Strain Name:

129S-Ppargtm2Yba/J

Stock Number:

008079

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Availability:

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These mice express tTA (tetracycline regulated transactivator) and a tetO-driven Flag-tagged Pparg from the endogenous Pparg locus. Although heterozygous mice exhibit lipodystrophy, hyperinsulinemia, pancreatic islet hyperplasia, and severe glucose intolerance, they do not develop steatosis or type II diabetes. Treatment with doxycycline prevents these phenotypes when administered starting at midgestation whereas a 6 week treatment administered to pubertal heterozygotes reverses most of these phenotypes. This strain may be useful in studies of lipodystrophy.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Yaacov Barak,   University of Pittsburgh

Description
These mice express tTA (tetracycline regulated transactivator) and a tetO-driven Flag-tagged Pparg from the endogenous Pparg locus. A fusion transcript (including the 5' portion of Pparg, IRES and tTA (AF1-IRES-tTA)), a Flag-Pparg transcript and a wildtype transcript are detected by Northem blot analysis of white adipose tissue from heterozygotes. The fusion transcript of AF1-IRES-tTA is also detected in brown adipose tissue. Expression of tTA is detected in adipose tissue by Western blot analysis. No Flag-PPARG1 protein was detected in adipose tissue. Weak expression of tTA and the Flag-Pparg transcript is observed in placenta and liver. Endogenous PPARG2 protein is reduced in adipose tissues of heterozygotes. Heterozygotes exhibit "buffalo humps" (swollen interscapular fat pads swollen by hypertrophy and unilocular lipid deposition in mutant brown adipocytes), absence of subcutaneous adipocytes, reduced gonadal white adipose tissue, irregularly residual adipocyte shape and size, and fibrotic white adipose tissue stroma with leukocytic infiltration of the adipose tissue. Although, mutant mice have hyperinsulinemia, pancreatic islet hyperplasia, severe glucose intolerance, and hepatomegaly, they do not develop steatosis or type II diabetes. Prepubertal male heterozygotes exhibit hyperglycemia which normalizes with age. Treatment with doxycycline prevents this phenotype when administered starting at midgestation. A 6 week doxycycline treatment administered to pubertal heterozygotes reverses most of the phenotype, except the reduction in white adipose tissue. Mice that are homozygous for the targeted mutation are not viable. This mutant mouse strain may be useful in studies of lipodystrophy.

Development
The Ldi cassette, containing IRES sequence, tTA (tetracycline regulated transactivator), PGK-neo, polyA, and a flag-tagged Pparg (peroxisome proliferator activated receptor gamma) cDNA sequence, was used to replace exon 2. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ mice. The Donating Investigator maintained the strain as a heterozygote because of the homozygous lethal phenotype.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pparg
004584   B6.129-Ppargtm2Rev/J
006142   B6.129S4-Ppargtm1Rev/J
008227   B6.129S4-Ppargtm3Yba/J
View Strains carrying other alleles of Pparg     (3 strains)

Strains carrying other alleles of tTA
016198   129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ
011008   B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
009602   B6.129S4(Cg)-Kcnn2tm2Jpad/J
009603   B6.129S4-Kcnn3tm1Jpad/J
008227   B6.129S4-Ppargtm3Yba/J
012359   B6.Cg-Pvalbtm1.1(tTA2)Hze/J
016868   B6.Cg-Ssttm1.2(tTA2)Hze/J
007004   B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
003563   B6.Cg-Tg(Cebpb-tTA)5Bjd/J
003767   B6.Cg-Tg(Eno2tTA)5021Nes/J
003763   B6.Cg-Tg(Eno2tTA)5030Nes/J
018306   B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
005964   B6.Cg-Tg(GFAP-tTA)110Pop/J
002618   B6.Cg-Tg(MMTVtTA)1Mam/J
008284   B6.Cg-Tg(Scg2-tTA)1Jt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
017722   B6.Cg-Tg(Tal1-tTA)19Dgt/J
017754   B6;129-Omptm1(tTA)Gogo/J
007585   B6;129S4-Npytm2Rpa/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
003010   B6;CBA-Tg(Camk2a-tTA)1Mmay/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
010573   B6;SJL-Tg(Prl-tTA)6-5Jek/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
008603   C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
010712   C57BL/6-Tg(Camk2a-tTA)1Stl/J
013585   FVB-Tg(Cdh5-tTA)D5Lbjn/J
005625   FVB-Tg(Pcp2-tTA)3Horr/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
006209   FVB.Cg-Tg(Tal1-tTA)19Dgt/J
005942   FVB/N-Tg(Pf4-tTA/VP16)42Kra/J
004937   NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
008335   STOCK Foxa2tm1.1(rtTa)Moon/J
008600   STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
005701   STOCK Pdx1tm1Macd/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
003271   STOCK Tg(CMV-tTA)3Bjd/J
015838   STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-ABL1*P242E*P249E)CPdav/J
018124   STOCK Tg(Prnp-tTA)F959Sbp/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003275   STOCK Tg(tetL)1Bjd/J
003274   STOCK Tg(tetNZL)2Bjd/J
View Strains carrying other alleles of tTA     (43 strains)

