Strain Name: |
B6.Cg-Tg(Itgax-cre)1-1Reiz/J |
|---|---|
Stock Number: |
008068 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Itgax; cre; Tg(Itgax-cre)1-1Reiz; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Boris Reizis, Columbia University Medical Center Generation N13+ (03-MAR-08) Strain Description
Mice hemizygous for the Cd11c-cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%), and myeloid cells (<1%). No increase of recombination frequency was observed in CD11clow- activated T cells. These Cd11c-cre transgenic mice (as well as CD11c-Cre-GFP transgenic mice (see Stock No. 007567)) are an effective tool for generating tissue-specific targeted mutants for studying dendritic cell homeostasis and function.Strain Development
The expression cassette encoding Cre recombinase and a polyA signal sequence was introduced into the first exon of the mouse integrin alpha X (Cd11c) gene (Itgax), within the bacterial artificial chromosome (BAC) clone RP24-361C4. The resulting ~160 Kb transgenic construct was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. The mice were crossed to 129S6/SvEvTac for several generations and then backcrossed to C57BL/6 for more than 12 generations prior to arriving at The Jackson Laboratory
Gene & Allele Details
| Allele Symbol | Tg(Itgax-cre)1-1Reiz | ||
|---|---|---|---|
| Allele Name | transgene insertion 1-1, Boris Reizis | ||
| Common Name(s) | Cd11c-cre; | ||
| Mutation Made By | Boris Reizis, Columbia University Medical Center | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | most conventional CD11chigh dendritic cells and many plasmacytoid dendritic cells; low expression in lymphocytes, NK cells, and myeloid cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Itgax, integrin alpha X, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter, and a bovine growth hormone poly A signal sequence, was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. Background recombination is detected in lymphocytes (<10%) and myeloid cells (<1%). [MGI Ref ID J:125869] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Tg(Itgax-cre)1-1Reiz
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Itgax
004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J 004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J 007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J 008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ View Strains carrying other alleles of Itgax (4 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Caton ML; Smith-Raska MR; Reizis B. 2007. Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen. J Exp Med 204(7):1653-64. [PubMed: 17591855] [MGI Ref ID J:125869]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Itgax-cre)1-1Reiz/J |
| Stock Number: | 008068 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Itgax-cre)1-1Reiz | |||
| Pair | $375.30 | Hemizygous for Tg(Itgax-cre)1-1Reiz x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Itgax-cre)1-1Reiz | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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