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| These Cx43flox mutant mice may be useful in generating conditional mutations for studying the role of connexin and gap junctions in various tissues and systems, including the cardiovascular system. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Brian Duling, Univ. of Virginia School of Medicine Description
Mice homozygous for this Cx43flox conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. Presence of the loxP sites has no reported affect on expression of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). These Cx43flox mutant mice may be useful in generating conditional mutations for studying the role of connexin and gap junctions in various tissues and systems, including the cardiovascular system.Development
A targeting vector was designed to place a loxP site just upstream of, and a loxP-flanked neo/TK cassette just downstream of exon 2 of the targeted gene. This construct was electroporated into 129S7/SvEvBrd-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre expressing plasmid. The resulting ES cells with the selection cassette removed and a single loxP site remaining on each side of exon 2 (called "type II deletion: conditional mutation" in the primary publication) were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 mice to generate Cx43flox mutant mice. These mice were backcrossed to C57BL/6NTac using a speed congenic approach and determined to be congenic on this background prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gja1
002201 B6;129S-Gja1tm1Kdr/J View Strains carrying other alleles of Gja1 (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gja1tm1Dlg/Gja1tm1Dlg
involves: 129S7/SvEvBrd * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:71092)
Research Applications
This mouse can be used to support research in many areas including:
Cardiovascular Research
Vascular Defects
Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gja1tm1Dlg | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Brian R Duling | ||
| Common Name(s) | VEC Cx43 KO; | ||
| Mutation Made By | Brian Duling, Univ. of Virginia School of Medicine | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1 | ||
| ES Cell Line Name | AB2.2 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1 | ||
| Gene Symbol and Name | Gja1, gap junction protein, alpha 1 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | AU042049; AW546267; CX43; Cx43alpha1; DFNB38; GJAL; Gja-1; MGC93610; ODDD; alpha 1 connexin; connexin 43; connexin43; expressed sequence AU042049; expressed sequence AW546267; | ||
| Molecular Note | LoxP sites were inserted flanking exon 2. A neomycin selection cassette and thymidine kinase gene that were also inserted 3' to the loxP sites were removed by transient cre recombinase expression in ES cells prior to the production of chimeric mice. Western blot and immunohistochemistry analysis on homozygous mice confirmed that there were no detectable changes in the expression of the encoded protein. [MGI Ref ID J:71092] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gja1tm1Dlg, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Calera MR; Topley HL; Liao Y; Duling BR; Paul DL; Goodenough DA. 2006. Connexin43 is required for production of the aqueous humor in the murine eye. J Cell Sci 119(Pt 21):4510-9. [PubMed: 17046998] [MGI Ref ID J:116081]
Liao Y; Day KH; Damon DN; Duling BR. 2001. Endothelial cell-specific knockout of connexin 43 causes hypotension and bradycardia in mice. Proc Natl Acad Sci U S A 98(17):9989-94. [PubMed: 11481448] [MGI Ref ID J:71092]
Additional References
Gja1tm1Dlg related
Casper KB; Jones K; McCarthy KD. 2007. Characterization of astrocyte-specific conditional knockouts. Genesis 45(5):292-9. [PubMed: 17457931] [MGI Ref ID J:121777]Liao Y; Regan CP; Manabe I; Owens GK; Day KH; Damon DN; Duling BR. 2007. Smooth muscle-targeted knockout of connexin43 enhances neointimal formation in response to vascular injury. Arterioscler Thromb Vasc Biol 27(5):1037-42. [PubMed: 17332489] [MGI Ref ID J:135088]
Parthasarathi K; Ichimura H; Monma E; Lindert J; Quadri S; Issekutz A; Bhattacharya J. 2006. Connexin 43 mediates spread of Ca2+-dependent proinflammatory responses in lung capillaries. J Clin Invest 116(8):2193-200. [PubMed: 16878174] [MGI Ref ID J:113107]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Gja1tm1Dlg *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Gja1tm1Dlg x Homozygous for Gja1tm1Dlg
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Gja1tm1Dlg *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Gja1tm1Dlg x Homozygous for Gja1tm1Dlg
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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