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| The Cre-inducible expression of DTR in these iDTR mutant mice render cells susceptible to ablation following Diphtheria toxin administration. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 09-JUN-09 Species laboratory mouse Generation N5 (26-OCT-09) Donating Investigator IMR Colony, The Jackson Laboratory Description
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. These iDTR mice were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory (as Stock No. 007900). Upon arrival, some mice were additionally backcrossed to BALB/cByJ (Stock No. 001026) to generate this congenic strain (Stock No. 008040).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(HBEGF)Awai allele
007900 C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J View Strains carrying Gt(ROSA)26Sortm1(HBEGF)Awai (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Cd19tm1(cre)Cgn/? Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
involves: 129P2/OlaHsd * C57BL/6 (conditional)
- immune system phenotype
- abnormal lymph node cell ratio (MGI Ref ID J:131076)
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the lymph nodes
- abnormal lymphocyte morphology (MGI Ref ID J:131076)
- after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice
- abnormal B cell morphology (MGI Ref ID J:131076)
- CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days
- absent immature B cells (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- absent mature B cells (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- abnormal lymphocyte cell number (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- abnormal splenic cell ratio (MGI Ref ID J:131076)
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen
- hematopoietic system phenotype
- abnormal bone marrow cell number (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- abnormal lymphocyte morphology (MGI Ref ID J:131076)
- after 3 or 7 days of diptheria toxin treatment, splenic B to T lymphocyte ratio decreases from 2.1 in controls to 0.6 and 0.3 respectively in double transgenic mice
- abnormal B cell morphology (MGI Ref ID J:131076)
- CD19-expressing B cells are absent after intraperitoneal injection of diptheria toxin for 7 days
- absent immature B cells (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- absent mature B cells (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- abnormal lymphocyte cell number (MGI Ref ID J:131076)
- in bone marrow, drastic reductions in numbers of immature and mature B cells is observed
- abnormal splenic cell ratio (MGI Ref ID J:131076)
- with diptheria toxin treatment 3 times daily for 7 days, B cells are virtually absent from the spleen
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Research Tools
Cardiovascular Research
Cre-lox System
Cell Biology Research
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Diabetes and Obesity Research
loxP
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(HBEGF)Awai | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ari Waisman | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26iDTR; ROSA26-DTR; iDTR; | ||
| Mutation Made By | Ari Waisman, Johannes Gutenberg University of Mainz | ||
| Strain of Origin | C57BL/6 | ||
| ES Cell Line Name | Bruce 4 | ||
| ES Cell Line Strain | C57BL/6 | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. [MGI Ref ID J:131076] [MGI Ref ID J:64292] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(HBEGF)Awai, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920] [MGI Ref ID J:131076]
Additional References
Gt(ROSA)26Sortm1(HBEGF)Awai relatedDemehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676] [MGI Ref ID J:146637]
Durieux PF; Bearzatto B; Guiducci S; Buch T; Waisman A; Zoli M; Schiffmann SN; de Kerchove d'Exaerde A. 2009. D2R striatopallidal neurons inhibit both locomotor and drug reward processes. Nat Neurosci 12(4):393-5. [PubMed: 19270687] [MGI Ref ID J:150475]
Goren I; Allmann N; Yogev N; Schurmann C; Linke A; Holdener M; Waisman A; Pfeilschifter J; Frank S. 2009. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol 175(1):132-47. [PubMed: 19528348] [MGI Ref ID J:150035]
Han JH; Kushner SA; Yiu AP; Hsiang HL; Buch T; Waisman A; Bontempi B; Neve RL; Frankland PW; Josselyn SA. 2009. Selective erasure of a fear memory. Science 323(5920):1492-6. [PubMed: 19286560] [MGI Ref ID J:145905]
Hatori M; Le H; Vollmers C; Keding SR; Tanaka N; Schmedt C; Jegla T; Panda S. 2008. Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3(6):e2451. [PubMed: 18545654] [MGI Ref ID J:137151]
Heusner CL; Beutler LR; Houser CR; Palmiter RD. 2008. Deletion of GAD67 in dopamine receptor-1 expressing cells causes specific motor deficits. Genesis 46(7):357-67. [PubMed: 18615733] [MGI Ref ID J:138586]
Li M; Li C; Liu YH; Xing Y; Hu L; Borok Z; Kwong KY; Minoo P. 2008. Mesodermal Deletion of Transforming Growth Factor-{beta} Receptor II Disrupts Lung Epithelial Morphogenesis: cross-talk between TGF-{beta} and Sonic hedgehog pathways. J Biol Chem 283(52):36257-64. [PubMed: 18990706] [MGI Ref ID J:143910]
Soriano P. 1999. Generalized lacZ expression with the ROSA26 Cre reporter strain [letter] Nat Genet 21(1):70-1. [PubMed: 9916792] [MGI Ref ID J:64292]
Thirumangalathu S; Harlow DE; Driskell AL; Krimm RF; Barlow LA. 2009. Fate mapping of mammalian embryonic taste bud progenitors. Development 136(9):1519-28. [PubMed: 19363153] [MGI Ref ID J:147959]
Wang S; Ware SM. 2009. Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression. Genesis 47(2):132-6. [PubMed: 19165828] [MGI Ref ID J:147303]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to BALB/cByJ mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together. Mating System Heterozygote x Heterozygote (Female x Male) 09-JUN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai x Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai x Heterozygous for Gt(ROSA)26Sortm1(HBEGF)Awai
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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