Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Heterozygote         (Female x Male) Species laboratory mouse   Donating Investigator Paul Primakoff,   University of California Davis

Description
Mice homozygous for this cyritestin (Adam3) mutant allele are viable with no gross phenotypic abnormalities reported. No protein product is detected from the targeted gene in sperm isolated from homozygous males. While homozygous females are fertile, homozygous males exhibit sperm deficiencies in adhesion to the egg zona pellucida and to the egg plasma membrane rendering them infertile. These cyritestin (Adam3) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the fertilin b (Adam2)-deficient strain (see Stock No. 008042).

Development
A targeting vector was designed to replace exon 14 of the targeted gene with a neomycin resistance cassette. The construct was electroporated into 129P2/OlaHsd-derived BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6N to produce heterozygous mutant mice. The donating investigators maintained these mice by breeding homozygous females to heterozygous males for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Control Information

	Control
	100492 B6129PF1/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Adam3^tm1Pmkf/Adam3^tm1Pmkf

        Background Not Specified

• reproductive system phenotype
• impaired fertilization (MGI Ref ID J:69282)
  • inability of the mutant sperm to bind to the zona pellucida
• male infertility (MGI Ref ID J:69282)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)

Research Tools
Reproductive Biology Research (male germ cells)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Adam3tm1Pmkf
Allele Name		targeted mutation 1, Paul Primakoff
Allele Type		Targeted (knock-out)
Common Name(s)		Cyrn -; cyritestin -;
Mutation Made By		Paul Primakoff,   University of California Davis
Gene Symbol and Name		Adam3, a disintegrin and metallopeptidase domain 3 (cyritestin)
Chromosome		8
Gene Common Name(s)		Cyrn1; Taz83; cyritestin 1; tMDC; tMDCI;
Molecular Note		A genomic fragment containing exon 14 was replaced with a neomycin selection cassette. Western blots on sperm extracts confirmed the absence of the full length protein in homozygous mice. [MGI Ref ID J:69282]

Genotyping

Genotyping Information

Genotyping Protocols

Adam3^tm1Pmkf, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Nishimura H; Cho C; Branciforte DR; Myles DG; Primakoff P. 2001. Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta. Dev Biol 233(1):204-13. [PubMed: 11319869]  [MGI Ref ID J:69282]

Nishimura H; Myles DG; Primakoff P. 2007. Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm. J Biol Chem 282(24):17900-7. [PubMed: 17439939]  [MGI Ref ID J:123000]

Additional References

Adam3^tm1Pmkf related

Kim T; Oh J; Woo JM; Choi E; Im SH; Yoo YJ; Kim do H; Nishimura H; Cho C. 2006. Expression and Relationship of Male Reproductive ADAMs in Mouse. Biol Reprod 74(4):744-50. [PubMed: 16407499]  [MGI Ref ID J:107008]

Stein KK; Go JC; Primakoff P; Myles DG. 2005. Defects in secretory pathway trafficking during sperm development in Adam2 knockout mice. Biol Reprod 73(5):1032-8. [PubMed: 16014818]  [MGI Ref ID J:115442]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous females are bred with heterozygous males. Homozygous males have severe sperm defects rendering them infertile.
Mating System	Homozygote x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	100492 B6129PF1/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.