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| These Itgax-cre transgenic mice express Cre recombinase the control of the mouse integrin alpha X (or Cd11c) promoter. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study dendritic cell homeostasis and function. | |||||||||||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 11-MAR-08 Species laboratory mouse Generation N12+N3F13 (30-JUN-11)
Generation DefinitionsDonating Investigator Boris Reizis, Columbia University Medical Center Description
Mice hemizygous for the Cd11c-cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%), and myeloid cells (<1%). No increase of recombination frequency was observed in CD11clow- activated T cells. These Cd11c-cre transgenic mice (as well as CD11c-Cre-GFP transgenic mice (see Stock No. 007567)) are an effective tool for generating tissue-specific targeted mutants for studying dendritic cell homeostasis and function.View cre expression characterization.
Development
The expression cassette encoding Cre recombinase and a polyA signal sequence was introduced into the first exon of the mouse integrin alpha X (Cd11c) gene (Itgax), within the bacterial artificial chromosome (BAC) clone RP24-361C4. The resulting ~160 Kb transgenic construct was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. The mice were crossed to 129S6/SvEvTac for several generations and then backcrossed to C57BL/6 for more than 12 generations prior to arriving at The Jackson Laboratory.SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (2 of 5 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Itgax
008378 B6.Cg-Tg(Itgax-TGFBR2)1Flv/J 008829 B6.Cg-Tg(Itgax-Venus)1Mnz/J 004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J 008830 C.Cg-Tg(Itgax-Venus)1Mnz/J 004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J 007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J 013233 NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J 009422 NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ 008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ View Strains carrying other alleles of Itgax (9 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Itgax-cre)1-1Reiz | ||
|---|---|---|---|
| Allele Name | transgene insertion 1-1, Boris Reizis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | CD11cCreT; Cd11c-cre; | ||
| Mutation Made By | Boris Reizis, Columbia University Medical Center | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | most conventional CD11chigh dendritic cells and many plasmacytoid dendritic cells; low expression in lymphocytes, NK cells, and myeloid cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Itgax, integrin alpha X, mouse, laboratory | ||
| Driver Note | Itgax | ||
| Molecular Note | A transgenic construct containing sequence encoding cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter, and a bovine growth hormone poly A signal sequence, was introduced into B6CBAF1/J donor eggs. BAC clone RP24-361C4 (BACPAC Resources) containing the Itgax (Cd11c) gene but lacking the 5' end of the adjacent Itgam gene, was modified by ET recombination to generated the transgene construct. Founder line 1-1 was consequently established. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. Background recombination is detected in lymphocytes (<10%) and myeloid cells (<1%). [MGI Ref ID J:125869] | ||
