Strain Name:

CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J

Stock Number:

008040

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
The Cre-inducible expression of DTR in these iDTR mutant mice render cells susceptible to ablation following Diphtheria toxin administration.

Description

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   09-JUN-09
Specieslaboratory mouse
GenerationN5 (26-OCT-09)
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. These iDTR mice were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory (as Stock No. 007900). Upon arrival, some mice were additionally backcrossed to BALB/cByJ (Stock No. 001026) to generate this congenic strain (Stock No. 008040).

Control Information

  Control
   Wild-type from the colony
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Gt(ROSA)26Sortm1(HBEGF)Awai allele
007900   C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
View Strains carrying   Gt(ROSA)26Sortm1(HBEGF)Awai     (1 strain)

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003310   129S-Gt(ROSA)26Sortm1Sor/J
009043   129S-Gt(ROSA)26Sortm2Tyj/J
003946   129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
010633   B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
008463   B6.129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
009669   B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J
008513   B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J
008600   B6.129P2-Gt(ROSA)26Sortm1(tTA)Roos/J
009086   B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
009044   B6.129S4-Gt(ROSA)26Sortm2Tyj/J
007743   B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007920   B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
007903   B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
007906   B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
008883   B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
008516   B6;129-Gt(ROSA)26Sortm1Joe/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
008889   B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008886   B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ
010523   B6;129P2-Gt(ROSA)26Sortm1(CAG-ALPP)Fawa/J
002073   B6;129S-Gt(ROSA)26Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
007908   B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007905   B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
002955   C.129S7-Gt(ROSA)26Sor/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIRN17-92,-EGFP)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
009427   FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
010920   FVB;129P2-Gt(ROSA)26Sortm1(birA)Mejr/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
008159   STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (63 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Cd19tm1(cre)Cgn/? Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai

        involves: 129P2/OlaHsd * C57BL/6   (conditional)
  • immune system phenotype
  • abnormal lymph node cell ratio (MGI Ref ID J:131076)
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the lymph nodes
  • abnormal lymphocyte morphology (MGI Ref ID J:131076)
    • after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice
    • abnormal B cell morphology (MGI Ref ID J:131076)
      • CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
    • abnormal lymphocyte cell number (MGI Ref ID J:131076)
      • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
  • abnormal splenic cell ratio (MGI Ref ID J:131076)
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen
  • hematopoietic system phenotype
  • abnormal bone marrow cell number (MGI Ref ID J:131076)
    • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
  • abnormal lymphocyte morphology (MGI Ref ID J:131076)
    • after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice
    • abnormal B cell morphology (MGI Ref ID J:131076)
      • CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
    • abnormal lymphocyte cell number (MGI Ref ID J:131076)
      • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent immature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
      • absent mature B cells (MGI Ref ID J:131076)
        • in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
  • abnormal splenic cell ratio (MGI Ref ID J:131076)
    • with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cardiovascular Research
      Cre-lox System
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Gt(ROSA)26Sortm1(HBEGF)Awai
Allele Name targeted mutation 1, Ari Waisman
Allele Type Targeted (knock-in)
Common Name(s) R26iDTR; ROSA26-DTR; iDTR;
Mutation Made By Ari Waisman,   Johannes Gutenberg University of Mainz
Strain of OriginC57BL/6
ES Cell Line NameBruce 4
ES Cell Line StrainC57BL/6
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;
Molecular Note A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. [MGI Ref ID J:131076] [MGI Ref ID J:64292]

Genotyping

Genotyping Information

Genotyping Protocols

Gt(ROSA)26Sortm1(HBEGF)Awai, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920]  [MGI Ref ID J:131076]

Additional References

Gt(ROSA)26Sortm1(HBEGF)Awai related

Demehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676]  [MGI Ref ID J:146637]

Durieux PF; Bearzatto B; Guiducci S; Buch T; Waisman A; Zoli M; Schiffmann SN; de Kerchove d'Exaerde A. 2009. D2R striatopallidal neurons inhibit both locomotor and drug reward processes. Nat Neurosci 12(4):393-5. [PubMed: 19270687]  [MGI Ref ID J:150475]

Goren I; Allmann N; Yogev N; Schurmann C; Linke A; Holdener M; Waisman A; Pfeilschifter J; Frank S. 2009. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol 175(1):132-47. [PubMed: 19528348]  [MGI Ref ID J:150035]

Han JH; Kushner SA; Yiu AP; Hsiang HL; Buch T; Waisman A; Bontempi B; Neve RL; Frankland PW; Josselyn SA. 2009. Selective erasure of a fear memory. Science 323(5920):1492-6. [PubMed: 19286560]  [MGI Ref ID J:145905]

Hatori M; Le H; Vollmers C; Keding SR; Tanaka N; Schmedt C; Jegla T; Panda S. 2008. Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3(6):e2451. [PubMed: 18545654]  [MGI Ref ID J:137151]

Heusner CL; Beutler LR; Houser CR; Palmiter RD. 2008. Deletion of GAD67 in dopamine receptor-1 expressing cells causes specific motor deficits. Genesis 46(7):357-67. [PubMed: 18615733]  [MGI Ref ID J:138586]

Li M; Li C; Liu YH; Xing Y; Hu L; Borok Z; Kwong KY; Minoo P. 2008. Mesodermal Deletion of Transforming Growth Factor-{beta} Receptor II Disrupts Lung Epithelial Morphogenesis: cross-talk between TGF-{beta} and Sonic hedgehog pathways. J Biol Chem 283(52):36257-64. [PubMed: 18990706]  [MGI Ref ID J:143910]

Soriano P. 1999. Generalized lacZ expression with the ROSA26 Cre reporter strain [letter] Nat Genet 21(1):70-1. [PubMed: 9916792]  [MGI Ref ID J:64292]

Thirumangalathu S; Harlow DE; Driskell AL; Krimm RF; Barlow LA. 2009. Fate mapping of mammalian embryonic taste bud progenitors. Development 136(9):1519-28. [PubMed: 19363153]  [MGI Ref ID J:147959]

Wang S; Ware SM. 2009. Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression. Genesis 47(2):132-6. [PubMed: 19165828]  [MGI Ref ID J:147303]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryMutant mice were bred to BALB/cByJ mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   09-JUN-09
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $243.50Female or MaleHeterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Pairs /Price (US dollars $)Pair Genotype
$487.00Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai x Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $316.60Female or MaleHeterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Pairs /Price (US dollars $)Pair Genotype
$633.10Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai x Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   Wild-type from the colony
   001026 BALB/cByJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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