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| These BChE (butyrylcholinesterase) mutant mice are a model for human butyrylcholinesterase deficiency and may be useful for studying metabolic effects of organophosphorus toxicants or nerve agents, neurotransmitter function, and anti-Alzheimer's drug therapies. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N11+ (27-MAY-08) Donating Investigator Oksana Lockridge, Eppley Inst. (Univ of Nebraska Med Cntr) Description
Mice homozygous for this BChE mutant allele are viable and fertile with no reported spontaneous abnormalities. All tissues and plasma from homozygous mice are devoid of BChE activity. As BChE (butyrylcholinesterase) is a bioscavenger molecule protecting acetylcholinesterase (AChE) activity against nerve agents and organophosphates, homozygous BChE-deficiency leads to impaired protection from toxic compounds. These BChE mutant mice are a model for human butyrylcholinesterase deficiency and may be useful for studying metabolic effects of organophosphorus toxicants or nerve agents, neurotransmitter function, and anti-Alzheimer's drug therapies.Of note, the donating investigator has multiple strains available that may be useful for testing toxic compounds, including the AChE-deficient (Stock No. 005987), G117H BChE transgenic (Stock No. 007577), and BChE-deficient (see Stock No. 008077 and Stock No. 008087) mice.
Development
The targeting vector was designed to replace the sequence containing the splice junction between intron 1 and exon 2 through the entire signal peptide (including the translation start site and the first 102 amino acids of the encoded BChE protein) with a neomycin cassette. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ inbred mice. Mutant mice were backcrossed to 129S1/SvImJ for more than 10 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Bchetm1Loc allele
008087 B6.129S1-Bchetm1Loc/J View Strains carrying Bchetm1Loc (1 strain)
Phenotype
Phenotype Information
assigned by genotype
Bchetm1Loc/Bchetm1Loc
involves: 129S1/Sv
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:124975)
- mice are healthy and fertile, with no obvious abnormalities in appearance or behavior
This mouse can be used to support research in many areas including:
Cancer Research
Toxicology
Metabolism Research
Neurobiology Research
Neuromuscular Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects (cholinergic receptor)
Tremor Defects
Research Tools
Cancer Research
Metabolism Research
Neurobiology Research
Sensorineural Research
Toxicology Research (drug metabolism)
Toxicology Research (drug/compound testing)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Bchetm1Loc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Oksana Lockridge | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | BChE-; | ||
| Mutation Made By | Oksana Lockridge, Eppley Inst. (Univ of Nebraska Med Cntr) | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Gene Symbol and Name | Bche, butyrylcholinesterase | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | C730038G20Rik; CHE1; E1; RIKEN cDNA C730038G20 gene; | ||
| Molecular Note | 891bp of the gene is replaced with a neomycin selection cassette, including 485bp from the 3' end of intron 1 and 406bp from the 5' end of exon 2. The region encoding the entire signal peptide, the translation start site, and the first 102 amino acids ofthe mature protein is deleted. [MGI Ref ID J:124975] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Bchetm1Loc, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Duysen EG; Li B; Darvesh S; Lockridge O. 2007. Sensitivity of butyrylcholinesterase knockout mice to (--)-huperzine A and donepezil suggests humans with butyrylcholinesterase deficiency may not tolerate these Alzheimer's disease drugs and indicates butyrylcholinesterase function in neurotransmission. Toxicology 233(1-3):60-9. [PubMed: 17194517] [MGI Ref ID J:130048]
Li B; Duysen EG; Saunders TL; Lockridge O. 2006. Production of the butyrylcholinesterase knockout mouse. J Mol Neurosci 30(1-2):193-5. [PubMed: 17192674] [MGI Ref ID J:124975]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as heterozygotes or homozygotes. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 01-DEC-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Bchetm1Loc $291.90 Female or Male Homozygous for Bchetm1Loc *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Bchetm1Loc x Heterozygous for Bchetm1Loc
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Bchetm1Loc $379.50 Female or Male Homozygous for Bchetm1Loc *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Bchetm1Loc x Heterozygous for Bchetm1Loc
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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