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| These Itgax-cre transgenic mice express Cre recombinase the control of the mouse integrin alpha X (or Cd11c) promoter. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study dendritic cell homeostasis and function. | |||||||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 11-MAR-08 Species laboratory mouse Generation N12+N3F2 (29-DEC-08) Donating Investigator Boris Reizis, Columbia University Medical Center Description
Mice hemizygous for the Cd11c-cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%), and myeloid cells (<1%). No increase of recombination frequency was observed in CD11clow- activated T cells. These Cd11c-cre transgenic mice (as well as CD11c-Cre-GFP transgenic mice (see Stock No. 007567)) are an effective tool for generating tissue-specific targeted mutants for studying dendritic cell homeostasis and function.Development
The expression cassette encoding Cre recombinase and a polyA signal sequence was introduced into the first exon of the mouse integrin alpha X (Cd11c) gene (Itgax), within the bacterial artificial chromosome (BAC) clone RP24-361C4. The resulting ~160 Kb transgenic construct was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. The mice were crossed to 129S6/SvEvTac for several generations and then backcrossed to C57BL/6 for more than 12 generations prior to arriving at The Jackson Laboratory
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Itgax
View Strains carrying other alleles of Itgax (5 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Itgax-cre)1-1Reiz | ||
|---|---|---|---|
| Allele Name | transgene insertion 1-1, Boris Reizis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Cd11c-cre; | ||
| Mutation Made By | Boris Reizis, Columbia University Medical Center | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | most conventional CD11chigh dendritic cells and many plasmacytoid dendritic cells; low expression in lymphocytes, NK cells, and myeloid cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Itgax, integrin alpha X, mouse, laboratory | ||
| Driver Note | Itgax | ||
| Molecular Note | A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter, and a bovine growth hormone poly A signal sequence, was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. Background recombination is detected in lymphocytes (<10%) and myeloid cells (<1%). [MGI Ref ID J:125869] | ||
| Gene Symbol and Name | Tg(Itgax-cre)1-1Reiz, transgene insertion 1-1, Boris Reizis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Cd11c-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Itgax-cre)1-1Reiz, Melt Curve Analysis
Tg(Itgax-cre)1-1Reiz, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Caton ML; Smith-Raska MR; Reizis B. 2007. Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen. J Exp Med 204(7):1653-64. [PubMed: 17591855] [MGI Ref ID J:125869]
Additional References
Tg(Itgax-cre)1-1Reiz relatedBirnberg T; Bar-On L; Sapoznikov A; Caton ML; Cervantes-Barragan L; Makia D; Krauthgamer R; Brenner O; Ludewig B; Brockschnieder D; Riethmacher D; Reizis B; Jung S. 2008. Lack of conventional dendritic cells is compatible with normal development and T cell homeostasis, but causes myeloid proliferative syndrome. Immunity 29(6):986-97. [PubMed: 19062318] [MGI Ref ID J:142682]
Cervantes-Barragan L; Kalinke U; Zust R; Konig M; Reizis B; Lopez-Macias C; Thiel V; Ludewig B. 2009. Type I IFN-mediated protection of macrophages and dendritic cells secures control of murine coronavirus infection. J Immunol 182(2):1099-106. [PubMed: 19124753] [MGI Ref ID J:143484]
Cisse B; Caton ML; Lehner M; Maeda T; Scheu S; Locksley R; Holmberg D; Zweier C; den Hollander NS; Kant SG; Holter W; Rauch A; Zhuang Y; Reizis B. 2008. Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell development. Cell 135(1):37-48. [PubMed: 18854153] [MGI Ref ID J:147515]
Cucak H; Yrlid U; Reizis B; Kalinke U; Johansson-Lindbom B. 2009. Type I interferon signaling in dendritic cells stimulates the development of lymph-node-resident T follicular helper cells. Immunity 31(3):491-501. [PubMed: 19733096] [MGI Ref ID J:152690]
Darrasse-Jeze G; Deroubaix S; Mouquet H; Victora GD; Eisenreich T; Yao KH; Masilamani RF; Dustin ML; Rudensky A; Liu K; Nussenzweig MC. 2009. Feedback control of regulatory T cell homeostasis by dendritic cells in vivo. J Exp Med 206(9):1853-62. [PubMed: 19667061] [MGI Ref ID J:152171]
Dietrich P; Shanmugasundaram R; Shuyu E; Dragatsis I. 2009. Congenital hydrocephalus associated with abnormal subcommissural organ in mice lacking huntingtin in Wnt1 cell lineages. Hum Mol Genet 18(1):142-50. [PubMed: 18838463] [MGI Ref ID J:143295]
Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82. [PubMed: 18656388] [MGI Ref ID J:139001]
Ohnmacht C; Pullner A; King SB; Drexler I; Meier S; Brocker T; Voehringer D. 2009. Constitutive ablation of dendritic cells breaks self-tolerance of CD4 T cells and results in spontaneous fatal autoimmunity. J Exp Med 206(3):549-59. [PubMed: 19237601] [MGI Ref ID J:146781]
Varol C; Vallon-Eberhard A; Elinav E; Aychek T; Shapira Y; Luche H; Fehling HJ; Hardt WD; Shakhar G; Jung S. 2009. Intestinal lamina propria dendritic cell subsets have different origin and functions. Immunity 31(3):502-12. [PubMed: 19733097] [MGI Ref ID J:152689]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous. Mating System +/+ sibling x Hemizygote (Female x Male) 11-MAR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Itgax-cre)1-1Reiz
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Itgax-cre)1-1Reiz x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Itgax-cre)1-1Reiz
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Itgax-cre)1-1Reiz
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Itgax-cre)1-1Reiz x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Itgax-cre)1-1Reiz
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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