| Gene Symbol and Name | Tg(Itgax-cre)1-1Reiz, transgene insertion 1-1, Boris Reizis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Cd11c-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Itgax-cre)1-1Reiz, Melt Curve Analysis
Tg(Itgax-cre)1-1Reiz, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Caton ML; Smith-Raska MR; Reizis B. 2007. Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen. J Exp Med 204(7):1653-64. [PubMed: 17591855] [MGI Ref ID J:125869]
Additional References
Tg(Itgax-cre)1-1Reiz relatedBar-On L; Birnberg T; Kim KW; Jung S. 2011. Dendritic cell-restricted CD80/86 deficiency results in peripheral regulatory T-cell reduction but is not associated with lymphocyte hyperactivation. Eur J Immunol 41(2):291-8. [PubMed: 21267999] [MGI Ref ID J:175433]
Baschant U; Frappart L; Rauchhaus U; Bruns L; Reichardt HM; Kamradt T; Brauer R; Tuckermann JP. 2011. Glucocorticoid therapy of antigen-induced arthritis depends on the dimerized glucocorticoid receptor in T cells. Proc Natl Acad Sci U S A 108(48):19317-22. [PubMed: 22084093] [MGI Ref ID J:180395]
Bell BD; Kitajima M; Larson RP; Stoklasek TA; Dang K; Sakamoto K; Wagner KU; Reizis B; Hennighausen L; Ziegler SF. 2013. The transcription factor STAT5 is critical in dendritic cells for the development of TH2 but not TH1 responses. Nat Immunol 14(4):364-71. [PubMed: 23435120] [MGI Ref ID J:194747]
Birnberg T; Bar-On L; Sapoznikov A; Caton ML; Cervantes-Barragan L; Makia D; Krauthgamer R; Brenner O; Ludewig B; Brockschnieder D; Riethmacher D; Reizis B; Jung S. 2008. Lack of conventional dendritic cells is compatible with normal development and T cell homeostasis, but causes myeloid proliferative syndrome. Immunity 29(6):986-97. [PubMed: 19062318] [MGI Ref ID J:142682]
Cervantes-Barragan L; Kalinke U; Zust R; Konig M; Reizis B; Lopez-Macias C; Thiel V; Ludewig B. 2009. Type I IFN-mediated protection of macrophages and dendritic cells secures control of murine coronavirus infection. J Immunol 182(2):1099-106. [PubMed: 19124753] [MGI Ref ID J:143484]
Cervantes-Barragan L; Lewis KL; Firner S; Thiel V; Hugues S; Reith W; Ludewig B; Reizis B. 2012. Plasmacytoid dendritic cells control T-cell response to chronic viral infection. Proc Natl Acad Sci U S A 109(8):3012-7. [PubMed: 22315415] [MGI Ref ID J:182008]
Chen M; Felix K; Wang J. 2012. Critical role for perforin and Fas-dependent killing of dendritic cells in the control of inflammation. Blood 119(1):127-36. [PubMed: 22042696] [MGI Ref ID J:181665]
Cisse B; Caton ML; Lehner M; Maeda T; Scheu S; Locksley R; Holmberg D; Zweier C; den Hollander NS; Kant SG; Holter W; Rauch A; Zhuang Y; Reizis B. 2008. Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell development. Cell 135(1):37-48. [PubMed: 18854153] [MGI Ref ID J:147515]
Colonna L; Catalano G; Chew C; D'Agati V; Thomas JW; Wong FS; Schmitz J; Masuda ES; Reizis B; Tarakhovsky A; Clynes R. 2010. Therapeutic targeting of Syk in autoimmune diabetes. J Immunol 185(3):1532-43. [PubMed: 20601600] [MGI Ref ID J:162249]
