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Genes & Alleles

Pafah1b1;   Pafah1b1tm2Awb;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Anthony Wynshaw-Boris,   UCSD Generation N9+N1F1 (16-JUL-08)

Strain Description
These mice possess loxP sites flanking exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological analysis of brains from homozygotes reveals slightly wider CA1 and CA3 regions and a split in CA2 region in the hippocampus. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s).

Strain Development
A loxP site flanked targeting vector containing PGKneo was utilized in the construction of this mutant.

This selection cassette was inserted into intron 2 of the targeted gene, and another loxP site was inserted into intro 6. This construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice for 9 generations. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation to establish the colony.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Pafah1b1tm2Awb/Pafah1b1tm2Awb         involves: 129S/SvEv * FVB/N * NIH Black Swiss nervous system phenotype abnormal hippocampus morphology (MGI Ref ID J:49531) subtly abnormal

Gene & Allele Details

Allele Symbol		Pafah1b1tm2Awb
Allele Name		targeted mutation 2, Anthony Wynshaw-Boris
Common Name(s)		Lis1-loxP; Pafah1b1-loxP; Pafah1b1loxPex3-6;
Mutation Made By		Anthony Wynshaw-Boris,   UCSD
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TC-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Pafah1b1, platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit
Chromosome		11
Gene Common Name(s)		LIS-1; LIS1; LIS2; MDCR; MDS; Mdsh; Miller-Dieker syndrome homolog; PAF-AH 45; PAFAH; Pafaha; lissencephaly-1 protein; platelet-activating factor acetylhydrolase, alpha subunit;
Molecular Note		A floxed PGK-neo cassette was inserted into intron 2 and a third loxP site was introduced into intron 6 via homologous recombination. This allele is hypomorphic, because of disruption of transcription or splicing by the insertion of a phosphoglycerate kinase neomycin cassette from the targeting vector into the second intron (J:82268). [MGI Ref ID J:49531]

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Pafah1b1^tm2Awb

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (loxP-flanked Sequences)

Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

References

Selected Reference(s)

Hirotsune S; Fleck MW; Gambello MJ; Bix GJ; Chen A; Clark GD; Ledbetter DH; McBain CJ; Wynshaw-Boris A. 1998. Graded reduction of Pafah1b1 (Lis1) activity results in neuronal migration defects and early embryonic lethality [see comments] Nat Genet 19(4):333-9. [PubMed: 9697693]  [MGI Ref ID J:49531]

Additional References

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Price and Supply Information

Strain Name:	129S-Pafah1b1tm2Awb/J
Stock Number:	008002

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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