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| These transgenic mice carry a T cell receptor transgene specific for human class II-associated invariant chain peptide (CLIP)/I-Ab complexes. On a RAG-deficient background, they exhibit diminished CD4+ T cell development as compared with two other transgenic mouse lines carrying T cell receptors selected on I-Ab. T cells from mice exhibit uniformly high Vbeta6 expression and strong proliferative responses to human CLIP (hCLIP). These mice are useful in studies of T cell development and function. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg-Tg(Tcra-V18-Tcra-J,Tcrb-V6-Tcrb-D-Tcrb-J)AR206Ayr/J (Changed: 14-NOV-07 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1
Generation DefinitionsDonating Investigator Alexander Rudensky, University of Washington Description
Mice homozygous for this transgene are viable and fertile and do not display any gross physical or behavioral abnormalities. These transgenic mice carry a T cell receptor transgene specific for human class II-associated invariant chain peptide (CLIP)/I-Ab complexes. On a RAG-deficient background, they exhibit diminished CD4+ T cell development as compared with two other transgenic mouse lines carrying T cell receptors selected on I-Ab. T cells from mice exhibit uniformly high Vbeta6 expression and strong proliferative responses to human CLIP (hCLIP). These mice are useful in studies of T cell development and function.Development
Fragments of Valpha18 (P14A.1)-Jalpha45 and Vbeta6-Dbeta1-Jbeta2.2 were PCR-amplified from genomic DNA of hCLIP:I-Ab-specific T cell hybridoma 51-11.5 grown in H2b (C57BL/6J) mice. The amplified fragments were inserted into separate vectors co-injected into (C57BL/6 x DBA/2)F2 embryos. Mouse -derived T cell receptor promoters drive expression. This founder line (AR206), carrying co-integrated transgenes, was backcrossed more than 15 times to C57BL/6.
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (45 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/0
involves: C57BL/6 * DBA/2
- hematopoietic system phenotype
- abnormal positive T cell selection
- positive selection of CD4+ T cells is significantly reduced in the thymus compared to other lines (MGI Ref ID J:125548)
- immune system phenotype
- abnormal positive T cell selection
- positive selection of CD4+ T cells is significantly reduced in the thymus compared to other lines (MGI Ref ID J:125548)
- cellular phenotype
- abnormal positive T cell selection
- positive selection of CD4+ T cells is significantly reduced in the thymus compared to other lines (MGI Ref ID J:125548)
This mouse can be used to support research in many areas including:
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr | ||
|---|---|---|---|
| Allele Name | transgene insertion AR206, Alexander Y Rudensky | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TCli TCR; Tg(Tcra-V18-Tcra-J, Tcrb-V6-Tcrb-D-Tcrb-J)AR206Ayr; | ||
| Mutation Made By | Alexander Rudensky, University of Washington | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Molecular Note | Fragments of Valpha18 (P14A.1)-Jalpha45 and Vbeta6-Dbeta1-Jbeta2.2 were PCR-amplified from genomic DNA of hCLIP:I-Ab-specific T cell hybridoma 51-11.5 grown in H2b (C57BL/6J) mice. The amplified fragments were inserted into separate vectors and co-injected into (C57BL/6 x DBA/2)F2 embryos. Mouse -derived T cell receptor promoters (Tcra, Tcrb) drive expression. This founder line (AR206), carrying co-integrated transgenes, was backcrossed more than 15 times to C57BL/6. [MGI Ref ID J:125548] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wong P; Goldrath AW; Rudensky AY. 2000. Competition for specific intrathymic ligands limits positive selection in a TCR transgenic model of CD4+ T cell development. J Immunol 164(12):6252-9. [PubMed: 10843678] [MGI Ref ID J:125548]
Additional References
Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr relatedBurchill MA; Yang J; Vang KB; Moon JJ; Chu HH; Lio CW; Vegoe AL; Hsieh CS; Jenkins MK; Farrar MA. 2008. Linked T cell receptor and cytokine signaling govern the development of the regulatory T cell repertoire. Immunity 28(1):112-21. [PubMed: 18199418] [MGI Ref ID J:131384]
Hsieh CS; Zheng Y; Liang Y; Fontenot JD; Rudensky AY. 2006. An intersection between the self-reactive regulatory and nonregulatory T cell receptor repertoires. Nat Immunol 7(4):401-10. [PubMed: 16532000] [MGI Ref ID J:112554]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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