Cucak H; Yrlid U; Reizis B; Kalinke U; Johansson-Lindbom B. 2009. Type I interferon signaling in dendritic cells stimulates the development of lymph-node-resident T follicular helper cells. Immunity 31(3):491-501. [PubMed: 19733096] [MGI Ref ID J:152690]
Dann A; Poeck H; Croxford AL; Gaupp S; Kierdorf K; Knust M; Pfeifer D; Maihoefer C; Endres S; Kalinke U; Meuth SG; Wiendl H; Knobeloch KP; Akira S; Waisman A; Hartmann G; Prinz M. 2012. Cytosolic RIG-I-like helicases act as negative regulators of sterile inflammation in the CNS. Nat Neurosci 15(1):98-106. [PubMed: 22138643] [MGI Ref ID J:180319]
Darrasse-Jeze G; Deroubaix S; Mouquet H; Victora GD; Eisenreich T; Yao KH; Masilamani RF; Dustin ML; Rudensky A; Liu K; Nussenzweig MC. 2009. Feedback control of regulatory T cell homeostasis by dendritic cells in vivo. J Exp Med 206(9):1853-62. [PubMed: 19667061] [MGI Ref ID J:152171]
Diao J; Mikhailova A; Tang M; Gu H; Zhao J; Cattral MS. 2012. Immunostimulatory conventional dendritic cells evolve into regulatory macrophage-like cells. Blood 119(21):4919-27. [PubMed: 22490680] [MGI Ref ID J:185155]
Dietrich P; Shanmugasundaram R; Shuyu E; Dragatsis I. 2009. Congenital hydrocephalus associated with abnormal subcommissural organ in mice lacking huntingtin in Wnt1 cell lineages. Hum Mol Genet 18(1):142-50. [PubMed: 18838463] [MGI Ref ID J:143295]
Drexler SK; Bonsignore L; Masin M; Tardivel A; Jackstadt R; Hermeking H; Schneider P; Gross O; Tschopp J; Yazdi AS. 2012. Tissue-specific opposing functions of the inflammasome adaptor ASC in the regulation of epithelial skin carcinogenesis. Proc Natl Acad Sci U S A 109(45):18384-9. [PubMed: 23090995] [MGI Ref ID J:191239]
Elliott ER; Van Ziffle JA; Scapini P; Sullivan BM; Locksley RM; Lowell CA. 2011. Deletion of Syk in neutrophils prevents immune complex arthritis. J Immunol 187(8):4319-30. [PubMed: 21918195] [MGI Ref ID J:179306]
Finney BA; Schweighoffer E; Navarro-Nunez L; Benezech C; Barone F; Hughes CE; Langan SA; Lowe KL; Pollitt AY; Mourao-Sa D; Sheardown S; Nash GB; Smithers N; Reis e Sousa C; Tybulewicz VL; Watson SP. 2012. CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development. Blood 119(7):1747-56. [PubMed: 22186994] [MGI Ref ID J:181715]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Ghosh HS; Cisse B; Bunin A; Lewis KL; Reizis B. 2010. Continuous expression of the transcription factor e2-2 maintains the cell fate of mature plasmacytoid dendritic cells. Immunity 33(6):905-16. [PubMed: 21145760] [MGI Ref ID J:167292]
Greenblatt MB; Aliprantis A; Hu B; Glimcher LH. 2010. Calcineurin regulates innate antifungal immunity in neutrophils. J Exp Med 207(5):923-31. [PubMed: 20421389] [MGI Ref ID J:160937]
Hammer GE; Turer EE; Taylor KE; Fang CJ; Advincula R; Oshima S; Barrera J; Huang EJ; Hou B; Malynn BA; Reizis B; DeFranco A; Criswell LA; Nakamura MC; Ma A. 2011. Expression of A20 by dendritic cells preserves immune homeostasis and prevents colitis and spondyloarthritis. Nat Immunol 12(12):1184-93. [PubMed: 22019834] [MGI Ref ID J:178962]
Hinterberger M; Aichinger M; da Costa OP; Voehringer D; Hoffmann R; Klein L. 2010. Autonomous role of medullary thymic epithelial cells in central CD4(+) T cell tolerance. Nat Immunol 11(6):512-9. [PubMed: 20431619] [MGI Ref ID J:160615]
