Strain Name:

B6;129P2-Pvalbtm1(cre)Arbr/J

Stock Number:

008069

Availability:

Under Development for Distribution Colony

Use Restrictions Apply, see Purchasing Information
To register your interest in this strain go to the Strain Interest Form.
This strain expresses Cre recombinase from the endogenous Pvalb, parvalbumin, locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. This mutant mouse strain represents a model that may be useful in studies of neuronal differentiation.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Specieslaboratory mouse
 
Donating Investigator Silvia Arber,   Friedrich Miescher Institute

Description
This strain expresses Cre recombinase from the endogenous Pvalb, parvalbumin, locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in more than 90% of neurons that express parvalbumin, such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia. This mutant mouse strain represents a model that may be useful in studies of neuronal differentiation.

Development
An IRES-Cre-pA targeting cassette containing cre coding sequence, internal ribosome entry site (IRES), and polyadenylation site sequence was inserted into the 3' UTR of exon 5. The endogenous Pvalb promoter drives expression of the Cre recombinase. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to produce homozygotes.

Additional Web Information

Cre-lox Systems

Phenotype

Phenotype Information

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)

Research Tools
Cre-lox System (Cre Recombinase Expression)
Neurobiology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol Pvalbtm1(cre)Arbr
Allele Name targeted mutation 1, Silvia Arber
Common Name(s) PVcre;
Mutation Made By Silvia Arber,   Friedrich Miescher Institute
Site of Expressionmost neurons that express parvalbumin including interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia
Gene Symbol and Name Pvalb, parvalbumin
Chromosome 15
Gene Common Name(s) D22S749; MGC116759; PALB1; PV; Pva;
Molecular Note An IRES-cre-pA cassette was inserted into the 3' UTR of exon 5. [MGI Ref ID J:100886]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, MCA, vers. 1
Generic Cre, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Hippenmeyer S; Vrieseling E; Sigrist M; Portmann T; Laengle C; Ladle DR; Arber S. 2005. A developmental switch in the response of DRG neurons to ETS transcription factor signaling. PLoS Biol 3(5):e159. [PubMed: 15836427]  [MGI Ref ID J:100886]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 15-DEC-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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