Strain Name: |
B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J |
|---|---|
Stock Number: |
008004 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
B6;SJL-Tg(Thy1-ECFP/Vamp2)1Sud/J (Changed: 02-MAY-08
) |
|
B6;SJL-Tg(Thy1-ECFP-Vamp2)1Sud/J (Changed: 13-MAR-08
) | |
|
B6SJL-Tg(Thy1-ECFP-Vamp2)1Sud/J (Changed: 12-MAR-08
) | |
| Genes & Alleles | CFP; Thy1; Vamp2; Tg(Thy1-ECFP/VAMP2)1Sud; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Thomas Sudhof, U. of Texas, SW Medical Center at Dallas Strain Description
Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical abnormalities. The mouse Thy1 gene promoter drives expression of a fusion protein made from ECFP and the bovine Vamp2 (Syb2) gene. Expression of the bovine gene is not eliminated. ECFP expression is neuron specific and signal may be found in various brain regions and neuromuscular junctions (NMJ).Strain Development
A transgenic vector incorporating an ECFP/bovine Vamp2 (Syb2) fusion protein directed by a mouse Thy1 promoter was created. Approximately 10 copies of the transgene were introduced to a mixed B6SJL (C57BL/6 x SJL) background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Thy1-ECFP/VAMP2)1Sud | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Thomas C Sudhof | ||
| Common Name(s) | BRO Syb2-ECFP; | ||
| Strain of Origin | C57BL/6 x SJL | ||
| Expressed Gene | Vamp2, vesicle-associated membrane protein 2, mouse, laboratory | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | A transgenic vector incorporating an ECFP/bovine Vamp2 (Syb2) fusion protein directed by a mouse Thy1 promoter was created. Approximately 10 copies of the transgene were introduced to a mixed C57BL/6 x SJL background. ECFP expression is neuron specific and signal may be found in various brain regions and neuromuscular junctions (NMJ). [J:131425] | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
006380 B6;129P2-Vamp2tm1Sud/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
CFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Sudhof TC. 2008. Generation of mice expressing a neuron-specific ECFP/Vamp2 fusion gene MGD Direct Data Submission :. [J:131425]
Price and Supply Information
| Strain Name: | B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J |
| Stock Number: | 008004 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license from The University of Texas Southwestern Medical Center at Dallas ("INSTITUTION") and other third parties prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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