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| Stock No.'s 008053, 008054, 008055, 008056, 008057, 008058, 008059 and 008060, are viable, fertile and diabetes resistant. These NOD congenic strains are the result of crossing segments of C57L/J derived Chromosome 11 into the NOD/Bdc background. These strains are useful for identifying candidate genes within the Idd4 subloci. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain C57L/J H2 Haplotype g7 Generation N5F42+F1 (30-JAN-08) Donating Investigator Marcia McDuffie, U. of Virginia School of Medicine Appearance
albino
Related Genotype: A/A Tyrc/TyrcDescription
This NOD congenic strain, commonly referred to as NOD.Lc11 or DR3, carries Chromosome 11 alleles, D11Mit314 (51Mb) through D11Mit42 (112Mb), derived from C57L/J including Idd4 and a candidate gene Csf2. Diabetes incidence in this diabetes resistant strain is reported to be 9% in the females and 0% in males compared to 70% in female and 40% in male NOD/Bdc control mice. NOD.Lc11 mice develop mild periductular infiltrates early in life and by 44 weeks of age histological analysis indicates healthy islet beta cells and normal amounts of stored insulin. Neonates injected with T cells from 8-week-old prediabetic NOD females and from old NOD mice were resistant to diabetes by 25 weeks of age. Splenic and lymph node derived T cells from NOD.Lc11 mice fail to cause diabetes or beta cell damage in NOD.scid neonatal mice, however 6 weeks post transfer the recipients develop severe invasive insulitis. Adult NOD.scid females that received T cells from NOD.Lc11 mice developed diabetes starting at 49 days post transfer (McDuffie, 2000; Semiromi et al 2007).There appear to be several diabetes resistant regions (Idd4 ) on Chromosome 11, therefore this strain will be useful to positionally clone the Idd4 resistant loci.
Development
This NOD congenic stock contains a large genomic segment from C57L/J introgressed into Chr 11. (C57L/J X NOD) F2 females homozygous for NOD derived H2g7, MHC class I K and MHC class II- I AB alleles were selected for backcrossing to NOD/Bdc. Breeders for further backcross to NOD/Bdc were selected based only on the dominant trait of lack of diabetes in females at 30 weeks of age. Diabetes resistant lines were analyzed using microsatellite markers. Microsatellite marker analysis identified one of the lines, DR4, with a large segment of C57L/J on Chr 7 (Stock No. 008061 NOD.L-(D7Mit253-D7Mit242)/McdfJ; while this line, DR3, (Stock No. 008053 NOD.L-(D11Mit314-D11Mit42)/McdfJ was identified with a large segment of C57L/J on Chr 11 (McDuffie, 2000). The C57L/J congenic interval retained in this stock includes Idd4 and candidate genes Csf2, Stat3, Stat5a and Stat5b alleles. In 2008, the Type 1 Diabetes Resource received this stock at N5F42.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Genes & Alleles
Gene & Allele Information
| Gene Symbol and Name | D11Mit42, DNA segment, Chr 11, Massachusetts Institute of Technology 42 | ||
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| Chromosome | 11 | ||
| Gene Symbol and Name | D11Mit314, DNA Segment, Chr 11, Massachusetts Institute of Technology 314 | ||
| Chromosome | 11 | ||
| Gene Symbol and Name | Idd4, insulin dependent diabetes susceptibility 4 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | Idd-4; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Davoodi-Semiromi A; McDuffie M; Litherland S; Clare-Salzler M. 2007. Truncated pStat5B is associated with the Idd4 locus in NOD mice. Biochem Biophys Res Commun 356(3):655-61. [PubMed: 17382905] [MGI Ref ID J:121519]
Laloraya M; Davoodi-Semiromi A; Kumar GP; McDuffie M; She JX. 2006. Impaired Crkl expression contributes to the defective DNA binding of Stat5b in nonobese diabetic mice. Diabetes 55(3):734-41. [PubMed: 16505237] [MGI Ref ID J:106850]
McDuffie M. 2000. Derivation of diabetes-resistant congenic lines from the nonobese diabetic mouse. Clin Immunol 96(2):119-30. [PubMed: 10900159] [MGI Ref ID J:63736]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $54.00 Female or Male Homozygous *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $108.00 Homozygous x Homozygous
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $70.20 Female or Male Homozygous *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $140.40 Homozygous x Homozygous
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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