Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129P2-Sorl1Gt(pGT1tfr)1Ucd/J    (Changed: 22-AUG-08 ) Type Congenic; Gene Trap; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7+F1pN1   Donating Investigator David Hui,   University of Cincinnati - GRI

Description
Mice that are homozygous for the gene-trapped allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The gene trap contains the reporter gene beta-geo and has been inserted into exon 47. The donating investigator reports that one year old mutant mice exhibit a higher incidence of diffuse immunoreactive beta-amyloid deposits when compared to wildtype littermate controls. This mutant mouse strain may be useful in studies of Alzheimer's disease.

Development
A gene trap vector, pGT1tfr, was electroporated into 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for 6 generations before arriving at The Jackson Laboratory. The mice were then backcrossed to C57BL/6J for one generation.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Sorl1^Gt(Ex255)Byg/Sorl1^Gt(Ex255)Byg

        B6.129P2-Sorl1^Gt(Ex255)Byg

• nervous system phenotype
• amyloid beta deposits (MGI Ref ID J:125747)
  • at 1 year, mutants show higher incidence of diffuse beta-amyloid deposits in brain than found in wild-type littermates
• other phenotype
• amyloid beta deposits (MGI Ref ID J:125747)
  • at 1 year, mutants show higher incidence of diffuse beta-amyloid deposits in brain than found in wild-type littermates

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease

Genes & Alleles

Gene & Allele Information

Allele Symbol		Sorl1Gt(Ex255)Byg
Allele Name		gene trap Ex255, BayGenomics
Allele Type		Gene trapped
Common Name(s)		LR-11; Sorl1Gt(pGT1tfr)1Ucd;
Strain of Origin		129P2/OlaHsd
ES Cell Line Strain		129
Gene Symbol and Name		Sorl1, sortilin-related receptor, LDLR class A repeats-containing
Chromosome		9
Gene Common Name(s)		2900010L19Rik; AI596264; AW261561; C11orf32; FLJ21930; FLJ39258; LR11; LRP9; RGD619914; RIKEN cDNA 2900010L19 gene; SORLA; SorLA-1; expressed sequence AI596264; expressed sequence AW261561; gp250; mSorLA;
Molecular Note		A gene trap vector, pGT1tfr, was electroporated into 129P2/OlaHsd derived embryonic stem (ES) cells. [MGI Ref ID J:125747]

Genotyping

Genotyping Information

Genotyping Protocols

Sorl1^{Gt(pGT1tfr)1Ucd}, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Hui D; Greer T; Kodvawala A. 2007. Generation and description of gene trap mutation of Sorl1 MGI Direct Data Submission :.  [MGI Ref ID J:125747]

Additional References

Sorl1^Gt(Ex255)Byg related

Rohe M; Carlo AS; Breyhan H; Sporbert A; Militz D; Schmidt V; Wozny C; Harmeier A; Erdmann B; Bales KR; Wolf S; Kempermann G; Paul SM; Schmitz D; Bayer TA; Willnow TE; Andersen OM. 2008. Sortilin-related receptor with A-type repeats (SORLA) affects the amyloid precursor protein-dependent stimulation of ERK signaling and adult neurogenesis. J Biol Chem 283(21):14826-34. [PubMed: 18362153]  [MGI Ref ID J:137577]

Stryke D; Kawamoto M; Huang CC; Johns SJ; King LA; Harper CA; Meng EC; Lee RE; Yee A; L'Italien L; Chuang PT; Young SG; Skarnes WC; Babbitt PC; Ferrin TE. 2003. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res 31(1):278-81. [PubMed: 12520002]  [MGI Ref ID J:91812]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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