Hofmann J; Mair F; Greter M; Schmidt-Supprian M; Becher B. 2011. NIK signaling in dendritic cells but not in T cells is required for the development of effector T cells and cell-mediated immune responses. J Exp Med 208(9):1917-29. [PubMed: 21807870] [MGI Ref ID J:177597]
Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82. [PubMed: 18656388] [MGI Ref ID J:139001]
Hou B; Saudan P; Ott G; Wheeler ML; Ji M; Kuzmich L; Lee LM; Coffman RL; Bachmann MF; Defranco AL. 2011. Selective Utilization of Toll-like Receptor and MyD88 Signaling in B Cells for Enhancement of the Antiviral Germinal Center Response. Immunity 34(3):375-84. [PubMed: 21353603] [MGI Ref ID J:170704]
Huang G; Wang Y; Vogel P; Kanneganti TD; Otsu K; Chi H. 2012. Signaling via the kinase p38alpha programs dendritic cells to drive TH17 differentiation and autoimmune inflammation. Nat Immunol 13(2):152-61. [PubMed: 22231518] [MGI Ref ID J:181211]
Iborra S; Izquierdo HM; Martinez-Lopez M; Blanco-Menendez N; Reis e Sousa C; Sancho D. 2012. The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice. J Clin Invest 122(5):1628-43. [PubMed: 22505455] [MGI Ref ID J:184532]
Ishifune C; Maekawa Y; Nishida J; Kitamura A; Tanigaki K; Yagita H; Yasutomo K. 2011. Notch signaling regulates the development of a novel type of Thy1-expressing dendritic cell in the thymus. Eur J Immunol 41(5):1309-20. [PubMed: 21469122] [MGI Ref ID J:175405]
Kaneko T; Saito Y; Kotani T; Okazawa H; Iwamura H; Sato-Hashimoto M; Kanazawa Y; Takahashi S; Hiromura K; Kusakari S; Kaneko Y; Murata Y; Ohnishi H; Nojima Y; Takagishi K; Matozaki T. 2012. Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiation and induces autoimmunity. J Immunol 188(11):5397-407. [PubMed: 22539788] [MGI Ref ID J:188740]
Kang TB; Yang SH; Toth B; Kovalenko A; Wallach D. 2013. Caspase-8 Blocks Kinase RIPK3-Mediated Activation of the NLRP3 Inflammasome. Immunity 38(1):27-40. [PubMed: 23260196] [MGI Ref ID J:192616]
Kel JM; Girard-Madoux MJ; Reizis B; Clausen BE. 2010. TGF-beta is required to maintain the pool of immature Langerhans cells in the epidermis. J Immunol 185(6):3248-55. [PubMed: 20713882] [MGI Ref ID J:163820]
Kellersch B; Brocker T. 2013. Langerhans cell homeostasis in mice is dependent on mTORC1 but not mTORC2 function. Blood 121(2):298-307. [PubMed: 23212520] [MGI Ref ID J:194626]
Kernbauer E; Maier V; Stoiber D; Strobl B; Schneckenleithner C; Sexl V; Reichart U; Reizis B; Kalinke U; Jamieson A; Muller M; Decker T. 2012. Conditional Stat1 ablation reveals the importance of interferon signaling for immunity to Listeria monocytogenes infection. PLoS Pathog 8(6):e1002763. [PubMed: 22719255] [MGI Ref ID J:195380]
Kim SJ; Zou YR; Goldstein J; Reizis B; Diamond B. 2011. Tolerogenic function of Blimp-1 in dendritic cells. J Exp Med 208(11):2193-9. [PubMed: 21948081] [MGI Ref ID J:178876]
Kirkland D; Benson A; Mirpuri J; Pifer R; Hou B; Defranco AL; Yarovinsky F. 2012. B Cell-Intrinsic MyD88 Signaling Prevents the Lethal Dissemination of Commensal Bacteria during Colonic Damage. Immunity 36(2):228-38. [PubMed: 22306056] [MGI Ref ID J:181331]
Kiss EA; Vonarbourg C; Kopfmann S; Hobeika E; Finke D; Esser C; Diefenbach A. 2011. Natural aryl hydrocarbon receptor ligands control organogenesis of intestinal lymphoid follicles. Science 334(6062):1561-5. [PubMed: 22033518] [MGI Ref ID J:179017]
