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Strain Name:

B6;129-Sirt1tm1Ygu/J

Stock Number:

008041

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   Sirt1;   Sirt1tm1Ygu;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHeterozygote x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Yansong Gu,   University of Washington School of Med.

Strain Description
Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile. A loxP-flanked neomycin cassette just upstream of exon 4 and a third loxP site downstream of exon 4 were inserted to create this targeted mutant Sirt1 allele. The floxed mutation does not affect SIRT1 protein expression in MEFs or mammary gland tissue in homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding an evolutionarily conserved Sir2 motif) deleted in cre-expressing tissue(s); (the donating investigator reports only one recombination event: complete removal of the neomycin cassette and exon 4, leaving a single loxp). These SirT1co/co mice may be useful in generating conditional mutants for studying transcriptional regulation and the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, mammary cancer, apoptosis, and metabolic diseases.

Strain Development
A targeting vector was designed to insert a loxP-flanked, reverse-oriented neomycin cassette just upstream of exon 4 and a third loxP site downstream of exon 4 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the chimeras were bred with C57BL/6 mice. The resulting heterozygotes were interbred to generate homozygous SirT1co/co mice on this mixed B6;129 genetic background. Homozygotes were interbred for many generations prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype

Sirt1tm1Ygu/Sirt1tm1Ygu

        involves: 129/Sv * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:121948)

Gene & Allele Details

Allele Symbol Sirt1tm1Ygu
Allele Name targeted mutation 1, Yansong Gu
Common Name(s) SirT1co;
Mutation Made By Yansong Gu,   University of Washington School of Med.
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Sirt1, sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae)
Chromosome 10
Gene Common Name(s) AA673258; SIR2L1; Sir2; Sir2alpha; expressed sequence AA673258;
Molecular Note A floxed neomycin cassette was inserted just upstream of exon 4 and a third loxP site was inserted just downstream. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. When bred tomice that express Cre recombinase, the resulting offspring will have exon 4 (encoding a conserved Sir2 motif) deleted in the cre-expressing tissue(s). [MGI Ref ID J:121948] [MGI Ref ID J:85542]

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Sirt1tm1Ygu

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Diet Information LabDiet® 5K52/5K67

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines
Other

Cell Biology Research
Transcriptional Regulation

Diabetes and Obesity Research
Insulin Receptors and Growth Factors

Endocrine Deficiency Research
Mammary Gland Defects

Research Tools
Apoptosis Research
Cancer Research
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Diabetes and Obesity Research (loxP)
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Metabolism Research

References

Selected Reference(s)

Li H; Rajendran GK; Liu N; Ware C; Rubin BP; Gu Y. 2007. SirT1 modulates the estrogen-insulin-like growth factor-1 signaling for postnatal development of mammary gland in mice. Breast Cancer Res 9(1):R1. [PubMed: 17201918]  [MGI Ref ID J:121948]

Additional References

Price and Supply Information

Strain Name: B6;129-Sirt1tm1Ygu/J
Stock Number: 008041

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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