Strain Name:

CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J

Stock Number:

008040

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Availability:

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The Cre-inducible expression of DTR in these iDTR mutant mice render cells susceptible to ablation following Diphtheria toxin administration.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN5F4pN1
Generation Definitions
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.

When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. 011069), this mutant mouse strain may be useful in studies of metabolic dysfunction.

Of note, iDTR mice are also available on a C57BL/6 background (as Stock No. 007900) and a NOD congenic background (as Stock No. 016603).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. These iDTR mice were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory (as Stock No. 007900). Upon arrival, some mice were additionally backcrossed to BALB/cByJ (Stock No. 001026) to generate this congenic strain (Stock No. 008040).

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Gt(ROSA)26Sortm1(HBEGF)Awai allele
007900   C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
016603   NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ
View Strains carrying   Gt(ROSA)26Sortm1(HBEGF)Awai     (2 strains)

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
006067   129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006041   129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
013205   129S-Gt(ROSA)26Sortm1(NOTCH3)Sat/Mmjax
003310   129S-Gt(ROSA)26Sortm1Sor/J
013207   129S-Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat/Mmjax
009043   129S-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J
007844   129S4/SvJae-Gt(ROSA)26Sortm2(FLP*)Sor/J
003946   129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
017626   B6(Cg)-Gt(ROSA)26Sortm1(CAG-GFP/Eif2c2)Zjh/J
010633   B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J
024540   B6(Cg)-Gt(ROSA)26Sortm1(Sstr3/GFP)Bky/J
008242   B6(Cg)-Gt(ROSA)26Sortm4(Ikbkb)Rsky/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006071   B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
008463   B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J
008606   B6.129-Gt(ROSA)26Sortm1Joe/J
006080   B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006075   B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
011008   B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
017492   B6.129P2-Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
024708   B6.129P2-Gt(ROSA)26Sortm1(CAG-RABVgp4,-TVA)Arenk/J
009669   B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J
008513   B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J
013586   B6.129P2-Gt(ROSA)26Sortm1Nik/J
013587   B6.129P2-Gt(ROSA)26Sortm3Nik/J
022367   B6.129S4-Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a,-birA)Wtp/J
009086   B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
012930   B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J
009044   B6.129S4-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J
007743   B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
009673   B6.129S6(C)-Gt(ROSA)26Sortm3(HIF1A*)Kael/J
022626   B6.129S6(SJL)-Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Mmjax
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
017983   B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
021071   B6.Cg-Gt(ROSA)26Sortm1(CAG-PA-GFP)Rmpl/J
014588   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J
014602   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-mCherry)Eggn/J
023749   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007920   B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
012567   B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J
007903   B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
024109   B6.Cg-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
014648   B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ
021188   B6.Cg-Gt(ROSA)26Sortm40.1(CAG-aop3/EGFP)Hze/J
007906   B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
024179   B6;129-Gt(ROSA)26Sortm1(Actb-T,-GFP)Dalco/J
017455   B6;129-Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng/J
024857   B6;129-Gt(ROSA)26Sortm1(CAG-xstpx-cas9,-EGFP)Fezh/J
010527   B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J
016262   B6;129-Gt(ROSA)26Sortm1(Foxo1/GFP)Jke/J
017962   B6;129-Gt(ROSA)26Sortm1(RAC1*)Jkis/J
008883   B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ
004847   B6;129-Gt(ROSA)26Sortm1(cre/ERT)Nat/J
021025   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
008516   B6;129-Gt(ROSA)26Sortm1Joe/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
021847   B6;129-Gt(ROSA)26Sortm1Ytchn/J
008889   B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ
