Strain Name: |
NOD.L-(D11Mit314-D11Mit42)/McdfJ |
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Stock Number: |
008053 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | DR3; NOD.Lc11; |
| Genes & Alleles | D11Mit314; D11Mit42; Idd4; ; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain C57L/J Donating Investigator Marcia McDuffie, U. of Virginia School of Medicine H2 Haplotype g7 Generation N5F42+F1 (30-JAN-08) Appearance
albino
Related Genotype: A/A Tyrc/TyrcStrain Description
This NOD congenic strain, commonly referred to as NOD.Lc11 or DR3, carries Chromosome 11 alleles, D11Mit314 (51Mb) through D11Mit42 (112Mb), derived from C57L/J including Idd4 and a candidate gene Csf2. Diabetes incidence in this diabetes resistant strain is reported to be 9% in the females and 0% in males compared to 70% in female and 40% in male NOD/Bdc control mice. NOD.Lc11 mice develop mild periductular infiltrates early in life and by 44 weeks of age histological analysis indicates healthy islet beta cells and normal amounts of stored insulin. Neonates injected with T cells from 8-week-old prediabetic NOD females and from old NOD mice were resistant to diabetes by 25 weeks of age. Splenic and lymph node derived T cells from NOD.Lc11 mice fail to cause diabetes or beta cell damage in NOD.scid neonatal mice, however 6 weeks post transfer the recipients develop severe invasive insulitis. Adult NOD.scid females that received T cells from NOD.Lc11 mice developed diabetes starting at 49 days post transfer (McDuffie, 2000; Semiromi et al 2007).There appear to be several diabetes resistant regions (Idd4 ) on Chromosome 11, therefore this strain will be useful to positionally clone the Idd4 resistant loci.
Strain Development
This NOD congenic stock contains a large genomic segment from C57L/J introgressed into Chr 11. (C57L/J X NOD) F2 females homozygous for NOD derived H2g7, MHC class I K and MHC class II- I AB alleles were selected for backcrossing to NOD/Bdc. Breeders for further backcross to NOD/Bdc were selected based only on the dominant trait of lack of diabetes in females at 30 weeks of age. Diabetes resistant lines were analyzed using microsatellite markers. Microsatellite marker analysis identified one of the lines, DR4, with a large segment of C57L/J on Chr 7 (Stock No. 008061 NOD.L-(D7Mit253-D7Mit242)/McdfJ; while this line, DR3, (Stock No. 008053 NOD.L-(D11Mit314-D11Mit42)/McdfJ was identified with a large segment of C57L/J on Chr 11 (McDuffie, 2000). The C57L/J congenic interval retained in this stock includes Idd4 and candidate genes Csf2, Stat3, Stat5a and Stat5b alleles. In 2008, the Type 1 Diabetes Resource received this stock at N5F42.
| Gene Symbol and Name | D11Mit42, DNA segment, Chr 11, Massachusetts Institute of Technology 42 | ||
|---|---|---|---|
| Chromosome | 11 | ||
| Gene Symbol and Name | D11Mit314, DNA Segment, Chr 11, Massachusetts Institute of Technology 314 | ||
| Chromosome | 11 | ||
| Gene Symbol and Name | Idd4, insulin dependent diabetes susceptibility 4 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | Idd-4; | ||
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Diet Information | LabDiet® 5K52/5K67 |
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Congenic Nomenclature
Room Number AX11
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Selected Reference(s)
Davoodi-Semiromi A; McDuffie M; Litherland S; Clare-Salzler M. 2007. Truncated pStat5B is associated with the Idd4 locus in NOD mice. Biochem Biophys Res Commun 356(3):655-61. [PubMed: 17382905] [MGI Ref ID J:121519]
Laloraya M; Davoodi-Semiromi A; Kumar GP; McDuffie M; She JX. 2006. Impaired Crkl expression contributes to the defective DNA binding of Stat5b in nonobese diabetic mice. Diabetes 55(3):734-41. [PubMed: 16505237] [MGI Ref ID J:106850]
McDuffie M. 2000. Derivation of diabetes-resistant congenic lines from the nonobese diabetic mouse. Clin Immunol 96(2):119-30. [PubMed: 10900159] [MGI Ref ID J:63736]
| Strain Name: | NOD.L-(D11Mit314-D11Mit42)/McdfJ |
| Stock Number: | 008053 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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