Strain Name: |
B6.129S4-Traf1tm1Tsi/J |
|---|---|
Stock Number: |
008076 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | C57BL/6 TRAF; |
| Genes & Alleles | Traf1; Traf1tm1Tsi; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Erdyni Tsitsikov, Immune Disease Institute (formerly CBRI) Generation N9+F1 (22-JUL-08) Strain Description
Mice homozygous for the TRAF1 mutant allele (TRAF1-/-) are viable and fertile. No protein expression from the targeted gene is observed in CD40-stimulated splenocytes isolated from homozygous mice. Homozygous mice on a C57BL/6 congenic background (B6-TRAF1-/-) have abnormal memory T cell survival and impaired influenza virus CD8 T cell responses. Activated B6-TRAF1-/- T cells accumulate increased levels of proapoptotic BH3-only family member Bim, particularly the most toxic isoform, Bims. The donating investigator reports that B6-TRAF1 mutant mice may be difficult to breed and gain more weight than BALB/c-TRAF1 mutant mice.Homozygous mice on a BALB/c congenic background (BALB/c-TRAF1-/-) exhibit acute liver injury and elevated serum liver enzymes following intratracheal TNF-alpha treatment. Furthermore, activated TRAF1-/- T cells have significantly increased expression of Th2 cytokines (IL-4, IL-5 and IL-13) that elicit enhanced Th2 responses in vivo. BALB/c-TRAF1-/- T cells exhibit elevated nuclear expression of NFAT-interacting protein (NIP45) and also induce significantly more intense pulmonary inflammation and higher airway hyper-responsiveness in OVA allergic inflammation models. Pulmonary leukocyte recruitment is attenuated following inhalation of lipopolysaccharide in BALB/c-TRAF1-/- mice. TRAF1 strains may be useful in studying negative regulation of tumor necrosis factor (TNF) signaling, NF-kB and AP-1 signaling, T cell receptor (TCR)-induced proliferation of T cells, Th2 responses, TRAF1/Bim function in CD8 memory T cell survival, allergic airway diseases and Rheumatoid arthritis, as well as the role of TRAF1 activation in the pathogenesis of lymphomas.
Of note, TRAF1 mutant mice are available on either a BALB/c (Stock No. 008074) or C57BL/6 (Stock No. 008076) congenic background.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector was designed to replace exons 2-5 (beginning at the first coding exon) with a neomycin resistance gene. This construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred with C57BL/6 females. Heterozygotes were then backcrossed to C57BL/6 inbred mice for at least 9 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Traf1tm1Tsi | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Erdyni Y Tsitsikov | ||
| Common Name(s) | TRAF1-; | ||
| Mutation Made By | Erdyni Tsitsikov, Immune Disease Institute (formerly CBRI) | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Traf1, Tnf receptor-associated factor 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | 4732496E14Rik; EBI6; MGC:10353; RIKEN cDNA 4732496E14 gene; | ||
| Molecular Note | The gene was disrupted by replacement of exons 2-5 with a neomycin resistance cassette. Homozygous mutant animals did not express protein product as determined by Western blot analysis of stimulated splenocytes using polyclonal antibodies directed against the C-terminus of the protein. [MGI Ref ID J:72327] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Traf1tm1Tsi
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice may be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Traf1tm1Tsi allele
008074 C.129S4-Traf1tm1Tsi/J View Strains carrying Traf1tm1Tsi (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Apoptosis Research
Endogenous Regulators
Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
Autoimmunity
Growth Factors/Receptors/Cytokines
Immunodeficiency (T cell deficiency)
Intracellular Signaling Molecules
Lymphoid Tissue Defects
T Cell Receptor Signaling Defects
Internal/Organ Research
Lymphoid Tissue Defects (T cell deficiency)
Research Tools
Apoptosis Research
Cancer Research (T cell deficiency)
Cancer Research (genes regulating lymphoma development)
Cancer Research (production of B and T cells, antibodies, and hybridomas)
Cancer Research (production of T cells and hybridoma)
Cell Biology Research
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Immunology and Inflammation Research (T Cell Receptor Deficiency)
Immunology and Inflammation Research (T cell deficiency)
Immunology and Inflammation Research (production of B cells, antibodies T cell lines, and hybridomas)
Immunology and Inflammation Research (production of T cell lines and hybridomas)
References
Selected Reference(s)
Additional ReferencesBryce PJ; Oyoshi MK; Kawamoto S; Oettgen HC; Tsitsikov EN. 2006. TRAF1 regulates Th2 differentiation, allergic inflammation and nuclear localization of the Th2 transcription factor, NIP45. Int Immunol 18(1):101-11. [PubMed: 16352630] [MGI Ref ID J:104165]
Oyoshi MK; Barthel R; Tsitsikov EN. 2007. TRAF1 regulates recruitment of lymphocytes and, to a lesser extent, neutrophils, myeloid dendritic cells and monocytes to the lung airways following lipopolysaccharide inhalation. Immunology 120(3):303-14. [PubMed: 17328785] [MGI Ref ID J:122710]
Pryhuber GS; Huyck HL; Roper JM; Cornejo J; O'reilly MA; Pierce RH; Tsitsikov EN. 2005. Acute Tumor Necrosis Factor-{alpha}-Induced Liver Injury in the Absence of Tumor Necrosis Factor Receptor-Associated Factor 1 Gene Expression. Am J Pathol 166(6):1637-45. [PubMed: 15920149] [MGI Ref ID J:98818]
Sabbagh L; Srokowski CC; Pulle G; Snell LM; Sedgmen BJ; Liu Y; Tsitsikov EN; Watts TH. 2006. A critical role for TNF receptor-associated factor 1 and Bim down-regulation in CD8 memory T cell survival. Proc Natl Acad Sci U S A 103(49):18703-8. [PubMed: 17116875] [MGI Ref ID J:118166]
Tsitsikov EN; Laouini D; Dunn IF; Sannikova TY; Davidson L; Alt FW; Geha RS. 2001. Traf1 is a negative regulator of tnf signaling. enhanced tnf signaling in traf1-deficient mice. Immunity 15(4):647-57. [PubMed: 11672546] [MGI Ref ID J:72327]
Zhang B; Wang Z; Li T; Tsitsikov EN; Ding HF. 2007. NF-kappaB2 mutation targets TRAF1 to induce lymphomagenesis. Blood 110(2):743-51. [PubMed: 17405906] [MGI Ref ID J:123872]
Price and Supply Information
| Strain Name: | B6.129S4-Traf1tm1Tsi/J |
| Stock Number: | 008076 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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