009253   B6;129-Gt(ROSA)26Sortm2Nat/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008886   B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ
010557   B6;129-Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat/J
021429   B6;129-Gt(ROSA)26Sortm4(CAG-GFP*)Nat/J
021039   B6;129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J
010523   B6;129P2-Gt(ROSA)26Sortm1(CAG-ALPP)Fawa/J
002073   B6;129S-Gt(ROSA)26Sor/J
018385   B6;129S-Gt(ROSA)26Sortm1(CAG-COX8A/Dendra2)Dcc/J
022516   B6;129S-Gt(ROSA)26Sortm1(Cdkn1c)Jfpa/J
013206   B6;129S-Gt(ROSA)26Sortm1(NOTCH3*R1031C)Sat/Mmjax
018397   B6;129S-Gt(ROSA)26Sortm1.1(CAG-COX8A/Dendra2)Dcc/J
023139   B6;129S-Gt(ROSA)26Sortm1.1Ksvo/J
012569   B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
012570   B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J
012735   B6;129S-Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze/J
014538   B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J
014539   B6;129S-Gt(ROSA)26Sortm39(CAG-hop/EYFP)Hze/J
021875   B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
021876   B6;129S-Gt(ROSA)26Sortm66.1(CAG-tdTomato)Hze/J
024105   B6;129S-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
024750   B6;129S4-Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc/J
023035   B6;129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
016999   B6;129S6-Gt(ROSA)26Sortm1(xstpx-rtTA2S*M2)Whsu/J
007908   B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007905   B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
024106   B6;129S6-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/J
019101   B6N.129S4(B6)-Gt(ROSA)26Sortm1Sor/CjDswJ
016226   B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J
019013   B6N.129S6(Cg)-Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
019016   B6N.129S6(Cg)-Gt(ROSA)26Sortm3(CAG-FLPo/ERT2)Alj/J
023537   B6N.129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
025701   B6N;129S1-Gt(ROSA)26Sortm1(Grem1)Svok/J
019120   BALB/c-Gt(ROSA)26Sortm10(Lmp1)Rsky/J
009670   C.129P2(B6)-Gt(ROSA)26Sortm1(DTA)Lky/J
008603   C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
002955   C.129S7-Gt(ROSA)26Sor/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J
012637   C57BL/6-Gt(ROSA)26Sortm5(Map3k14)Rsky/J
012638   C57BL/6-Gt(ROSA)26Sortm6(Map3k14*)Rsky/J
012343   C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J
012352   C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J
012361   C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
020458   C57BL/6N-Gt(ROSA)26Sortm13(CAG-MYC,-CD2*)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/GrsrJ
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
009427   FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
016977   FVB.129S6-Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
010920   FVB;129P2-Gt(ROSA)26Sortm1(birA)Mejr/J
013731   STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
010675   STOCK Gt(ROSA)26Sortm1(CAG-EGFP)Fsh/Mmjax
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
022793   STOCK Gt(ROSA)26Sortm1(LRRK2*R1441C)Djmo/J
008159   STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
011004   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
008600   STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
018999   STOCK Gt(ROSA)26Sortm1(tTA,tetO-Mir155)Fjsl/J
018998   STOCK Gt(ROSA)26Sortm1(tTA,tetO-Mir21)Fjsl/J
010701   STOCK Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax
022386   STOCK Gt(ROSA)26Sortm1.1(CAG-EGFP/Rpl10a,-birA)Wtp/J
024858   STOCK Gt(ROSA)26Sortm1.1(CAG-cas9,-EGFP)Fezh/J
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
025671   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(tetO-Fgf10)1Jaw/SpdlJ
024746   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Hprttm1(tetO-Dkk1)Spdl Tg(TCF/Lef1-lacZ)34Efu/J
010812   STOCK Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh/Mmjax
017922   STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J
023898   STOCK Gt(ROSA)26Sortm11.1(Setd5-GFP)Mgn/Mmjax
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
018906   STOCK Gt(ROSA)26Sortm3(CAG-FLPo/ERT2)Alj/J
013124   STOCK Gt(ROSA)26Sortm3(Gli3)Amc/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
009674   STOCK Gt(ROSA)26Sortm4(HIF2A*)Kael/J
024107   STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ
012266   STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J
017912   STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo/J
013123   STOCK Gt(ROSA)26Sortm6(Gli1)Amc/J
017921   STOCK Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo/J
017909   STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo/J
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (162 strains)

Strains carrying other alleles of HBEGF
014176   C57BL/6-Tg(CLEC4C-HBEGF)956Cln/J
View Strains carrying other alleles of HBEGF     (1 strain)

