Strain Name:

129S-Dvl2tm1Awb/J

Stock Number:

008001

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Availability:

Cryopreserved - Ready for recovery

Half of the mice that are homozygous for this targeted mutation exhibit a perinatal lethal phenotype. This mutant mouse strain may be useful in studies of bone and cardiac development, neural tube closure and spina bifida.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN50+N1F1pN1
Generation Definitions
 
Donating InvestigatorDr. Anthony Wynshaw-Boris,   Case Western Reserve University, School of Medicine

Description
Half of homozygotes exhibit a perinatal lethal phenotype. Surviving homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Some newborn homozygotes fail to survive and exhibit breathing difficulties, cyanosis and reduced mobility. No gene product (protein) is detected by Western blot analysis of brain lystates. Some nonviable homozygotes display cardiac abnormalities. Most homozygotes (90%) have mild abnormalities of the ribs and vertebrae. 2 to 3% of the homozygous embryos display thoracic spina bifida and exencephaly. This mutant mouse strain may be useful in studies of bone and cardiac development, neural tube closure and spina bifida.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt part of exon 2 and exons 3 through 6. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice for more than 50 generations. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Transposition of the Great Arteries, Dextro-Looped 1; DTGA1
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Dvl2tm1Awb/Dvl2tm1Awb

        either: 129S6/SvEvTac-Dvl2tm1Awb or (involves: 129S6/SvEvTac * NIH Swiss)
  • mortality/aging
  • partial perinatal lethality
    • incomplete penetrance; more than 50% die after E18.5   (MGI Ref ID J:79834)
  • cardiovascular system phenotype
  • double outlet right ventricle
    • most common defect observed by mice with cardiovascular abnormalities   (MGI Ref ID J:79834)
    • frequently observed in conjuction with ventricular septal defects   (MGI Ref ID J:79834)
  • persistent truncus arteriosis
    • exhibited by several embryos   (MGI Ref ID J:79834)
  • transposition of great arteries
    • observed in a single embryo   (MGI Ref ID J:79834)
  • ventricular septal defect
    • frequently observed in conjuction with double outlet right ventricle   (MGI Ref ID J:79834)
  • embryogenesis phenotype
  • abnormal somite development
    • mild abnormalities in somite segmentation   (MGI Ref ID J:79834)
  • open neural tube
    • exhibited at E9.5 by 2-3% of mutant mice   (MGI Ref ID J:79834)
  • homeostasis/metabolism phenotype
  • cyanosis
    • surviving mice were predominantly female   (MGI Ref ID J:79834)
  • limbs/digits/tail phenotype
  • kinked tail
    • exhibited by 25% of surviving mice   (MGI Ref ID J:79834)
  • vestigial tail
    • exhibited by some surviving mice   (MGI Ref ID J:79834)
  • skeleton phenotype
  • abnormal rib morphology
    • 90% displayed minor abnormalities   (MGI Ref ID J:79834)
    • normal rib number   (MGI Ref ID J:79834)
    • rib bifurcation   (MGI Ref ID J:79834)
    • rib fusion   (MGI Ref ID J:79834)
  • abnormal vertebrae morphology
    • 90% displayed minor abnormalities   (MGI Ref ID J:79834)
    • defects localized dorsally in vertebral ribs and vertebral bodies   (MGI Ref ID J:79834)
    • abnormal thoracic vertebrae morphology
      • disorganized thoracic vertebrae   (MGI Ref ID J:79834)
  • abnormal xiphoid process morphology
    • observed in one neonate   (MGI Ref ID J:79834)
    • affecting the sixth sternebra   (MGI Ref ID J:79834)
  • scoliosis
    • exhibited by surviving mutant mice   (MGI Ref ID J:79834)
  • nervous system phenotype
  • exencephaly
    • exhibited at E9.5 by 2-3% of mutant mice   (MGI Ref ID J:79834)
  • open neural tube
    • exhibited at E9.5 by 2-3% of mutant mice   (MGI Ref ID J:79834)
  • hearing/vestibular/ear phenotype
  • *normal* hearing/vestibular/ear phenotype
    • normal stereocilia orientation at E18.5   (MGI Ref ID J:100861)

