Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1F2+pN1 Generation Definitions   Donating Investigator Paul Primakoff,   University of California Davis

Description
Mice homozygous for this fertilin β (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin β proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin β (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).

Development
A targeting vector was designed to replace exon 14 of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd-derived BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6N to produce heterozygous mutant mice. The donating investigators maintained these mice by breeding homozygous females to heterozygous males for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Control Information

	Control
	Wild-type from the colony
	100492 B6129PF1/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Adam2^tm1Dgm/Adam2^tm1Dgm

        involves: 129P2/OlaHsd * C57BL/6N

• reproductive system phenotype
• abnormal fertilization
  • the ability of sperm from mutant mice to bind to and fuse with the egg plasma membrane is reduced   (MGI Ref ID J:50040)
  • once fused, egg activation proceeds normally   (MGI Ref ID J:50040)
  • sperm are unable to adhere to the zona pellucida   (MGI Ref ID J:50040)
• asthenozoospermia
  • migration of sperm from the uterus into the oviduct is impaired   (MGI Ref ID J:50040)
  • sperm count and motility are otherwise normal   (MGI Ref ID J:50040)
• reduced male fertility
  • fertility rate is 2% compared to wild-type   (MGI Ref ID J:50040)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Developmental Defects Affecting Gonads
      males only
Fertility Defects
      males only

Research Tools
Reproductive Biology Research
      male germ cells

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Adam2tm1Dgm
Allele Name		targeted mutation 1, Diana G Myles
Allele Type		Targeted (knock-out)
Common Name(s)		fertilin beta-;
Mutation Made By		Diana Myles,   University of California Davis
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14TG2a
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Adam2, a disintegrin and metallopeptidase domain 2
Chromosome		14
Gene Common Name(s)		AI323749; CRYN1; CRYN2; CT15; FTNB; Ftnb; PH-30; PH-30b; PH30; Ph30-beta; expressed sequence AI323749; fertilin beta;
Molecular Note		A neomycin resistance cassette replaced a genomic fragment containing exon 14, encoding the predicted disintegrin domain active site. Western blot analysis did not detect the encoded protein in spermatogenic cells and sperm derived from homozygous mice. [MGI Ref ID J:50040]

Genotyping

Genotyping Information

Genotyping Protocols

Adam2^tm1Dgm, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cho C; Bunch DO; Faure JE; Goulding EH; Eddy EM; Primakoff P; Myles DG. 1998. Fertilization defects in sperm from mice lacking fertilin beta. Science 281(5384):1857-9. [PubMed: 9743500]  [MGI Ref ID J:50040]

Nishimura H; Myles DG; Primakoff P. 2007. Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm. J Biol Chem 282(24):17900-7. [PubMed: 17439939]  [MGI Ref ID J:123000]

Additional References

Adam2^tm1Dgm related

Cho C; Ge H; Branciforte D; Primakoff P; Myles DG. 2000. Analysis of mouse fertilin in wild-type and fertilin beta(-/-) sperm: evidence for C-terminal modification, alpha/beta dimerization, and lack of essential role of fertilin alpha in sperm-egg fusion. Dev Biol 222(2):289-95. [PubMed: 10837118]  [MGI Ref ID J:62917]

Han C; Choi E; Park I; Lee B; Jin S; Kim do H; Nishimura H; Cho C. 2009. Comprehensive analysis of reproductive ADAMs: relationship of ADAM4 and ADAM6 with an ADAM complex required for fertilization in mice. Biol Reprod 80(5):1001-8. [PubMed: 19129510]  [MGI Ref ID J:149644]

Kim T; Oh J; Woo JM; Choi E; Im SH; Yoo YJ; Kim do H; Nishimura H; Cho C. 2006. Expression and Relationship of Male Reproductive ADAMs in Mouse. Biol Reprod 74(4):744-50. [PubMed: 16407499]  [MGI Ref ID J:107008]

Murase S; Cho C; White JM; Horwitz AF. 2008. ADAM2 promotes migration of neuroblasts in the rostral migratory stream to the olfactory bulb. Eur J Neurosci 27(7):1585-95. [PubMed: 18380661]  [MGI Ref ID J:133203]

Nishimura H; Cho C; Branciforte DR; Myles DG; Primakoff P. 2001. Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta. Dev Biol 233(1):204-13. [PubMed: 11319869]  [MGI Ref ID J:69282]

Stein KK; Go JC; Primakoff P; Myles DG. 2005. Defects in secretory pathway trafficking during sperm development in Adam2 knockout mice. Biol Reprod 73(5):1032-8. [PubMed: 16014818]  [MGI Ref ID J:115442]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous females are bred with heterozygous males. Homozygous males have severe sperm defects rendering them infertile.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	100492 B6129PF1/J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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