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| These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. This mutant mouse strain may be useful in studies of mitochondrial transport. | |||||||||||||||
- Description
- Phenotype
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Noncarrier (Female x Male) 14-APR-08 Species laboratory mouse Generation N5F2 (02-JAN-09) Donating Investigator IMR Colony, The Jackson Laboratory Description
These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations including retinal cells and in all motor axons. Coronal brain sections reveal a fluorescence pattern showing somatosensory cortex barrel morphology. Neuronal, mitochondrial and neuromuscular junction morphology appears normal in transgenic mice. Axonal mitochondrial density is similar to wildtype. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of mitochondrial transport.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBA mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published line C, was subsequently established. The mice were backcrossed for at least 5 generations to C57BL/6J.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Fall 2007; 507. Watching the Energy Flow in Neurons: Jackson Laboratory Scientist Helps Develop Two Fluorescent Mouse Strains.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Thy1-CFP/COX8A)C1Lich/0
Background Not Specified
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:122259)
- mice are viable and show no neurological abnormalities
This mouse can be used to support research in many areas including:
CFP relatedCell Biology Research
Vesicular Trafficking
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cell Biology Research
Fluorescent Proteins
Neurobiology Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-CFP/COX8A)C1Lich | ||
|---|---|---|---|
| Allele Name | transgenic insertion C1, Jeff W Lichtman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Thy1-CFP-MitoC; | ||
| Mutation Made By | Rob Burgess, The Jackson Laboratory | ||
| Site of Expression | mitochondria; many neuronal populations including retinal cells and all motor axons; coronal brain sections show pattern of somatosensory cortex barrel morphology | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Expressed Gene | COX8A, cytochrome c oxidase subunit 8A (ubiquitous), human | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Founder line C1 corresponds to the published line C. | ||
| Molecular Note | A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized donor mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published lineC, was subsequently established. [MGI Ref ID J:122259] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Misgeld T; Kerschensteiner M; Bareyre FM; Burgess RW; Lichtman JW. 2007. Imaging axonal transport of mitochondria in vivo. Nat Methods 4(7):559-561. [PubMed: 17558414] [MGI Ref ID J:122259]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. Mating System Hemizygote x Noncarrier (Female x Male) 14-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Thy1-CFP/COX8A)C1Lich
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Thy1-CFP/COX8A)C1Lich x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Thy1-CFP/COX8A)C1Lich
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Thy1-CFP/COX8A)C1Lich
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Thy1-CFP/COX8A)C1Lich x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Thy1-CFP/COX8A)C1Lich
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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