Dvl2tm1Awb/Dvl2tm1Awb

        involves: 129S6/SvEvTac
  • mortality/aging
  • partial perinatal lethality
    • 50% of mice die perinatally   (MGI Ref ID J:142392)
    • however, expression of a Dvl3 transgene can rescue perinatal lethality   (MGI Ref ID J:142392)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      heart
Neural Tube Defects
Perinatal Lethality
      Homozygous
Skeletal Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dvl2tm1Awb
Allele Name targeted mutation 1, Anthony Wynshaw-Boris
Allele Type Targeted (Null/Knockout)
Common Name(s) Dvl2-;
Mutation Made ByDr. Anthony Wynshaw-Boris,   Case Western Reserve University, School of Medicine
Strain of Origin129S6/SvEvTac
ES Cell Line NameTC1/TC-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Dvl2, dishevelled 2, dsh homolog (Drosophila)
Chromosome 11
Gene Common Name(s) Dvl-2;
Molecular Note A neomycin selection cassette inserted by homologous recombination replaced a portion of exon 2 and all of exons 3 through 6. Western blot analysis of homozygous mutant brain lysates indicated an absence of protein. [MGI Ref ID J:79834]

Genotyping

Genotyping Information

Genotyping Protocols

Dvl2tm1Awb, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hamblet NS; Lijam N; Ruiz-Lozano P; Wang J; Yang Y; Luo Z; Mei L; Chien KR; Sussman DJ; Wynshaw-Boris A. 2002. Dishevelled 2 is essential for cardiac outflow tract development, somite segmentation and neural tube closure. Development 129(24):5827-38. [PubMed: 12421720]  [MGI Ref ID J:79834]

Additional References

Dvl2tm1Awb related

Chen J; Stahl A; Krah NM; Seaward MR; Dennison RJ; Sapieha P; Hua J; Hatton CJ; Juan AM; Aderman CM; Willett KL; Guerin KI; Mammoto A; Campbell M; Smith LE. 2011. Wnt signaling mediates pathological vascular growth in proliferative retinopathy. Circulation 124(17):1871-81. [PubMed: 21969016]  [MGI Ref ID J:189464]

Etheridge SL; Ray S; Li S; Hamblet NS; Lijam N; Tsang M; Greer J; Kardos N; Wang J; Sussman DJ; Chen P; Wynshaw-Boris A. 2008. Murine dishevelled 3 functions in redundant pathways with dishevelled 1 and 2 in normal cardiac outflow tract, cochlea, and neural tube development. PLoS Genet 4(11):e1000259. [PubMed: 19008950]  [MGI Ref ID J:142392]

Glasco DM; Sittaramane V; Bryant W; Fritzsch B; Sawant A; Paudyal A; Stewart M; Andre P; Cadete Vilhais-Neto G; Yang Y; Song MR; Murdoch JN; Chandrasekhar A. 2012. The mouse Wnt/PCP protein Vangl2 is necessary for migration of facial branchiomotor neurons, and functions independently of Dishevelled. Dev Biol 369(2):211-22. [PubMed: 22771245]  [MGI Ref ID J:187622]

Gruber PJ; Epstein JA. 2004. Development gone awry: congenital heart disease. Circ Res 94(3):273-83. [PubMed: 14976138]  [MGI Ref ID J:96661]

Kioussi C; Briata P; Baek SH; Rose DW; Hamblet NS; Herman T; Ohgi KA; Lin C; Gleiberman A; Wang J; Brault V; Ruiz-Lozano P; Nguyen HD; Kemler R; Glass CK; Wynshaw-Boris A; Rosenfeld MG. 2002. Identification of a Wnt/Dvl/beta-Catenin --> Pitx2 Pathway Mediating Cell-Type-Specific Proliferation during Development. Cell 111(5):673-85. [PubMed: 12464179]  [MGI Ref ID J:80691]

Metcalfe C; Ibrahim AE; Graeb M; de la Roche M; Schwarz-Romond T; Fiedler M; Winton DJ; Corfield A; Bienz M. 2010. Dvl2 Promotes Intestinal Length and Neoplasia in the ApcMin Mouse Model for Colorectal Cancer. Cancer Res 70(16):6629-38. [PubMed: 20663899]  [MGI Ref ID J:162767]

Sinha T; Wang B; Evans S; Wynshaw-Boris A; Wang J. 2012. Disheveled mediated planar cell polarity signaling is required in the second heart field lineage for outflow tract morphogenesis. Dev Biol 370(1):135-44. [PubMed: 22841628]  [MGI Ref ID J:188039]

Wang J; Hamblet NS; Mark S; Dickinson ME; Brinkman BC; Segil N; Fraser SE; Chen P; Wallingford JB; Wynshaw-Boris A. 2006. Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation. Development 133(9):1767-78. [PubMed: 16571627]  [MGI Ref ID J:108512]

Wang J; Mark S; Zhang X; Qian D; Yoo SJ; Radde-Gallwitz K; Zhang Y; Lin X; Collazo A; Wynshaw-Boris A; Chen P. 2005. Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathway. Nat Genet 37(9):980-5. [PubMed: 16116426]  [MGI Ref ID J:100861]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes although half of the homozygotes have a perinatal lethal phenotype.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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