Kohler T; Reizis B; Johnson RS; Weighardt H; Forster I. 2012. Influence of hypoxia-inducible factor 1alpha on dendritic cell differentiation and migration. Eur J Immunol 42(5):1226-36. [PubMed: 22539295] [MGI Ref ID J:187773]
Kool M; van Loo G; Waelput W; De Prijck S; Muskens F; Sze M; van Praet J; Branco-Madeira F; Janssens S; Reizis B; Elewaut D; Beyaert R; Hammad H; Lambrecht BN. 2011. The ubiquitin-editing protein a20 prevents dendritic cell activation, recognition of apoptotic cells, and systemic autoimmunity. Immunity 35(1):82-96. [PubMed: 21723156] [MGI Ref ID J:174380]
Kuipers H; Schnorfeil FM; Fehling HJ; Bartels H; Brocker T. 2010. Dicer-dependent microRNAs control maturation, function, and maintenance of Langerhans cells in vivo. J Immunol 185(1):400-9. [PubMed: 20530258] [MGI Ref ID J:161607]
Legutko A; Marichal T; Fievez L; Bedoret D; Mayer A; de Vries H; Klotz L; Drion PV; Heirman C; Cataldo D; Louis R; Thielemans K; Andris F; Leo O; Lekeux P; Desmet CJ; Bureau F. 2011. Sirtuin 1 promotes Th2 responses and airway allergy by repressing peroxisome proliferator-activated receptor-gamma activity in dendritic cells. J Immunol 187(9):4517-29. [PubMed: 21948987] [MGI Ref ID J:179447]
Lewis KL; Caton ML; Bogunovic M; Greter M; Grajkowska LT; Ng D; Klinakis A; Charo IF; Jung S; Gommerman JL; Ivanov II; Liu K; Merad M; Reizis B. 2011. Notch2 receptor signaling controls functional differentiation of dendritic cells in the spleen and intestine. Immunity 35(5):780-91. [PubMed: 22018469] [MGI Ref ID J:178710]
Luckashenak N; Wahe A; Breit K; Brakebusch C; Brocker T. 2013. Rho-Family GTPase Cdc42 Controls Migration of Langerhans Cells In Vivo. J Immunol 190(1):27-35. [PubMed: 23209325] [MGI Ref ID J:190821]
Mancino A; Habbeddine M; Johnson E; Luron L; Bebien M; Memet S; Fong C; Bajenoff M; Wu X; Karin M; Caamano J; Chi H; Seed M; Lawrence T. 2013. I kappa B kinase alpha (IKKalpha) activity is required for functional maturation of dendritic cells and acquired immunity to infection. EMBO J 32(6):816-28. [PubMed: 23422957] [MGI Ref ID J:195312]
Melillo JA; Song L; Bhagat G; Blazquez AB; Plumlee CR; Lee C; Berin C; Reizis B; Schindler C. 2010. Dendritic cell (DC)-specific targeting reveals Stat3 as a negative regulator of DC function. J Immunol 184(5):2638-45. [PubMed: 20124100] [MGI Ref ID J:159650]
Melton AC; Bailey-Bucktrout SL; Travis MA; Fife BT; Bluestone JA; Sheppard D. 2010. Expression of alphavbeta8 integrin on dendritic cells regulates Th17 cell development and experimental autoimmune encephalomyelitis in mice. J Clin Invest 120(12):4436-44. [PubMed: 21099117] [MGI Ref ID J:171861]
Mortier E; Advincula R; Kim L; Chmura S; Barrera J; Reizis B; Malynn BA; Ma A. 2009. Macrophage- and dendritic-cell-derived interleukin-15 receptor alpha supports homeostasis of distinct CD8+ T cell subsets. Immunity 31(5):811-22. [PubMed: 19913445] [MGI Ref ID J:155298]
Ng CT; Oldstone MB. 2012. Infected CD8alpha- dendritic cells are the predominant source of IL-10 during establishment of persistent viral infection. Proc Natl Acad Sci U S A 109(35):14116-21. [PubMed: 22893686] [MGI Ref ID J:189808]
