Strain Name: |
STOCK Cd44tm1Ugu/J |
|---|---|
Stock Number: |
008008 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
SJL.129-Cd44tm1Ugu/J (Changed: 06-JUN-08
) |
| Genes & Alleles | Cd44; Cd44tm1Ugu; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Ursula Gunthert, University of Basel Generation N10+ (02-NOV-07) Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice possess loxP sites on either side of exon 6 of the targeted gene. RT-PCR analysis reveals that exon 7 is not expressed in the floxed mice, and Southern blots confirm that 1.6kb of exon 7 was deleted during homologous recombination. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 6 and exon 7 deleted in the cre-expressing tissue(s). According to the donating investigator, homozygotes exhibit reduced incidence and severity of induced experimental autoimmune encephalomyelitis (EAE) when compared to wildtype controls. Please note that on a 129/Sv genetic background, homozygotes are resistant to trinitrobenzene sulfonic acid (TNBS)-induced colitis.This mutant mouse strain may be useful in studies of multiple sclerosis (MS) and inflammatory diseases.
Strain Development
A targeting vector containing a loxP site-flanked neomycin resistance cassette inserted upstream and a third loxP site inserted downstream of exon 6 of the targeted gene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Approximately 1.6kb of exon 7 also was deleted from the targeted gene during homologous recombination. The resulting chimeric mice were bred to SJL mice (from Erasmus Medical Center, Rotterdam, The Netherlands) for 10 generations prior to arrival at The Jackson Laboratory.NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 4 loci that were homozygous for markers other than SJL/J. These loci are on chromosome 3 (approximately 16.3 cM), chromosome 9 (approximately 6.6 cM), chromosome 11 (approximately 9.8 cM), and chromosome 18 (approximately 26.2 cM). A possible source of this contamination could be from the SJL substrain used for backcrossing.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Cd44tm1Ugu | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ursula Gunthert | ||
| Common Name(s) | CD44v7-; | ||
| Mutation Made By | Ursula Gunthert, University of Basel | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Cd44, CD44 antigen | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AU023126; AW121933; AW146109; CD44A; CDW44; CSPG8; ECMR-III; HCELL; HERMES; IN; LHR; Ly-24; MC56; MDU2; MDU3; METAA; MGC10468; MGC124941; MIC4; MUTCH-I; Pgp-1; Pgp1; RHAMM; expressed sequence AU023126; expressed sequence AW121933; expressed sequence AW146109; lymphocyte antigen 24; phagocyte glycoprotein 1; | ||
| Molecular Note | A loxP site was inserted 3' of exon v6 while a neomycin resistance cassette with a 5' loxP site was inserted 5' of exon v6. During insertion, 1.6 kb of sequence was lost in the exon v7 region leading to deficient expression of this variant in homozygous mutant mice. [MGI Ref ID J:62867] | ||
Control Information
| Control | ||
|---|---|---|
| 000686 SJL/J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Cd44tm1Ugu
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Cd44
005085 B6.Cg-Cd44tm1Hbg/J 005878 NOD.Cg-Cd44tm1Hbg/J 003899 STOCK Cd44tm1Hbg/J View Strains carrying other alleles of Cd44 (3 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Autoimmunity (experimental allergic encephalomyelitis (EAE))
Research Tools
Cre-lox System (loxP-flanked Sequences)
References
Selected Reference(s)
Additional ReferencesWittig BM; Johansson B; Zoller M; Schwarzler C; Gunthert U. 2000. Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7). J Exp Med 191(12):2053-64. [PubMed: 10859330] [MGI Ref ID J:62867]
Price and Supply Information
| Strain Name: | STOCK Cd44tm1Ugu/J |
| Stock Number: | 008008 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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