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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;CBA-Tg(H/Olfr16-taumRFP,H/Olfr16-tauGFP)8Mom/MomJ (Changed: 10-JAN-11 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
This transgenic strain encodes two co-injected transgenes that both have 9 equally spaced repeats of the homeodomain binding sequence linked to the 405 base pairs of regulatory sequence just upstream of the transcription start site for Olfr16 driving expression of the full coding sequence of Olfr16 and either an IRES-tauGFP or an IRES-tauRFP just after the stop codon resulting in bicistronic expression of a fluorescent marker with the transgenically expressed olfactory receptor. Similar to the findings in strains bearing just one construct (see stocks #007979, #007980 and #007981) a massive increase is found in the number of cells that express the transgenic olfactory receptor, relative to the endogenous expression pattern, but the pattern of expression remains predominantly in the dorsal main olfactory epithelium. Expression of the co-injected transgenes is mutually exclusive with red and green fluorescence not found to overlap, proving that, despite an increase in the number of cells expressing these transgenes, only one olfactory receptor is expressed per olfactory sensory neuron.Development
This transgenic was generated by pronuclear co-injection of the two constructs into (C57BL/6J x CBA)F2 and was maintained on this segregating background until sperm was cryopreserved from hemizygous males in 2007.
Related Strains
Strains carrying other alleles of GFP
View Strains carrying other alleles of GFP (314 strains)
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:Tg(H/Olfr16-taumRFP,-tauGFP)8Mom related
Developmental Biology Research
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(H/Olfr16-taumRFP,-tauGFP)8Mom | ||
|---|---|---|---|
| Allele Name | transgene insertion 8, Peter Mombaerts | ||
| Allele Type | Transgenic (random, expressed) | ||
| Strain of Origin | (C57BL/6J x CBA)F2 | ||
| Site of Expression | GFP and RFP are expressed in olfactory sensory neurons. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | Olfr16, olfactory receptor 16, mouse, laboratory | ||
| Molecular Note | A construct comprised of a stretch of 9 equally spaced repeats of the homeodomain binding sequence upstream of a minigene with 405 base pairs upstream of the transcription start site of Olfr16, an intact Olfr16 coding sequence, and an IRES-tauGFP cassette just after the stop codon along with a second construct identical except for the substitute of IRES-taumRFP were coinjected into (C57BL/6J x CBA)F2 pronuclei. [MGI Ref ID J:166885] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Vassalli A; Feinstein P; Mombaerts P. 2010. Homeodomain binding motifs modulate the probability of odorant receptor gene choice in transgenic mice. Mol Cell Neurosci :. [PubMed: 21111823] [MGI Ref ID J:166885]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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