Strain Name: |
B6;129P2-Adam2tm1Dgm/J |
|---|---|
Stock Number: |
008042 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Adam2; Adam2tm1Dgm; |
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Paul Primakoff, University of California Davis Generation F?+ (23-JUN-08) Strain Description
Mice homozygous for this fertilin b (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin b proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin b (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).Strain Development
A targeting vector was designed to replace exon 14 of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd-derived BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6N to produce heterozygous mutant mice. The donating investigators maintained these mice by breeding homozygous females to heterozygous males for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Adam2tm1Dgm | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Diana G Myles | ||
| Common Name(s) | fertilin beta<->; | ||
| Mutation Made By | Diana Myles, University of California Davis | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Adam2, a disintegrin and metallopeptidase domain 2 | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | AI323749; CRYN1; CRYN2; FTNB; Ftnb; PH-30; PH-30b; PH30; Ph30-beta; expressed sequence AI323749; fertilin beta; | ||
| Molecular Note | A neomycin resistance cassette replaced a genomic fragment containing exon 14, encoding the predicted disintegrin domain active site. Western blot analysis did not detect the encoded protein in spermatogenic cells and sperm derived from homozygous mice. [MGI Ref ID J:50040] | ||
| Control | ||
|---|---|---|
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Adam2tm1Dgm
| Breeding & Husbandry | When maintaining a live colony, homozygous females are bred with heterozygous males. Homozygous males have severe sperm defects rendering them infertile. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Reproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)
Research Tools
Reproductive Biology Research (male germ cells)
Selected Reference(s)
Additional ReferencesCho C; Bunch DO; Faure JE; Goulding EH; Eddy EM; Primakoff P; Myles DG. 1998. Fertilization defects in sperm from mice lacking fertilin beta. Science 281(5384):1857-9. [PubMed: 9743500] [MGI Ref ID J:50040]
Nishimura H; Myles DG; Primakoff P. 2007. Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm. J Biol Chem 282(24):17900-7. [PubMed: 17439939] [MGI Ref ID J:123000]
| Strain Name: | B6;129P2-Adam2tm1Dgm/J |
| Stock Number: | 008042 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 29-SEP-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form