Additional Web Information

Information about the Rosa26 locus on the Soriano lab web page

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Cd19tm1(cre)Cgn/? Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai

        involves: 129P2/OlaHsd * C57BL/6   (conditional)
  • immune system phenotype
  • abnormal lymph node cell ratio
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the lymph nodes   (MGI Ref ID J:131076)
  • abnormal lymphocyte morphology
    • after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice   (MGI Ref ID J:131076)
    • abnormal B cell morphology
      • CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days   (MGI Ref ID J:131076)
      • absent immature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
      • absent mature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
    • abnormal lymphocyte cell number
      • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
      • absent immature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
      • absent mature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
  • abnormal splenic cell ratio
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen   (MGI Ref ID J:131076)
  • hematopoietic system phenotype
  • abnormal bone marrow cell number
    • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
  • abnormal lymphocyte morphology
    • after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice   (MGI Ref ID J:131076)
    • abnormal B cell morphology
      • CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days   (MGI Ref ID J:131076)
      • absent immature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
      • absent mature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
    • abnormal lymphocyte cell number
      • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
      • absent immature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
      • absent mature B cells
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed   (MGI Ref ID J:131076)
  • abnormal splenic cell ratio
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen   (MGI Ref ID J:131076)

Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+ Tg(Gh1-cre)bKnmn/0

        involves: C57BL/6 * FVB/N   (conditional)
  • homeostasis/metabolism phenotype
  • abnormal circulating hormone level   (MGI Ref ID J:169459)
    • decreased circulating growth hormone level
      • in diphtheria toxin-treated mice fed a high-fat or low-fat diet   (MGI Ref ID J:169459)
    • decreased circulating insulin level
      • in diphtheria toxin-treated mice when a high-fat diet or a low-fat diet   (MGI Ref ID J:169459)
    • decreased circulating insulin-like growth factor I level
      • in diphtheria toxin-treated mice fed a high-fat or low-fat diet   (MGI Ref ID J:169459)
    • increased circulating leptin level
      • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • abnormal energy expenditure   (MGI Ref ID J:169459)
    • decreased energy expenditure
      • in diphtheria toxin-treated mice fed a low-fat during the nocturnal phase or when mice are fed a high-fat diet   (MGI Ref ID J:169459)
  • abnormal gas homeostasis   (MGI Ref ID J:169459)
    • decreased oxygen consumption
      • in diphtheria toxin-treated mice fed a low-fat during the nocturnal phase   (MGI Ref ID J:169459)
    • increased respiratory quotient
      • in diphtheria toxin-treated mice fed a low-fat   (MGI Ref ID J:169459)
  • abnormal glucose homeostasis   (MGI Ref ID J:169459)
    • decreased circulating glucose level
      • in diphtheria toxin-treated mice when fed standard chow   (MGI Ref ID J:169459)
      • in diphtheria toxin-treated mice when fed a high-fat diet or a low-fat diet during an insulin tolerance test   (MGI Ref ID J:169459)
    • decreased circulating insulin level
      • in diphtheria toxin-treated mice when a high-fat diet or a low-fat diet   (MGI Ref ID J:169459)
    • impaired glucose tolerance
      • in diphtheria toxin-treated mice fed a high-fat   (MGI Ref ID J:169459)
    • increased insulin sensitivity
      • in diphtheria toxin-treated mice when fed standard chow, a high-fat diet, or a low-fat diet   (MGI Ref ID J:169459)
  • decreased liver triglyceride level
    • in diphtheria toxin-treated mice fed a high-fat   (MGI Ref ID J:169459)
  • endocrine/exocrine gland phenotype
  • decreased somatotroph cell number
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • small adenohypophysis
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • adipose tissue phenotype
  • increased retroperitoneal fat pad weight
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • increased subcutaneous adipose tissue amount
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • increased total body fat amount
    • in diphtheria toxin-treated mice fed standard chow or a high fat diet   (MGI Ref ID J:169459)
  • growth/size/body phenotype
  • decreased body weight
    • in diphtheria toxin-treated mice fed a high fat diet   (MGI Ref ID J:169459)
  • decreased lean body mass
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • increased total body fat amount
    • in diphtheria toxin-treated mice fed standard chow or a high fat diet   (MGI Ref ID J:169459)
  • liver/biliary system phenotype
  • decreased liver triglyceride level
    • in diphtheria toxin-treated mice fed a high-fat   (MGI Ref ID J:169459)
  • decreased liver weight
    • in diphtheria toxin-treated mice fed standard chow or a high fat diet   (MGI Ref ID J:169459)
  • nervous system phenotype
  • decreased somatotroph cell number
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • small adenohypophysis
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)
  • behavior/neurological phenotype
  • decreased fluid intake
    • in diphtheria toxin-treated mice fed a low-fat   (MGI Ref ID J:169459)
  • integument phenotype
  • increased subcutaneous adipose tissue amount
    • in diphtheria toxin-treated mice   (MGI Ref ID J:169459)

Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai Tg(Gdf9-cre)5092Coo/0

        involves: C57BL/6   (conditional)
  • reproductive system phenotype
  • abnormal ovary morphology   (MGI Ref ID J:157008)
    • abnormal ovarian follicle morphology
      • with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis   (MGI Ref ID J:157008)
      • abnormal granulosa cell morphology
        • cells have undergone apoptosis in preantral follicles   (MGI Ref ID J:157008)
  • oocyte degeneration
    • oocytes have undergone apoptosis in preantral follicles   (MGI Ref ID J:157008)
  • endocrine/exocrine gland phenotype
  • abnormal ovary morphology   (MGI Ref ID J:157008)
    • abnormal ovarian follicle morphology
      • with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis   (MGI Ref ID J:157008)
      • abnormal granulosa cell morphology
        • cells have undergone apoptosis in preantral follicles   (MGI Ref ID J:157008)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cardiovascular Research
      Cre-lox System
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gt(ROSA)26Sortm1(HBEGF)Awai
Allele Name targeted mutation 1, Ari Waisman
Allele Type Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence)
Common Name(s) R26iDTR; ROSA26-DTR; Rosa26-LSL-DTR; Rosa26iDTR; RosaiDTR; iDTR;
Mutation Made By Ari Waisman,   Johannes Gutenberg University of Mainz
Strain of OriginB6.Cg-Thy1
ES Cell Line NameBruce 4
ES Cell Line StrainB6.Cg-Thy1
Expressed Gene HBEGF, heparin-binding EGF-like growth factor, chimpanzee
General Note Phenotypic Similarity to Human Syndrome: Isolated Growth Hormone Deficiency, Adult Onset (J:169459)
Molecular Note A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. [MGI Ref ID J:131076]
 
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;

Genotyping

Genotyping Information

Genotyping Protocols

Gt(ROSA)26Sortm1(HBEGF)Awai, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920]  [MGI Ref ID J:131076]

Additional References

Gt(ROSA)26Sortm1(HBEGF)Awai related

Arruda-Carvalho M; Sakaguchi M; Akers KG; Josselyn SA; Frankland PW. 2011. Posttraining ablation of adult-generated neurons degrades previously acquired memories. J Neurosci 31(42):15113-27. [PubMed: 22016545]  [MGI Ref ID J:177636]

Chow A; Lucas D; Hidalgo A; Mendez-Ferrer S; Hashimoto D; Scheiermann C; Battista M; Leboeuf M; Prophete C; van Rooijen N; Tanaka M; Merad M; Frenette PS. 2011. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 208(2):261-71. [PubMed: 21282381]  [MGI Ref ID J:176846]

Cordoba-Chacon J; Gahete MD; Pokala NK; Geldermann D; Alba M; Salvatori R; Luque RM; Kineman RD. 2014. Long- but not short-term adult-onset, isolated GH deficiency in male mice leads to deterioration of beta-cell function, which cannot be accounted for by changes in beta-cell mass. Endocrinology 155(3):726-35. [PubMed: 24424062]  [MGI Ref ID J:208804]

De Filippo K; Dudeck A; Hasenberg M; Nye E; van Rooijen N; Hartmann K; Gunzer M; Roers A; Hogg N. 2013. Mast cell and macrophage chemokines CXCL1/CXCL2 control the early stage of neutrophil recruitment during tissue inflammation. Blood 121(24):4930-7. [PubMed: 23645836]  [MGI Ref ID J:198933]

Demehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676]  [MGI Ref ID J:146637]

Domingos AI; Sordillo A; Dietrich MO; Liu ZW; Tellez LA; Vaynshteyn J; Ferreira JG; Ekstrand MI; Horvath TL; de Araujo IE; Friedman JM. 2013. Hypothalamic melanin concentrating hormone neurons communicate the nutrient value of sugar. Elife 2:e01462. [PubMed: 24381247]  [MGI Ref ID J:206211]

Doucet YS; Woo SH; Ruiz ME; Owens DM. 2013. The touch dome defines an epidermal niche specialized for mechanosensory signaling. Cell Rep 3(6):1759-65. [PubMed: 23727240]  [MGI Ref ID J:199315]

Dudeck A; Dudeck J; Scholten J; Petzold A; Surianarayanan S; Kohler A; Peschke K; Vohringer D; Waskow C; Krieg T; Muller W; Waisman A; Hartmann K; Gunzer M; Roers A. 2011. Mast Cells Are Key Promoters of Contact Allergy that Mediate the Adjuvant Effects of Haptens. Immunity 34(6):973-84. [PubMed: 21703544]  [MGI Ref ID J:173991]

Durieux PF; Bearzatto B; Guiducci S; Buch T; Waisman A; Zoli M; Schiffmann SN; de Kerchove d'Exaerde A. 2009. D2R striatopallidal neurons inhibit both locomotor and drug reward processes. Nat Neurosci 12(4):393-5. [PubMed: 19270687]  [MGI Ref ID J:150475]

Durieux PF; Schiffmann SN; de Kerchove d'Exaerde A. 2012. Differential regulation of motor control and response to dopaminergic drugs by D1R and D2R neurons in distinct dorsal striatum subregions. EMBO J 31(3):640-53. [PubMed: 22068054]  [MGI Ref ID J:181813]

Ena SL; De Backer JF; Schiffmann SN; de Kerchove d'Exaerde A. 2013. FACS array profiling identifies Ecto-5' nucleotidase as a striatopallidal neuron-specific gene involved in striatal-dependent learning. J Neurosci 33(20):8794-809. [PubMed: 23678122]  [MGI Ref ID J:198421]

Fooksman DR; Nussenzweig MC; Dustin ML. 2014. Myeloid cells limit production of antibody-secreting cells after immunization in the lymph node. J Immunol 192(3):1004-12. [PubMed: 24376270]  [MGI Ref ID J:207304]

Fu Q; Gremeaux L; Luque RM; Liekens D; Chen J; Buch T; Waisman A; Kineman R; Vankelecom H. 2012. The adult pituitary shows stem/progenitor cell activation in response to injury and is capable of regeneration. Endocrinology 153(7):3224-35. [PubMed: 22518061]  [MGI Ref ID J:188660]

Gahete MD; Cordoba-Chacon J; Luque RM; Kineman RD. 2013. The rise in growth hormone during starvation does not serve to maintain glucose levels or lean mass but is required for appropriate adipose tissue response in female mice. Endocrinology 154(1):263-9. [PubMed: 23150490]  [MGI Ref ID J:193144]

Goren I; Allmann N; Yogev N; Schurmann C; Linke A; Holdener M; Waisman A; Pfeilschifter J; Frank S. 2009. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol 175(1):132-47. [PubMed: 19528348]  [MGI Ref ID J:150035]

Grgic I; Campanholle G; Bijol V; Wang C; Sabbisetti VS; Ichimura T; Humphreys BD; Bonventre JV. 2012. Targeted proximal tubule injury triggers interstitial fibrosis and glomerulosclerosis. Kidney Int 82(2):172-83. [PubMed: 22437410]  [MGI Ref ID J:198182]

Han JH; Kushner SA; Yiu AP; Hsiang HL; Buch T; Waisman A; Bontempi B; Neve RL; Frankland PW; Josselyn SA. 2009. Selective erasure of a fear memory. Science 323(5920):1492-6. [PubMed: 19286560]  [MGI Ref ID J:145905]

