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| The Cre-inducible expression of DTR in these iDTR mutant mice render cells susceptible to ablation following Diphtheria toxin administration. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5F4pN1
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.
When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. 011069), this mutant mouse strain may be useful in studies of metabolic dysfunction.
Of note, iDTR mice are also available on a C57BL/6 background (as Stock No. 007900) and a NOD congenic background (as Stock No. 016603).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. These iDTR mice were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory (as Stock No. 007900). Upon arrival, some mice were additionally backcrossed to BALB/cByJ (Stock No. 001026) to generate this congenic strain (Stock No. 008040).
Control Information
| Control | ||
|---|---|---|
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(HBEGF)Awai allele
007900 C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J 016603 NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ View Strains carrying Gt(ROSA)26Sortm1(HBEGF)Awai (2 strains)
014176 C57BL/6-Tg(CLEC4C-HBEGF)956Cln/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Cd19tm1(cre)Cgn/? Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
involves: 129P2/OlaHsd * C57BL/6 (conditional)
- immune system phenotype
- abnormal lymph node cell ratio
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the lymph nodes (MGI Ref ID J:131076)
- abnormal lymphocyte morphology
- after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice (MGI Ref ID J:131076)
- abnormal splenic cell ratio
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen (MGI Ref ID J:131076)
- hematopoietic system phenotype
- abnormal bone marrow cell number
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed (MGI Ref ID J:131076)
- abnormal lymphocyte morphology
- after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice (MGI Ref ID J:131076)
- abnormal splenic cell ratio
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen (MGI Ref ID J:131076)
- cellular phenotype
- absent immature B cells
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed (MGI Ref ID J:131076)
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+ Tg(Gh1-cre)bKnmn/0
involves: C57BL/6 * FVB/N (conditional)
- homeostasis/metabolism phenotype
- abnormal circulating hormone level (MGI Ref ID J:169459)
- decreased circulating growth hormone level
- in diphtheria toxin-treated mice fed a high-fat or low-fat diet (MGI Ref ID J:169459)
- decreased circulating insulin level
- in diphtheria toxin-treated mice when a high-fat diet or a low-fat diet (MGI Ref ID J:169459)
- decreased circulating insulin-like growth factor I level
- in diphtheria toxin-treated mice fed a high-fat or low-fat diet (MGI Ref ID J:169459)
- increased circulating leptin level
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- abnormal energy expenditure (MGI Ref ID J:169459)
- decreased energy expenditure
- in diphtheria toxin-treated mice fed a low-fat during the nocturnal phase or when mice are fed a high-fat diet (MGI Ref ID J:169459)
- abnormal gas homeostasis (MGI Ref ID J:169459)
- abnormal glucose homeostasis (MGI Ref ID J:169459)
- decreased circulating glucose level
- decreased circulating insulin level
- in diphtheria toxin-treated mice when a high-fat diet or a low-fat diet (MGI Ref ID J:169459)
- impaired glucose tolerance
- in diphtheria toxin-treated mice fed a high-fat (MGI Ref ID J:169459)
- increased insulin sensitivity
- in diphtheria toxin-treated mice when fed standard chow, a high-fat diet, or a low-fat diet (MGI Ref ID J:169459)
- decreased liver triglyceride level
- in diphtheria toxin-treated mice fed a high-fat (MGI Ref ID J:169459)
- endocrine/exocrine gland phenotype
