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| This strain, commonly known as B6.K1, has a slow Ped or Qa2negative phenotype. These mice be used for research in embryonic and post-natal development. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Major Histocompatibility Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donor Strain B6.AK-H2k; B6.A-H2-T18a H2 Haplotype b3 Generation N20F?+F2 (28-JUL-08) Appearance
black
Related Genotype: a/aImportant Note
This C57BL/6 strain is homozygous for the age related hearing loss mutation Cdh23ahl, which on this background causes progressive hearing loss with onset after 10 months of age.Description
The Qa2, or Ped, complex includes four similar genes and is correlated with the rate of preimplantation embryo development. Qa2positive mice are characterized by the fast Ped phenotype; Qa2negative mice, which do not express the QA2 antigen, are characterized by the slow Ped phenotype. This strain, commonly known as B6.K1, is slow Ped or Qa2negative. B6.K1 females have longer gestation times and smaller litter sizes than the control B6.K2 (Stock No. 007959) females. (Warner CM, 1993) At birth, B6.K1 pups are slighter lighter than B6.K2 pups, but they exhibit an accelerated growth, and by week 6 both male and female B6.K1 mice are significantly heavier than B6.K2 mice. (Watkins A, 2006) At 21 weeks of age, B6.K1 females exhibit elevated systolic blood pressure, and both sexes exhibit elevated serum angiotensin-converting enzyme (ACE) activity. (Watkins A, 2006)Development
This H2 congenic was developed in the mid-1970s by Dr. Lorraine Flaherty through intercross and backcross of the congenic strains, B6.A-H2-T18a and B6.AK-H2k. The H2b3 haplotype, commonly described as B6.K1, consists of Kb, Db, Qa2negative and Tlab. The control strain, (Stock No. 007959) B6.Cg-H2b4/FlaCmwJ (B6.K2), was generated in a similar fashion, and is Qa2positive. Both strains have been backcrossed to C57BL/6 for more than 20 generations. Mice from Dr. Flaherty's colony were sent to Dr. Carol Warner at Northeastern University in 1985 and maintained as sibling matings. The colony was donated to the Repository in 2007.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
|---|---|---|---|
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| Gene Symbol and Name | Ped, preimplantation embryo development | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | Qa-2 antigen; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Flaherty L. 1976. The Tla region of the mouse: identification of a new serologically defined locus, Qa-2. Immunogenetics 3:533-539. [MGI Ref ID J:12074]
Watkins A; Wilkins A; Osmond C; Warner CM; Comiskey M; Hanson M; Fleming TP. 2006. The influence of mouse Ped gene expression on postnatal development. J Physiol 571(Pt 1):211-20. [PubMed: 16269433] [MGI Ref ID J:125255]
Additional References
Exley GE; Warner CM. 1999. Selection in favor of the Ped fast haplotype occurs between mid-gestation and birth. Immunogenetics 49(7-8):653-9. [PubMed: 10369923] [MGI Ref ID J:56469]
McElhinny AS; Kadow N; Warner CM. 1998. The expression pattern of the Qa-2 antigen in mouse preimplantation embryos and its correlation with the Ped gene phenotype. Mol Hum Reprod 4(10):966-71. [PubMed: 9809678] [MGI Ref ID J:50837]
Tian Z; Xu Y; Warner CM. 1992. Removal of Qa-2 antigen alters the Ped gene phenotype of preimplantation mouse embryos. Biol Reprod 47(2):271-6. [PubMed: 1327207] [MGI Ref ID J:1737]
Warner CM; Brownell MS; Rothschild MF. 1991. Analysis of litter size and weight in mice differing in Ped gene phenotype and the Q region of the H-2 complex. J Reprod Immunol 19(3):303-13. [PubMed: 1865393] [MGI Ref ID J:11367]
Warner CM; Panda P; Almquist CD; Xu Y. 1993. Preferential survival of mice expressing the Qa-2 antigen. J Reprod Fertil 99(1):145-7. [PubMed: 8283431] [MGI Ref ID J:15841]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $89.60 Female or Male Homozygous for H2b3 *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $179.20 Homozygous for H2b3 x Homozygous for H2b3
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $116.50 Female or Male Homozygous for H2b3 *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $233.00 Homozygous for H2b3 x Homozygous for H2b3
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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| Important Note | This C57BL/6 strain is homozygous for the age related hearing loss mutation Cdh23ahl, which on this background causes progressive hearing loss with onset after 10 months of age. |
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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