Han L; Ma C; Liu Q; Weng HJ; Cui Y; Tang Z; Kim Y; Nie H; Qu L; Patel KN; Li Z; McNeil B; He S; Guan Y; Xiao B; Lamotte RH; Dong X. 2013. A subpopulation of nociceptors specifically linked to itch. Nat Neurosci 16(2):174-82. [PubMed: 23263443]  [MGI Ref ID J:197482]

Hatori M; Le H; Vollmers C; Keding SR; Tanaka N; Schmedt C; Jegla T; Panda S. 2008. Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3(6):e2451. [PubMed: 18545654]  [MGI Ref ID J:137151]

Jang JY; Koh YJ; Lee SH; Lee J; Kim KH; Kim D; Koh GY; Yoo OJ. 2013. Conditional ablation of LYVE-1+ cells unveils defensive roles of lymphatic vessels in intestine and lymph nodes. Blood 122(13):2151-61. [PubMed: 23836558]  [MGI Ref ID J:202246]

Kometani K; Nakagawa R; Shinnakasu R; Kaji T; Rybouchkin A; Moriyama S; Furukawa K; Koseki H; Takemori T; Kurosaki T. 2013. Repression of the transcription factor Bach2 contributes to predisposition of IgG1 memory B cells toward plasma cell differentiation. Immunity 39(1):136-47. [PubMed: 23850379]  [MGI Ref ID J:202810]

Landsman L; Nijagal A; Whitchurch TJ; Vanderlaan RL; Zimmer WE; Mackenzie TC; Hebrok M. 2011. Pancreatic mesenchyme regulates epithelial organogenesis throughout development. PLoS Biol 9(9):e1001143. [PubMed: 21909240]  [MGI Ref ID J:182781]

Li L; Ginty DD. 2014. The structure and organization of lanceolate mechanosensory complexes at mouse hair follicles. Elife 3:e01901. [PubMed: 24569481]  [MGI Ref ID J:207967]

Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217]  [MGI Ref ID J:186240]

Lucas D; Scheiermann C; Chow A; Kunisaki Y; Bruns I; Barrick C; Tessarollo L; Frenette PS. 2013. Chemotherapy-induced bone marrow nerve injury impairs hematopoietic regeneration. Nat Med 19(6):695-703. [PubMed: 23644514]  [MGI Ref ID J:198559]

Lucas T; Waisman A; Ranjan R; Roes J; Krieg T; Muller W; Roers A; Eming SA. 2010. Differential roles of macrophages in diverse phases of skin repair. J Immunol 184(7):3964-77. [PubMed: 20176743]  [MGI Ref ID J:160100]

Luque RM; Lin Q; Cordoba-Chacon J; Subbaiah PV; Buch T; Waisman A; Vankelecom H; Kineman RD. 2011. Metabolic impact of adult-onset, isolated, growth hormone deficiency (AOiGHD) due to destruction of pituitary somatotropes. PLoS One 6(1):e15767. [PubMed: 21283519]  [MGI Ref ID J:169459]

Mayer C; Boehm U. 2011. Female reproductive maturation in the absence of kisspeptin/GPR54 signaling. Nat Neurosci 14(6):704-10. [PubMed: 21516099]  [MGI Ref ID J:173936]

Mederacke I; Hsu CC; Troeger JS; Huebener P; Mu X; Dapito DH; Pradere JP; Schwabe RF. 2013. Fate tracing reveals hepatic stellate cells as dominant contributors to liver fibrosis independent of its aetiology. Nat Commun 4:2823. [PubMed: 24264436]  [MGI Ref ID J:205330]

Mendez-Ferrer S; Michurina TV; Ferraro F; Mazloom AR; Macarthur BD; Lira SA; Scadden DT; Ma'ayan A; Enikolopov GN; Frenette PS. 2010. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 466(7308):829-34. [PubMed: 20703299]  [MGI Ref ID J:163310]

