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Strain Common Names	DR4;      NOD.Lc7;
Genes & Alleles	D7Mit242;   D7Mit253;   ;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain C57L/J Donating Investigator Marcia McDuffie,   U. of Virginia School of Medicine H2 Haplotype g7 Generation N4F44+F1 (30-JAN-08)

Appearance
albino
Related Genotype: A/A Tyr^c/Tyr^c

Strain Description
This NOD congenic strain, commonly referred to as NOD.Lc7 or DR4, carries Chr. 7 alleles, D7Mit253 (115Mb) through D7Mit242 (138Mb), derived from C57L/J and is distal to either Idd7 or Idd27. Diabetes incidence in this diabetes resistant strain is reported to be 10% in females and 0% in males compared to 70% in female and 40% in male NOD/Bdc controls. Both males and females develop widespread islet associated infiltrates and invasive insulitis, although the beta cell mass and insulin content are well maintained. NOD.Lc7 neonates injected with T-cells from old NOD mice were resistant to diabetes, however 100% of the female and 38% of the male NOD recipients receiving T cells from non diabetic, 5+ month old NOD.Lc7 mice developed diabetes starting at 12 weeks of age.

This strain is useful for identifying candidate genes within this new and unique Idd region and to eventually positionally clone the newly identified resistance loci.

Strain Development
This NOD congenic stock contains a genomic segment from C57L/J introgressed into Chr 7. (C57L/J X NOD) F2 females homozygous for NOD derived H2^g7, MHC class I K and MHC class II- I AB alleles were selected for backcrossing to NOD/Bdc. Breeders for further backcross to NOD/Bdc were selected based only on the dominant trait of lack of diabetes in females at 30 weeks of age. Diabetes resistant lines were analyzed using microsatellite markers. Microsatellite marker analysis identified one of the lines, DR3, (Stock No. 008053 NOD.L-(D11Mit314-D11Mit42)/McdfJ) with a large C57L/J segment on Chr 11; while this diabetes resistant line, DR4 (Stock No. 008061 NOD.L-(D7Mit253-D7Mit242)/McdfJ), was identified with a C57L/J segment (115- 138Mb) on Chr 7 (McDuffie, 2000). In 2008, the Type 1 Diabetes Resource received this stock at N4F44.

Gene & Allele Details

Gene Symbol and Name		D7Mit242, DNA segment, Chr 7, Massachusetts Institute of Technology 242
Chromosome		7
Gene Symbol and Name		D7Mit253, DNA segment, Chr 7, Massachusetts Institute of Technology 253
Chromosome		7

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)

References

Selected Reference(s)

Ling X; Cota-Gomez A; Flores NC; Hernandez-Saavedra D; McCord JM; Marecki JC; Haskins K; McDuffie M; Powers K; Kench J; Oka M; McMurtry I; Flores SC. 2005. Alterations in redox homeostasis and prostaglandins impair endothelial-dependent vasodilation in euglycemic autoimmune nonobese diabetic mice. Free Radic Biol Med 39(8):1089-98. [PubMed: 16198235]  [MGI Ref ID J:102117]

McDuffie M. 2000. Derivation of diabetes-resistant congenic lines from the nonobese diabetic mouse. Clin Immunol 96(2):119-30. [PubMed: 10900159]  [MGI Ref ID J:63736]

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Price and Supply Information

Strain Name:	NOD.L-(D7Mit253-D7Mit242)/McdfJ
Stock Number:	008061

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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