Strains carrying other alleles of tetO
016176   B6(Cg)-Tg(tetO-Per2)2Jt/J
009602   B6.129S4(Cg)-Kcnn2tm2Jpad/J
009603   B6.129S4-Kcnn3tm1Jpad/J
017983   B6.Cg-Col1a1tm9(tetO-Dnmt3b*)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
014588   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1A1tm6(tetO-MSI2)Jae/J
014648   B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
016998   B6.Cg-Tg(TetO-Axin1,EGFP)TA6Cos/J
003762   B6.Cg-Tg(tetFosb)4468Nes/J
007051   B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax
007052   B6.Cg-Tg(tetO-APPSwInd)107Dbo/Mmjax
007049   B6.Cg-Tg(tetO-APPSwInd)885Dbo/Mmjax
007618   B6.Cg-Tg(tetO-Arntl)1Jt/J
017555   B6.Cg-Tg(tetO-CALY)5Cber/J
008277   B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J
008468   B6.Cg-Tg(tetO-DTA)1Gfi/J
017791   B6.Cg-Tg(tetO-Hamp)2181Nca/J
009344   B6.Cg-Tg(tetO-Ifng)184Pop/J
009136   B6.Cg-Tg(tetO-Kcnj2,lacZ)1Gogo/J
013583   B6.Cg-Tg(tetO-LRRK2)C7874Cai/J
020652   B6.Cg-Tg(tetO-Mif)279Aren/J
017331   B6.Cg-Tg(tetO-Ppp3ca*)11255Kndl/J
017332   B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J
017330   B6.Cg-Tg(tetO-TAg*)175Kndl/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
011001   B6;129S4-Col1a1tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
016841   B6;C3-Tg(tetO-TARDBP)12Vle/J
014650   B6;C3-Tg(tetO-TARDBP*)4Vle/J
012450   B6;D2-Tg(tetO-SNCA)1Cai/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
010575   B6;SJL-Tg(tetO-Egfr*)2-9Jek/J
010577   B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J
002621   B6;SJL-Tg(tetop-lacZ)2Mam/J
006004   B6C3-Tg(tetO-APPSwInd)885Dbo/Mmjax
016976   B6C3-Tg(tetO-SNCA*A53T)33Vle/J
018913   B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
017719   C3HeB/FeJ-Tg(tetO-TAg)1Efr/J
017955   C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
017613   C57BL/6-Tg(tetO-Cdkn1b)1Scpr/J
013729   C57BL/6-Tg(tetO-EDN1,-lacZ)9Mhus/J
010713   C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J
013728   C57BL/6-Tg(tetO-NOS2,-lacZ)240iMhus/J
016181   C57BL/6-Tg(tetO-Nr1d1)1Schb/J
008278   C57BL/6J-Tg(tetO-Clock)1Jt/J
021065   FVB(C)-Tg(tetO-Npc1/YFP)1Mps/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
014153   FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J
014154   FVB-Tg(Myh6/tetO-Itpr2)4.9Jmol/J
012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
010580   FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J
013156   FVB-Tg(tetO-CDK5R1*)1Vln/J
013777   FVB-Tg(tetO-Cacna1g)1Jmol/J
013778   FVB-Tg(tetO-Cacnb2)1Jmol/J
013779   FVB-Tg(tetO-Cacnb2)2Jmol/J
013780   FVB-Tg(tetO-Cib1)1Jmol/J
010578   FVB-Tg(tetO-Dusp6)1Jmol/J
017333   FVB-Tg(tetO-Gnai2*,-lacZ)382Kndl/J
008685   FVB-Tg(tetO-Kdr*)4377.5Rwng/J
015815   FVB-Tg(tetO-MAPT*P301L)#Kha/JlwsJ
008695   FVB-Tg(tetO-MET)23Rwng/J
012387   FVB-Tg(tetO-Ppargc1a)1Dpk/J
012385   FVB-Tg(tetO-Ppargc1b)7Dpk/J
006439   FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
005941   FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
006202   FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J
014547   FVB/N-Tg(tetO-Fasl)BDepa/J
019376   FVB/N-Tg(tetO-MYC)36aBop/J
003315   FVB/N-Tg(tetORo1-lacZ)3Conk/J
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
011004   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
018999   STOCK Gt(ROSA)26Sortm1(tTA,tetO-Mir155)Fjsl/J
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Smn1tm1Msd Tg(SMN2)89Ahmb Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Smn1tm1Msd Tg(SMN2)89Ahmb Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
015838   STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-ABL1*P242E*P249E)CPdav/J
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
014544   STOCK Tg(tetO-ABL1*P242E*P249E)CPdav/J
014093   STOCK Tg(tetO-CHRM3*)1Blr/J
008790   STOCK Tg(tetO-DISC1*)1001Plet/J
008168   STOCK Tg(tetO-DTA)1Gfi/J
017755   STOCK Tg(tetO-GCAMP2)12iRyu/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
005728   STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
012441   STOCK Tg(tetO-LRRK2*G2019S)E3Cai/J
017599   STOCK Tg(tetO-SMN2,-luc)#aAhmb/J
017600   STOCK Tg(tetO-SMN2,-luc)#bAhmb/J
012442   STOCK Tg(tetO-SNCA*A53T)E2Cai/J
006224   STOCK Tg(tetO-cre)1Jaw/J
017906   STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
012449   STOCK Tg(teto-LRRK2)C7874Cai/J
View Strains carrying other alleles of tetO     (109 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Lipodystrophy, Familial Partial, Type 3; FPLD3
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Lipodystrophy, Congenital Generalized, Type 2; CGL2
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Carotid Intimal Medial Thickness 1   (PPARG)
Diabetes Mellitus, Noninsulin-Dependent; NIDDM   (PPARG)
Obesity   (PPARG)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ppargtm2Yba/Pparg+