Nakanishi Y; Seno H; Fukuoka A; Ueo T; Yamaga Y; Maruno T; Nakanishi N; Kanda K; Komekado H; Kawada M; Isomura A; Kawada K; Sakai Y; Yanagita M; Kageyama R; Kawaguchi Y; Taketo MM; Yonehara S; Chiba T. 2013. Dclk1 distinguishes between tumor and normal stem cells in the intestine. Nat Genet 45(1):98-103. [PubMed: 23202126]  [MGI Ref ID J:193873]

Narni-Mancinelli E; Chaix J; Fenis A; Kerdiles YM; Yessaad N; Reynders A; Gregoire C; Luche H; Ugolini S; Tomasello E; Walzer T; Vivier E. 2011. Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor. Proc Natl Acad Sci U S A 108(45):18324-9. [PubMed: 22021440]  [MGI Ref ID J:180265]

Onder L; Narang P; Scandella E; Chai Q; Iolyeva M; Hoorweg K; Halin C; Richie E; Kaye P; Westermann J; Cupedo T; Coles M; Ludewig B. 2012. IL-7-producing stromal cells are critical for lymph node remodeling. Blood 120(24):4675-83. [PubMed: 22955921]  [MGI Ref ID J:192130]

Owens BM; Beattie L; Moore JW; Brown N; Mann JL; Dalton JE; Maroof A; Kaye PM. 2012. IL-10-producing Th1 cells and disease progression are regulated by distinct CD11c(+) cell populations during visceral leishmaniasis. PLoS Pathog 8(7):e1002827. [PubMed: 22911108]  [MGI Ref ID J:195372]

Park D; Spencer JA; Koh BI; Kobayashi T; Fujisaki J; Clemens TL; Lin CP; Kronenberg HM; Scadden DT. 2012. Endogenous bone marrow MSCs are dynamic, fate-restricted participants in bone maintenance and regeneration. Cell Stem Cell 10(3):259-72. [PubMed: 22385654]  [MGI Ref ID J:185671]

Parkhurst CN; Yang G; Ninan I; Savas JN; Yates JR 3rd; Lafaille JJ; Hempstead BL; Littman DR; Gan WB. 2013. Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor. Cell 155(7):1596-609. [PubMed: 24360280]  [MGI Ref ID J:205483]

Pierson W; Cauwe B; Policheni A; Schlenner SM; Franckaert D; Berges J; Humblet-Baron S; Schonefeldt S; Herold MJ; Hildeman D; Strasser A; Bouillet P; Lu LF; Matthys P; Freitas AA; Luther RJ; Weaver CT; Dooley J; Gray DH; Liston A. 2013. Antiapoptotic Mcl-1 is critical for the survival and niche-filling capacity of Foxp3(+) regulatory T cells. Nat Immunol 14(9):959-65. [PubMed: 23852275]  [MGI Ref ID J:208243]

Reber LL; Marichal T; Mukai K; Kita Y; Tokuoka SM; Roers A; Hartmann K; Karasuyama H; Nadeau KC; Tsai M; Galli SJ. 2013. Selective ablation of mast cells or basophils reduces peanut-induced anaphylaxis in mice. J Allergy Clin Immunol 132(4):881-8.e1-11. [PubMed: 23915716]  [MGI Ref ID J:205536]

Sale S; Lafkas D; Artavanis-Tsakonas S. 2013. Notch2 genetic fate mapping reveals two previously unrecognized mammary epithelial lineages. Nat Cell Biol 15(5):451-60. [PubMed: 23604318]  [MGI Ref ID J:198503]

Sharma MD; Hou DY; Baban B; Koni PA; He Y; Chandler PR; Blazar BR; Mellor AL; Munn DH. 2010. Reprogrammed foxp3(+) regulatory T cells provide essential help to support cross-presentation and CD8(+) T cell priming in naive mice. Immunity 33(6):942-54. [PubMed: 21145762]  [MGI Ref ID J:167291]

Shiota C; Prasadan K; Guo P; El-Gohary Y; Wiersch J; Xiao X; Esni F; Gittes GK. 2013. alpha-Cells are dispensable in postnatal morphogenesis and maturation of mouse pancreatic islets. Am J Physiol Endocrinol Metab 305(8):E1030-40. [PubMed: 23982158]  [MGI Ref ID J:203933]

