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Genes & Alleles

Dvl1;   Dvl1tm1Awb;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Anthony Wynshaw-Boris,   UCSD Generation N50+N1F0 (26-FEB-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of MEF (mouse embryonic fibroblasts) derived from homozygotes. Homozygotes exhibit diminished social interaction behavior, do not barber or trim whiskers of cagemates, show subordinance in social dominance tests, do not sleep huddled together and do not build nests. Although mutants have an attenuated prepulse inhibition of acoustic and tactile startle (sensorimotor gating of the startle reflex), they do not exhibit any deficits in locomotor or cognitive function. This mutant mouse strain may be useful in studies of social behavior, sensorimotor gating, and possibly psychiatric disorders.

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt part of exon 2 and exons 3 and 4, encoding amino acids 131-225. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice, and then backcrossed to 129S6/SvEvTac for more than 50 generations. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation to establish the colony.

Mammalian Phenotype Terms assigned by genotype Dvl1tm1Awb/Dvl1tm1Awb         129S/SvEv behavior/neurological phenotype abnormal huddling behavior (MGI Ref ID J:42758) mice slept in scattered random patterns over a period of time, mutant mice tended to loosely huddle in various quandrants of the cage, whereas wild type littermates were more often observed huddled in the same quadrant abnormal nest building behavior (MGI Ref ID J:42758) mice built smaller nests than wild type littermates mutant mice were also observed spleeping on top of intact nestlet material whisker trimming (MGI Ref ID J:42758) mutant mice did not trim/barber their cagemates' whiskers or facial hair, whereas the majority of separately housed wild type littermates displayed whisker trimming behavior nervous system phenotype reduced sensorimotor gating (MGI Ref ID J:42758) attenuation of acoustic pre-pulse inhibition was exhibited with both acoustic and tactile startle hearing/vestibular/ear phenotype *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:100861) normal stereocilia orientation at E18.5

Gene & Allele Details

Allele Symbol		Dvl1tm1Awb
Allele Name		targeted mutation 1, Anthony Wynshaw-Boris
Common Name(s)		Dvl1-; Dvl1del131-225;
Mutation Made By		Anthony Wynshaw-Boris,   UCSD
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TC-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Dvl1, dishevelled, dsh homolog 1 (Drosophila)
Chromosome		4
Gene Common Name(s)		DVL; MGC54245; dvl-1;
Molecular Note		A neomycin selection cassette replaced a genomic fragment containing part of exon 2 and exons 3 and 4, which encode amino acids 131-225. Western blot analysis on embryonic fibroblasts derived from homozygous mice confirmed that no detectable protein wasproduced from this allele. [MGI Ref ID J:42758]

Control Information

	Control
	None Available
Considerations for Choosing Controls

Genotyping Protocols

Dvl1^tm1Awb

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying   Dvl1^tm1Awb allele

007969

B6.129S6-Dvl1tm1Awb/J

View Strains carrying   Dvl1^tm1Awb     (1 strain)

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects

Sensorineural Research

References

Selected Reference(s)

Lijam N; Paylor R; McDonald MP; Crawley JN; Deng CX; Herrup K ; Stevens KE ; Maccaferri G ; McBain CJ ; Sussman DJ ; Wynshaw-Boris A. 1997. Social interaction and sensorimotor gating abnormalities in mice lacking Dvl1. Cell 90(5):895-905. [PubMed: 9298901]  [MGI Ref ID J:42758]

Additional References

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Price and Supply Information

Strain Name:	129S-Dvl1tm1Awb/J
Stock Number:	007965

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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