Ochi A; Nguyen AH; Bedrosian AS; Mushlin HM; Zarbakhsh S; Barilla R; Zambirinis CP; Fallon NC; Rehman A; Pylayeva-Gupta Y; Badar S; Hajdu CH; Frey AB; Bar-Sagi D; Miller G. 2012. MyD88 inhibition amplifies dendritic cell capacity to promote pancreatic carcinogenesis via Th2 cells. J Exp Med 209(9):1671-87. [PubMed: 22908323] [MGI Ref ID J:191814]
Ohnmacht C; Pullner A; King SB; Drexler I; Meier S; Brocker T; Voehringer D. 2009. Constitutive ablation of dendritic cells breaks self-tolerance of CD4 T cells and results in spontaneous fatal autoimmunity. J Exp Med 206(3):549-59. [PubMed: 19237601] [MGI Ref ID J:146781]
Ohnmacht C; Schwartz C; Panzer M; Schiedewitz I; Naumann R; Voehringer D. 2010. Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity 33(3):364-74. [PubMed: 20817571] [MGI Ref ID J:164432]
Ohtani M; Hoshii T; Fujii H; Koyasu S; Hirao A; Matsuda S. 2012. Cutting edge: mTORC1 in intestinal CD11c+ CD11b+ dendritic cells regulates intestinal homeostasis by promoting IL-10 production. J Immunol 188(10):4736-40. [PubMed: 22504639] [MGI Ref ID J:188676]
Raetz M; Hwang SH; Wilhelm CL; Kirkland D; Benson A; Sturge CR; Mirpuri J; Vaishnava S; Hou B; Defranco AL; Gilpin CJ; Hooper LV; Yarovinsky F. 2013. Parasite-induced T(H)1 cells and intestinal dysbiosis cooperate in IFN-gamma-dependent elimination of Paneth cells. Nat Immunol 14(2):136-42. [PubMed: 23263554] [MGI Ref ID J:192614]
Ramalingam R; Larmonier CB; Thurston RD; Midura-Kiela MT; Zheng SG; Ghishan FK; Kiela PR. 2012. Dendritic cell-specific disruption of TGF-beta receptor II leads to altered regulatory T cell phenotype and spontaneous multiorgan autoimmunity. J Immunol 189(8):3878-93. [PubMed: 22972928] [MGI Ref ID J:190515]
Ritprajak P; Hayakawa M; Sano Y; Otsu K; Park JM. 2012. Cell type-specific targeting dissociates the therapeutic from the adverse effects of protein kinase inhibition in allergic skin disease. Proc Natl Acad Sci U S A 109(23):9089-94. [PubMed: 22615377] [MGI Ref ID J:184745]
Sathaliyawala T; O'Gorman WE; Greter M; Bogunovic M; Konjufca V; Hou ZE; Nolan GP; Miller MJ; Merad M; Reizis B. 2010. Mammalian target of rapamycin controls dendritic cell development downstream of flt3 ligand signaling. Immunity 33(4):597-606. [PubMed: 20933441] [MGI Ref ID J:165522]
Schwandt T; Schumak B; Gielen GH; Jungerkes F; Schmidbauer P; Klocke K; Staratschek-Jox A; van Rooijen N; Kraal G; Ludwig-Portugall I; Franken L; Wehner S; Kalff JC; Weber O; Kirschning C; Coch C; Kalinke U; Wenzel J; Kurts C; Zawatzky R; Holzmann B; Layland L; Schultze JL; Burgdorf S; den Haan JM; Knolle PA; Limmer A. 2012. Expression of type I interferon by splenic macrophages suppresses adaptive immunity during sepsis. EMBO J 31(1):201-13. [PubMed: 22036947] [MGI Ref ID J:180238]
Seillet C; Jackson JT; Markey KA; Brady HJ; Hill GR; Macdonald KP; Nutt SL; Belz GT. 2013. CD8alpha+ DCs can be induced in the absence of transcription factors Id2, Nfil3, and Batf3. Blood 121(9):1574-83. [PubMed: 23297132] [MGI Ref ID J:194760]
Solodova E; Jablonska J; Weiss S; Lienenklaus S. 2011. Production of IFN-beta during Listeria monocytogenes infection is restricted to monocyte/macrophage lineage. PLoS One 6(4):e18543. [PubMed: 21494554] [MGI Ref ID J:172226]