- decreased somatotroph cell number
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- small adenohypophysis
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- adipose tissue phenotype
- increased retroperitoneal fat pad weight
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- increased subcutaneous adipose tissue amount
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- increased total body fat amount
- in diphtheria toxin-treated mice fed standard chow or a high fat diet (MGI Ref ID J:169459)
- growth/size phenotype
- decreased body weight
- in diphtheria toxin-treated mice fed a high fat diet (MGI Ref ID J:169459)
- decreased lean body mass
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- liver/biliary system phenotype
- decreased liver triglyceride level
- in diphtheria toxin-treated mice fed a high-fat (MGI Ref ID J:169459)
- decreased liver weight
- in diphtheria toxin-treated mice fed standard chow or a high fat diet (MGI Ref ID J:169459)
- nervous system phenotype
- decreased somatotroph cell number
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- small adenohypophysis
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
- behavior/neurological phenotype
- decreased fluid intake
- in diphtheria toxin-treated mice fed a low-fat (MGI Ref ID J:169459)
- integument phenotype
- increased subcutaneous adipose tissue amount
- in diphtheria toxin-treated mice (MGI Ref ID J:169459)
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai Tg(Gdf9-cre)5092Coo/0
involves: C57BL/6 (conditional)
- reproductive system phenotype
- abnormal ovary morphology (MGI Ref ID J:157008)
- abnormal ovarian follicle morphology
- with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis (MGI Ref ID J:157008)
- abnormal granulosa cell morphology
- cells have undergone apoptosis in preantral follicles (MGI Ref ID J:157008)
- oocyte degeneration
- oocytes have undergone apoptosis in preantral follicles (MGI Ref ID J:157008)
- endocrine/exocrine gland phenotype
- abnormal ovary morphology (MGI Ref ID J:157008)
- abnormal ovarian follicle morphology
- with diptheria toxin administration to 8-week-old pregnant females, oocytes and granulosa cells in preantral oocytes show apoptosis 9 days later, in contrast to treated controls where atretic follicles but not preantral follicles show apoptosis (MGI Ref ID J:157008)
- abnormal granulosa cell morphology
- cells have undergone apoptosis in preantral follicles (MGI Ref ID J:157008)
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Research Tools
Cardiovascular Research
Cre-lox System
Cell Biology Research
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Diabetes and Obesity Research
loxP
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(HBEGF)Awai | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ari Waisman | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26iDTR; ROSA26-DTR; Rosa26iDTR; RosaiDTR; iDTR; | ||
| Mutation Made By | Ari Waisman, Johannes Gutenberg University of Mainz | ||
| Strain of Origin | B6.Cg-Thy1 | ||
| ES Cell Line Name | Bruce 4 | ||
| ES Cell Line Strain | B6.Cg-Thy1 | ||
| Expressed Gene | HBEGF, heparin-binding EGF-like growth factor, chimpanzee | ||
| General Note | Phenotypic Similarity to Human Syndrome: Isolated Growth Hormone Deficiency, Adult Onset (J:169459) | ||
| Molecular Note | A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. [MGI Ref ID J:131076] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(HBEGF)Awai, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920] [MGI Ref ID J:131076]
Additional References
Gt(ROSA)26Sortm1(HBEGF)Awai relatedArruda-Carvalho M; Sakaguchi M; Akers KG; Josselyn SA; Frankland PW. 2011. Posttraining ablation of adult-generated neurons degrades previously acquired memories. J Neurosci 31(42):15113-27. [PubMed: 22016545] [MGI Ref ID J:177636]