Thirumangalathu S; Harlow DE; Driskell AL; Krimm RF; Barlow LA. 2009. Fate mapping of mammalian embryonic taste bud progenitors. Development 136(9):1519-28. [PubMed: 19363153]  [MGI Ref ID J:147959]

Uhlenhaut NH; Jakob S; Anlag K; Eisenberger T; Sekido R; Kress J; Treier AC; Klugmann C; Klasen C; Holter NI; Riethmacher D; Schutz G; Cooney AJ; Lovell-Badge R; Treier M. 2009. Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. Cell 139(6):1130-42. [PubMed: 20005806]  [MGI Ref ID J:157008]

Wang S; Ware SM. 2009. Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression. Genesis 47(2):132-6. [PubMed: 19165828]  [MGI Ref ID J:147303]

Wang X; Cho B; Suzuki K; Xu Y; Green JA; An J; Cyster JG. 2011. Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers. J Exp Med 208(12):2497-510. [PubMed: 22042977]  [MGI Ref ID J:178640]

Wenzel P; Knorr M; Kossmann S; Stratmann J; Hausding M; Schuhmacher S; Karbach SH; Schwenk M; Yogev N; Schulz E; Oelze M; Grabbe S; Jonuleit H; Becker C; Daiber A; Waisman A; Munzel T. 2011. Lysozyme M-positive monocytes mediate angiotensin II-induced arterial hypertension and vascular dysfunction. Circulation 124(12):1370-81. [PubMed: 21875910]  [MGI Ref ID J:189377]

Willenborg S; Eckes B; Brinckmann J; Krieg T; Waisman A; Hartmann K; Roers A; Eming SA. 2014. Genetic ablation of mast cells redefines the role of mast cells in skin wound healing and bleomycin-induced fibrosis. J Invest Dermatol 134(7):2005-15. [PubMed: 24406680]  [MGI Ref ID J:210854]

Wu Z; Xu Y; Zhu Y; Sutton AK; Zhao R; Lowell BB; Olson DP; Tong Q. 2012. An obligate role of oxytocin neurons in diet induced energy expenditure. PLoS One 7(9):e45167. [PubMed: 23028821]  [MGI Ref ID J:192007]

Xi D; Gandhi N; Lai M; Kublaoui BM. 2012. Ablation of Sim1 neurons causes obesity through hyperphagia and reduced energy expenditure. PLoS One 7(4):e36453. [PubMed: 22558467]  [MGI Ref ID J:210790]

Xi D; Roizen J; Lai M; Gandhi N; Kublaoui B. 2013. Paraventricular nucleus Sim1 neuron ablation mediated obesity is resistant to high fat diet. PLoS One 8(11):e81087. [PubMed: 24260538]  [MGI Ref ID J:209672]

Yi T; Wang X; Kelly LM; An J; Xu Y; Sailer AW; Gustafsson JA; Russell DW; Cyster JG. 2012. Oxysterol Gradient Generation by Lymphoid Stromal Cells Guides Activated B Cell Movement during Humoral Responses. Immunity 37(3):535-48. [PubMed: 22999953]  [MGI Ref ID J:188276]

Yogev N; Frommer F; Lukas D; Kautz-Neu K; Karram K; Ielo D; von Stebut E; Probst HC; van den Broek M; Riethmacher D; Birnberg T; Blank T; Reizis B; Korn T; Wiendl H; Jung S; Prinz M; Kurschus FC; Waisman A. 2012. Dendritic Cells Ameliorate Autoimmunity in the CNS by Controlling the Homeostasis of PD-1 Receptor(+) Regulatory T Cells. Immunity 37(2):264-75. [PubMed: 22902234]  [MGI Ref ID J:187367]

van Montfoort N; Mangsbo SM; Camps MG; van Maren WW; Verhaart IE; Waisman A; Drijfhout JW; Melief CJ; Verbeek JS; Ossendorp F. 2012. Circulating specific antibodies enhance systemic cross-priming by delivery of complexed antigen to dendritic cells in vivo. Eur J Immunol 42(3):598-606. [PubMed: 22488363]  [MGI Ref ID J:187782]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryMutant mice were bred to BALB/cByJ mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
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  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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