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Genes & Alleles

Adam2;   Adam2tm1Dgm;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Paul Primakoff,   University of California Davis Generation F?+ (23-JUN-08)

Strain Description
Mice homozygous for this fertilin b (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin b proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin b (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).

Strain Development
A targeting vector was designed to replace exon 14 of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd-derived BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6N to produce heterozygous mutant mice. The donating investigators maintained these mice by breeding homozygous females to heterozygous males for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Mammalian Phenotype Terms assigned by genotype Adam2tm1Dgm/Adam2tm1Dgm         involves: 129P2/OlaHsd * C57BL/6N reproductive system phenotype abnormal fertilization (MGI Ref ID J:50040) the ability of sperm from mutant mice to bind to and fuse with eggs is reduced once fused egg activation proceeds normally asthenozoospermia (MGI Ref ID J:50040) migration of sperm from the uterus into the oviduct is impaired sperm count and motility are otherwise normal reduced male fertility (MGI Ref ID J:50040) fertility rate is 2% compared to wild type

Gene & Allele Details

Allele Symbol		Adam2tm1Dgm
Allele Name		targeted mutation 1, Diana G Myles
Common Name(s)		fertilin beta<->;
Mutation Made By		Diana Myles,   University of California Davis
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14TG2a
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Adam2, a disintegrin and metallopeptidase domain 2
Chromosome		14
Gene Common Name(s)		AI323749; CRYN1; CRYN2; FTNB; Ftnb; PH-30; PH-30b; PH30; Ph30-beta; expressed sequence AI323749; fertilin beta;
Molecular Note		A neomycin resistance cassette replaced a genomic fragment containing exon 14, encoding the predicted disintegrin domain active site. Western blot analysis did not detect the encoded protein in spermatogenic cells and sperm derived from homozygous mice. [MGI Ref ID J:50040]

Control Information

	Control
	100492 B6129PF1/J	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Adam2^tm1Dgm

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous females are bred with heterozygous males. Homozygous males have severe sperm defects rendering them infertile.
Diet Information	LabDiet® 5K52/5K67

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)

Research Tools
Reproductive Biology Research (male germ cells)

References

Selected Reference(s)

Cho C; Bunch DO; Faure JE; Goulding EH; Eddy EM; Primakoff P; Myles DG. 1998. Fertilization defects in sperm from mice lacking fertilin beta. Science 281(5384):1857-9. [PubMed: 9743500]  [MGI Ref ID J:50040]

Nishimura H; Myles DG; Primakoff P. 2007. Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm. J Biol Chem 282(24):17900-7. [PubMed: 17439939]  [MGI Ref ID J:123000]

Additional References

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Price and Supply Information

Strain Name:	B6;129P2-Adam2tm1Dgm/J
Stock Number:	008042

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 29-SEP-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development

Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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