Chow A; Lucas D; Hidalgo A; Mendez-Ferrer S; Hashimoto D; Scheiermann C; Battista M; Leboeuf M; Prophete C; van Rooijen N; Tanaka M; Merad M; Frenette PS. 2011. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 208(2):261-71. [PubMed: 21282381] [MGI Ref ID J:176846]
Demehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676] [MGI Ref ID J:146637]
Dudeck A; Dudeck J; Scholten J; Petzold A; Surianarayanan S; Kohler A; Peschke K; Vohringer D; Waskow C; Krieg T; Muller W; Waisman A; Hartmann K; Gunzer M; Roers A. 2011. Mast Cells Are Key Promoters of Contact Allergy that Mediate the Adjuvant Effects of Haptens. Immunity 34(6):973-84. [PubMed: 21703544] [MGI Ref ID J:173991]
Durieux PF; Bearzatto B; Guiducci S; Buch T; Waisman A; Zoli M; Schiffmann SN; de Kerchove d'Exaerde A. 2009. D2R striatopallidal neurons inhibit both locomotor and drug reward processes. Nat Neurosci 12(4):393-5. [PubMed: 19270687] [MGI Ref ID J:150475]
Durieux PF; Schiffmann SN; de Kerchove d'Exaerde A. 2012. Differential regulation of motor control and response to dopaminergic drugs by D1R and D2R neurons in distinct dorsal striatum subregions. EMBO J 31(3):640-53. [PubMed: 22068054] [MGI Ref ID J:181813]
Fu Q; Gremeaux L; Luque RM; Liekens D; Chen J; Buch T; Waisman A; Kineman R; Vankelecom H. 2012. The adult pituitary shows stem/progenitor cell activation in response to injury and is capable of regeneration. Endocrinology 153(7):3224-35. [PubMed: 22518061] [MGI Ref ID J:188660]
Gahete MD; Cordoba-Chacon J; Luque RM; Kineman RD. 2013. The rise in growth hormone during starvation does not serve to maintain glucose levels or lean mass but is required for appropriate adipose tissue response in female mice. Endocrinology 154(1):263-9. [PubMed: 23150490] [MGI Ref ID J:193144]
Goren I; Allmann N; Yogev N; Schurmann C; Linke A; Holdener M; Waisman A; Pfeilschifter J; Frank S. 2009. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol 175(1):132-47. [PubMed: 19528348] [MGI Ref ID J:150035]
Han JH; Kushner SA; Yiu AP; Hsiang HL; Buch T; Waisman A; Bontempi B; Neve RL; Frankland PW; Josselyn SA. 2009. Selective erasure of a fear memory. Science 323(5920):1492-6. [PubMed: 19286560] [MGI Ref ID J:145905]
Hatori M; Le H; Vollmers C; Keding SR; Tanaka N; Schmedt C; Jegla T; Panda S. 2008. Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3(6):e2451. [PubMed: 18545654] [MGI Ref ID J:137151]
Landsman L; Nijagal A; Whitchurch TJ; Vanderlaan RL; Zimmer WE; Mackenzie TC; Hebrok M. 2011. Pancreatic mesenchyme regulates epithelial organogenesis throughout development. PLoS Biol 9(9):e1001143. [PubMed: 21909240] [MGI Ref ID J:182781]
Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217] [MGI Ref ID J:186240]
Lucas T; Waisman A; Ranjan R; Roes J; Krieg T; Muller W; Roers A; Eming SA. 2010. Differential roles of macrophages in diverse phases of skin repair. J Immunol 184(7):3964-77. [PubMed: 20176743] [MGI Ref ID J:160100]
Luque RM; Lin Q; Cordoba-Chacon J; Subbaiah PV; Buch T; Waisman A; Vankelecom H; Kineman RD. 2011. Metabolic impact of adult-onset, isolated, growth hormone deficiency (AOiGHD) due to destruction of pituitary somatotropes. PLoS One 6(1):e15767. [PubMed: 21283519] [MGI Ref ID J:169459]
Mayer C; Boehm U. 2011. Female reproductive maturation in the absence of kisspeptin/GPR54 signaling. Nat Neurosci 14(6):704-10. [PubMed: 21516099] [MGI Ref ID J:173936]
Mendez-Ferrer S; Michurina TV; Ferraro F; Mazloom AR; Macarthur BD; Lira SA; Scadden DT; Ma'ayan A; Enikolopov GN; Frenette PS. 2010. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 466(7308):829-34. [PubMed: 20703299] [MGI Ref ID J:163310]
Nakanishi Y; Seno H; Fukuoka A; Ueo T; Yamaga Y; Maruno T; Nakanishi N; Kanda K; Komekado H; Kawada M; Isomura A; Kawada K; Sakai Y; Yanagita M; Kageyama R; Kawaguchi Y; Taketo MM; Yonehara S; Chiba T. 2013. Dclk1 distinguishes between tumor and normal stem cells in the intestine. Nat Genet 45(1):98-103. [PubMed: 23202126] [MGI Ref ID J:193873]
