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| These SirT1co/co mice may be useful in generating conditional mutations for studying the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, breast cancer, apoptosis, and metabolic diseases. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Yansong Gu, University of Washington School of Med. Description
Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile. A loxP-flanked neomycin cassette just upstream of exon 4 and a third loxP site downstream of exon 4 were inserted to create this targeted mutant Sirt1 allele. The floxed mutation does not affect SIRT1 protein expression in MEFs or mammary gland tissue in homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding an evolutionarily conserved Sir2 motif) deleted in cre-expressing tissue(s); (the donating investigator reports only one recombination event: complete removal of the neomycin cassette and exon 4, leaving a single loxp). These SirT1co/co mice may be useful in generating conditional mutants for studying transcriptional regulation and the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, mammary cancer, apoptosis, and metabolic diseases.Development
A targeting vector was designed to insert a loxP-flanked, reverse-oriented neomycin cassette just upstream of exon 4 and a third loxP site downstream of exon 4 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the chimeras were bred with C57BL/6 mice. The resulting heterozygotes were interbred to generate homozygous SirT1co/co mice on this mixed B6;129 genetic background. Homozygotes were interbred for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
Sirt1tm1Ygu/Sirt1tm1Ygu
involves: 129/Sv * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:121948)
This mouse can be used to support research in many areas including:
Cancer Research
Growth Factors/Receptors/Cytokines
Other
Cell Biology Research
Transcriptional Regulation
Diabetes and Obesity Research
Insulin Receptors and Growth Factors
Endocrine Deficiency Research
Mammary Gland Defects
Research Tools
Apoptosis Research
Cancer Research
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Diabetes and Obesity Research (loxP)
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Metabolism Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Sirt1tm1Ygu | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Yansong Gu | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | SirT1co; | ||
| Mutation Made By | Yansong Gu, University of Washington School of Med. | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Sirt1, sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | AA673258; SIR2L1; Sir2; Sir2alpha; expressed sequence AA673258; | ||
| Molecular Note | A floxed neomycin cassette was inserted just upstream of exon 4 and a third loxP site was inserted just downstream. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. When bred tomice that express Cre recombinase, the resulting offspring will have exon 4 (encoding a conserved Sir2 motif) deleted in the cre-expressing tissue(s). [MGI Ref ID J:121948] [MGI Ref ID J:85542] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Sirt1tm1Ygu, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Li H; Rajendran GK; Liu N; Ware C; Rubin BP; Gu Y. 2007. SirT1 modulates the estrogen-insulin-like growth factor-1 signaling for postnatal development of mammary gland in mice. Breast Cancer Res 9(1):R1. [PubMed: 17201918] [MGI Ref ID J:121948]
Additional References
Sirt1tm1Ygu relatedChen D; Bruno J; Easlon E; Lin SJ; Cheng HL; Alt FW; Guarente L. 2008. Tissue-specific regulation of SIRT1 by calorie restriction. Genes Dev 22(13):1753-7. [PubMed: 18550784] [MGI Ref ID J:137371]
Cheng HL; Mostoslavsky R; Saito S; Manis JP; Gu Y; Patel P; Bronson R; Appella E; Alt FW; Chua KF. 2003. Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice. Proc Natl Acad Sci U S A 100(19):10794-9. [PubMed: 12960381] [MGI Ref ID J:85542]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Sirt1tm1Ygu *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Sirt1tm1Ygu x Homozygous for Sirt1tm1Ygu
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Sirt1tm1Ygu *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Sirt1tm1Ygu x Homozygous for Sirt1tm1Ygu
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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