiquitous), human | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized donor mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published lineC, was subsequently established. [J:122259] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
CFP relatedCell Biology Research
Vesicular Trafficking
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cell Biology Research
Fluorescent Proteins
Neurobiology Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Misgeld T; Kerschensteiner M; Bareyre FM; Burgess RW; Lichtman JW. 2007. Imaging axonal transport of mitochondria in vivo. Nat Methods 4(7):559-561. [PubMed: 17558414] [J:122259]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J |
| Stock Number: | 007940 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a separate commercial license from Harvard University.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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