Narni-Mancinelli E; Chaix J; Fenis A; Kerdiles YM; Yessaad N; Reynders A; Gregoire C; Luche H; Ugolini S; Tomasello E; Walzer T; Vivier E. 2011. Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor. Proc Natl Acad Sci U S A 108(45):18324-9. [PubMed: 22021440] [MGI Ref ID J:180265]
Onder L; Narang P; Scandella E; Chai Q; Iolyeva M; Hoorweg K; Halin C; Richie E; Kaye P; Westermann J; Cupedo T; Coles M; Ludewig B. 2012. IL-7-producing stromal cells are critical for lymph node remodeling. Blood 120(24):4675-83. [PubMed: 22955921] [MGI Ref ID J:192130]
Owens BM; Beattie L; Moore JW; Brown N; Mann JL; Dalton JE; Maroof A; Kaye PM. 2012. IL-10-producing Th1 cells and disease progression are regulated by distinct CD11c(+) cell populations during visceral leishmaniasis. PLoS Pathog 8(7):e1002827. [PubMed: 22911108] [MGI Ref ID J:195372]
Park D; Spencer JA; Koh BI; Kobayashi T; Fujisaki J; Clemens TL; Lin CP; Kronenberg HM; Scadden DT. 2012. Endogenous bone marrow MSCs are dynamic, fate-restricted participants in bone maintenance and regeneration. Cell Stem Cell 10(3):259-72. [PubMed: 22385654] [MGI Ref ID J:185671]
Sharma MD; Hou DY; Baban B; Koni PA; He Y; Chandler PR; Blazar BR; Mellor AL; Munn DH. 2010. Reprogrammed foxp3(+) regulatory T cells provide essential help to support cross-presentation and CD8(+) T cell priming in naive mice. Immunity 33(6):942-54. [PubMed: 21145762] [MGI Ref ID J:167291]
Thirumangalathu S; Harlow DE; Driskell AL; Krimm RF; Barlow LA. 2009. Fate mapping of mammalian embryonic taste bud progenitors. Development 136(9):1519-28. [PubMed: 19363153] [MGI Ref ID J:147959]
Uhlenhaut NH; Jakob S; Anlag K; Eisenberger T; Sekido R; Kress J; Treier AC; Klugmann C; Klasen C; Holter NI; Riethmacher D; Schutz G; Cooney AJ; Lovell-Badge R; Treier M. 2009. Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. Cell 139(6):1130-42. [PubMed: 20005806] [MGI Ref ID J:157008]
Wang S; Ware SM. 2009. Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression. Genesis 47(2):132-6. [PubMed: 19165828] [MGI Ref ID J:147303]
Wang X; Cho B; Suzuki K; Xu Y; Green JA; An J; Cyster JG. 2011. Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers. J Exp Med 208(12):2497-510. [PubMed: 22042977] [MGI Ref ID J:178640]
Wenzel P; Knorr M; Kossmann S; Stratmann J; Hausding M; Schuhmacher S; Karbach SH; Schwenk M; Yogev N; Schulz E; Oelze M; Grabbe S; Jonuleit H; Becker C; Daiber A; Waisman A; Munzel T. 2011. Lysozyme M-positive monocytes mediate angiotensin II-induced arterial hypertension and vascular dysfunction. Circulation 124(12):1370-81. [PubMed: 21875910] [MGI Ref ID J:189377]
Yi T; Wang X; Kelly LM; An J; Xu Y; Sailer AW; Gustafsson JA; Russell DW; Cyster JG. 2012. Oxysterol Gradient Generation by Lymphoid Stromal Cells Guides Activated B Cell Movement during Humoral Responses. Immunity 37(3):535-48. [PubMed: 22999953] [MGI Ref ID J:188276]
Yogev N; Frommer F; Lukas D; Kautz-Neu K; Karram K; Ielo D; von Stebut E; Probst HC; van den Broek M; Riethmacher D; Birnberg T; Blank T; Reizis B; Korn T; Wiendl H; Jung S; Prinz M; Kurschus FC; Waisman A. 2012. Dendritic Cells Ameliorate Autoimmunity in the CNS by Controlling the Homeostasis of PD-1 Receptor(+) Regulatory T Cells. Immunity 37(2):264-75. [PubMed: 22902234] [MGI Ref ID J:187367]
van Montfoort N; Mangsbo SM; Camps MG; van Maren WW; Verhaart IE; Waisman A; Drijfhout JW; Melief CJ; Verbeek JS; Ossendorp F. 2012. Circulating specific antibodies enhance systemic cross-priming by delivery of complexed antigen to dendritic cells in vivo. Eur J Immunol 42(3):598-606. [PubMed: 22488363] [MGI Ref ID J:187782]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Mutant mice were bred to BALB/cByJ mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.