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| These transgenic mice carry a T cell receptor transgene specific for human class II-associated invariant chain peptide (CLIP)/I-Ab complexes. On a RAG-deficient background, they exhibit diminished CD4+ T cell development as compared with two other transgenic mouse lines carrying T cell receptors selected on I-Ab. T cells from mice exhibit uniformly high Vbeta6 expression and strong proliferative responses to human CLIP (hCLIP). These mice are useful in studies of T cell development and function. | |||||||||
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Description
Strain Information
Former Names B6.Cg-Tg(Tcra-V18-Tcra-J,Tcrb-V6-Tcrb-D-Tcrb-J)AR206Ayr/J (Changed: 14-NOV-07 ) Type Mutant Strain; Transgenic; Species laboratory mouse Donating Investigator Alexander Rudensky, University of Washington Description
Mice homozygous for this transgene are viable and fertile and do not display any gross physical or behavioral abnormalities. These transgenic mice carry a T cell receptor transgene specific for human class II-associated invariant chain peptide (CLIP)/I-Ab complexes. On a RAG-deficient background, they exhibit diminished CD4+ T cell development as compared with two other transgenic mouse lines carrying T cell receptors selected on I-Ab. T cells from mice exhibit uniformly high Vbeta6 expression and strong proliferative responses to human CLIP (hCLIP). These mice are useful in studies of T cell development and function.Development
Fragments of Valpha18 (P14A.1)-Jalpha45 and Vbeta6-Dbeta1-Jbeta2.2 were PCR-amplified from genomic DNA of hCLIP:I-Ab-specific T cell hybridoma 51-11.5 grown in H2b (C57BL/6J) mice. The amplified fragments were inserted into separate vectors co-injected into (C57BL/6 x DBA/2)F2 embryos. Mouse -derived T cell receptor promoters drive expression. This founder line (AR206), carrying co-integrated transgenes, was backcrossed more than 15 times to C57BL/6.
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (35 strains)
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/0
involves: C57BL/6 * DBA/2
- hematopoietic system phenotype
- abnormal positive T cell selection (MGI Ref ID J:125548)
- positive selection of CD4+ T cells is significantly reduced in the thymus compared to other lines
- immune system phenotype
- abnormal positive T cell selection (MGI Ref ID J:125548)
- positive selection of CD4+ T cells is significantly reduced in the thymus compared to other lines
Research Applications
This mouse can be used to support research in many areas including:
Tcra relatedImmunology and Inflammation Research
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr | ||
|---|---|---|---|
| Allele Name | transgene insertion AR206, Alexander Y Rudensky | ||
| Common Name(s) | TCli TCR; Tg(Tcra-V18-Tcra-J, Tcrb-V6-Tcrb-D-Tcrb-J)AR206Ayr; | ||
| Mutation Made By | Alexander Rudensky, University of Washington | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Promoter | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| Molecular Note | Fragments of Valpha18 (P14A.1)-Jalpha45 and Vbeta6-Dbeta1-Jbeta2.2 were PCR-amplified from genomic DNA of hCLIP:I-Ab-specific T cell hybridoma 51-11.5 grown in H2b (C57BL/6J) mice. The amplified fragments were inserted into separate vectors and co-injected into (C57BL/6 x DBA/2)F2 embryos. Mouse -derived T cell receptor promoters (Tcra, Tcrb) drive expression. This founder line (AR206), carrying co-integrated transgenes, was backcrossed more than 15 times to C57BL/6. [MGI Ref ID J:125548] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Wong P; Goldrath AW; Rudensky AY. 2000. Competition for specific intrathymic ligands limits positive selection in a TCR transgenic model of CD4+ T cell development. J Immunol 164(12):6252-9. [PubMed: 10843678] [MGI Ref ID J:125548]
Additional References
Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr related
Burchill MA; Yang J; Vang KB; Moon JJ; Chu HH; Lio CW; Vegoe AL; Hsieh CS; Jenkins MK; Farrar MA. 2008. Linked T cell receptor and cytokine signaling govern the development of the regulatory T cell repertoire. Immunity 28(1):112-21. [PubMed: 18199418] [MGI Ref ID J:131384]Hsieh CS; Zheng Y; Liang Y; Fontenot JD; Rudensky AY. 2006. An intersection between the self-reactive regulatory and nonregulatory T cell receptor repertoires. Nat Immunol 7(4):401-10. [PubMed: 16532000] [MGI Ref ID J:112554]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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