Subramanian M; Thorp E; Hansson GK; Tabas I. 2013. Treg-mediated suppression of atherosclerosis requires MYD88 signaling in DCs. J Clin Invest 123(1):179-88. [PubMed: 23257360] [MGI Ref ID J:194509]
Teichmann LL; Ols ML; Kashgarian M; Reizis B; Kaplan DH; Shlomchik MJ. 2010. Dendritic cells in lupus are not required for activation of T and B cells but promote their expansion, resulting in tissue damage. Immunity 33(6):967-78. [PubMed: 21167752] [MGI Ref ID J:167722]
Travis MA; Reizis B; Melton AC; Masteller E; Tang Q; Proctor JM; Wang Y; Bernstein X; Huang X; Reichardt LF; Bluestone JA; Sheppard D. 2007. Loss of integrin alpha(v)beta8 on dendritic cells causes autoimmunity and colitis in mice. Nature 449(7160):361-5. [PubMed: 17694047] [MGI Ref ID J:126778]
Varol C; Vallon-Eberhard A; Elinav E; Aychek T; Shapira Y; Luche H; Fehling HJ; Hardt WD; Shakhar G; Jung S. 2009. Intestinal lamina propria dendritic cell subsets have different origin and functions. Immunity 31(3):502-12. [PubMed: 19733097] [MGI Ref ID J:152689]
Wan CK; Oh J; Li P; West EE; Wong EA; Andraski AB; Spolski R; Yu ZX; He J; Kelsall BL; Leonard WJ. 2013. The Cytokines IL-21 and GM-CSF Have Opposing Regulatory Roles in the Apoptosis of Conventional Dendritic Cells. Immunity 38(3):514-27. [PubMed: 23453633] [MGI Ref ID J:194831]
Wang X; Li H; Matte-Martone C; Cui W; Li N; Tan HS; Roopenian D; Shlomchik WD. 2011. Mechanisms of antigen presentation to T cells in murine graft-versus-host disease: cross-presentation and the appearance of cross-presentation. Blood 118(24):6426-37. [PubMed: 21963602] [MGI Ref ID J:179099]
Wang Y; Huang G; Vogel P; Neale G; Reizis B; Chi H. 2012. Transforming growth factor beta-activated kinase 1 (TAK1)-dependent checkpoint in the survival of dendritic cells promotes immune homeostasis and function. Proc Natl Acad Sci U S A 109(6):E343-52. [PubMed: 22308391] [MGI Ref ID J:182619]
Wendland M; Willenzon S; Kocks J; Davalos-Misslitz AC; Hammerschmidt SI; Schumann K; Kremmer E; Sixt M; Hoffmeyer A; Pabst O; Forster R. 2011. Lymph node T cell homeostasis relies on steady state homing of dendritic cells. Immunity 35(6):945-57. [PubMed: 22195748] [MGI Ref ID J:179277]
Xie P; Poovassery J; Stunz LL; Smith SM; Schultz ML; Carlin LE; Bishop GA. 2011. Enhanced Toll-like receptor (TLR) responses of TNFR-associated factor 3 (TRAF3)-deficient B lymphocytes. J Leukoc Biol 90(6):1149-57. [PubMed: 21971520] [MGI Ref ID J:178855]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous. Mating System +/+ sibling x Hemizygote (Female x Male) 11-MAR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Itgax-cre)1-1Reiz
Price per Pair (US dollars $) Pair Genotype $302.00 Hemizygous for Tg(Itgax-cre)1-1Reiz x Noncarrier $302.00 Noncarrier x Hemizygous for Tg(Itgax-cre)1-1Reiz Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Itgax-cre)1-1Reiz
Price per Pair (US dollars $) Pair Genotype $392.60 Hemizygous for Tg(Itgax-cre)1-1Reiz x Noncarrier $392.60 Noncarrier x Hemizygous for Tg(Itgax-cre)1-1Reiz Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
|
|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
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Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.