Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N15+F1 (29-APR-08)   Donating Investigator Anthony Wynshaw-Boris,   UCSD

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of MEF (mouse embryonic fibroblasts) derived from homozygotes. Homozygotes exhibit diminished social interaction behavior, do not barber or trim whiskers of cagemates, show subordinance in social dominance tests, do not sleep huddled together and do not build nests. Although mutants have an attenuated prepulse inhibition of acoustic and tactile startle (sensorimotor gating of the startle reflex), they do not exhibit any deficits in locomotor or cognitive function. This mutant mouse strain may be useful in studies of social behavior, sensorimotor gating, and possibly psychiatric disorders.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt part of exon 2 and exons 3 and 4, encoding amino acids 131-225. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice, and then backcrossed to C57BL/6J for 15 generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Dvl1^tm1Awb allele

007965

129S-Dvl1tm1Awb/J

View Strains carrying   Dvl1^tm1Awb     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Dvl1^tm1Awb/Dvl1^tm1Awb

        129S/SvEv

• behavior/neurological phenotype
• abnormal huddling behavior (MGI Ref ID J:42758)
  • mice slept in scattered random patterns
  • over a period of time, mutant mice tended to loosely huddle in various quandrants of the cage, whereas wild-type littermates were more often observed huddled in the same quadrant
• abnormal nest building behavior (MGI Ref ID J:42758)
  • mice built smaller nests than wild-type littermates
  • mutant mice were also observed spleeping on top of intact nestlet material
• whisker trimming (MGI Ref ID J:42758)
  • mutant mice did not trim/barber their cagemates' whiskers or facial hair, whereas the majority of separately housed wild-type littermates displayed whisker trimming behavior
• nervous system phenotype
• reduced sensorimotor gating (MGI Ref ID J:42758)
  • attenuation of acoustic pre-pulse inhibition was exhibited with both acoustic and tactile startle
• hearing/vestibular/ear phenotype
• *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:100861)
  • normal stereocilia orientation at E18.5

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects

Sensorineural Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Dvl1tm1Awb
Allele Name		targeted mutation 1, Anthony Wynshaw-Boris
Allele Type		Targeted (knock-out)
Common Name(s)		Dvl1-; Dvl1del131-225;
Mutation Made By		Anthony Wynshaw-Boris,   UCSD
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TC-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Dvl1, dishevelled, dsh homolog 1 (Drosophila)
Chromosome		4
Gene Common Name(s)		DVL; MGC54245; dvl-1;
Molecular Note		A neomycin selection cassette replaced a genomic fragment containing part of exon 2 and exons 3 and 4, which encode amino acids 131-225. Western blot analysis on embryonic fibroblasts derived from homozygous mice confirmed that no detectable protein wasproduced from this allele. [MGI Ref ID J:42758]

Genotyping

Genotyping Information

Genotyping Protocols

Dvl1^tm1Awb, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Lijam N; Paylor R; McDonald MP; Crawley JN; Deng CX; Herrup K ; Stevens KE ; Maccaferri G ; McBain CJ ; Sussman DJ ; Wynshaw-Boris A. 1997. Social interaction and sensorimotor gating abnormalities in mice lacking Dvl1. Cell 90(5):895-905. [PubMed: 9298901]  [MGI Ref ID J:42758]

Additional References

Dvl1^tm1Awb related

Ahmad-Annuar A; Ciani L; Simeonidis I; Herreros J; Fredj NB; Rosso SB; Hall A; Brickley S; Salinas PC. 2006. Signaling across the synapse: a role for Wnt and Dishevelled in presynaptic assembly and neurotransmitter release. J Cell Biol 174(1):127-39. [PubMed: 16818724]  [MGI Ref ID J:111190]

Hamblet NS; Lijam N; Ruiz-Lozano P; Wang J; Yang Y; Luo Z; Mei L; Chien KR; Sussman DJ; Wynshaw-Boris A. 2002. Dishevelled 2 is essential for cardiac outflow tract development, somite segmentation and neural tube closure. Development 129(24):5827-38. [PubMed: 12421720]  [MGI Ref ID J:79834]

Long JM; LaPorte P; Paylor R; Wynshaw-Boris A. 2004. Expanded characterization of the social interaction abnormalities in mice lacking Dvl1. Genes Brain Behav 3(1):51-62. [PubMed: 14960015]  [MGI Ref ID J:101889]

Purro SA; Ciani L; Hoyos-Flight M; Stamatakou E; Siomou E; Salinas PC. 2008. Wnt regulates axon behavior through changes in microtubule growth directionality: a new role for adenomatous polyposis coli. J Neurosci 28(34):8644-54. [PubMed: 18716223]  [MGI Ref ID J:138829]

Rosso SB; Sussman D; Wynshaw-Boris A; Salinas PC. 2005. Wnt signaling through Dishevelled, Rac and JNK regulates dendritic development. Nat Neurosci 8(1):34-42. [PubMed: 15608632]  [MGI Ref ID J:95820]

Wang J; Hamblet NS; Mark S; Dickinson ME; Brinkman BC; Segil N; Fraser SE; Chen P; Wallingford JB; Wynshaw-Boris A. 2006. Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation. Development 133(9):1767-78. [PubMed: 16571627]  [MGI Ref ID J:108512]

Wang J; Mark S; Zhang X; Qian D; Yoo SJ; Radde-Gallwitz K; Zhang Y; Lin X; Collazo A; Wynshaw-Boris A; Chen P. 2005. Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathway. Nat Genet 37(9):980-5. [PubMed: 16116426]  [MGI Ref ID J:100861]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.