        involves: 129S1/SvImJ * C57BL/6
  • reproductive system phenotype
  • reduced fertility
    • mice exhibit poor fecundity after introgression onto a C57BL/6 background   (MGI Ref ID J:125992)
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype
    • despite the development of dyslipidemia and severe insulin resistance, mice do not develop steatosis or type 2 diabetes   (MGI Ref ID J:125992)
    • abnormal brown adipose tissue morphology
      • mice exhibit hypertrophy and unilocular lipid deposition in mutant brown adipocytes   (MGI Ref ID J:125992)
      • however, defects can be prevented by midgestational administration of doxycycline and reverted by a 6 week treatment with doxycycline   (MGI Ref ID J:125992)
      • increased brown fat cell size
        • mice exhibit hypertrophy in mutant brown adipocytes   (MGI Ref ID J:125992)
        • however, defects can be prevented by midgestational administration of doxycycline and reverted by a 6 week treatment with doxycycline   (MGI Ref ID J:125992)
    • abnormal white adipose tissue morphology
      • fibrotic white adipose tissue stroma is filled with fragmented lipid droplets and leukocytes   (MGI Ref ID J:125992)
      • remaining adipocytes have irregular shapes and range in size from overtly hypertrophic to minuscule cells   (MGI Ref ID J:125992)
      • however, defects were prevented by midgestational administration of doxycycline but adipocyte size does not revert following treatment with doxycycline   (MGI Ref ID J:125992)
      • abnormal white fat cell morphology
        • remaining adipocytes have irregular shapes   (MGI Ref ID J:125992)
        • abnormal white fat cell size
          • remaining adipocytes range in size from overtly hypertrophic to minuscule cells   (MGI Ref ID J:125992)
      • absent subcutaneous adipose tissue
        • mice lack subcutaneous adipocytes   (MGI Ref ID J:125992)
    • decreased gonadal fat pad weight
      • gonadal fat pads are severely reduced compared to in wild-type mice   (MGI Ref ID J:125992)
      • however, defects can be prevented by midgestational administration of doxycycline and reverted by a 6 week treatment with doxycycline   (MGI Ref ID J:125992)
    • increased interscapular fat pad weight
      • mice exhibit 'buffalo humps' comprised of swollen interscapular fat pads due to hypertrophy and unilocular lipid deposition in mutant brown adipocytes   (MGI Ref ID J:125992)
      • however, defects can be prevented by midgestational administration of doxycycline and reverted by a 6 week treatment with doxycycline   (MGI Ref ID J:125992)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • despite the development of dyslipidemia and severe insulin resistance, mice do not develop steatosis or type 2 diabetes   (MGI Ref ID J:125992)
    • hyperglycemia
      • hyperglycemia develops prepuberty then normalizes latter in life with only sporadic and transient hyperglycemia   (MGI Ref ID J:125992)
      • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
    • impaired glucose tolerance
      • mice exhibit severe glucose intolerance   (MGI Ref ID J:125992)
      • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
    • increased circulating cholesterol level
      • mice exhibit an increase in serum cholesterol with early onset   (MGI Ref ID J:125992)
      • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
    • increased circulating free fatty acid level
      • mice exhibit an increase in serum free fatty acids with early onset   (MGI Ref ID J:125992)
      • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
    • increased circulating insulin level
      • mice exhibit an increase in serum insulin   (MGI Ref ID J:125992)
      • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
    • increased circulating triglyceride level
      • mice exhibit an increase in serum triglycerides with early onset   (MGI Ref ID J:125992)
      • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
    • insulin resistance   (MGI Ref ID J:125992)
  • endocrine/exocrine gland phenotype
  • pancreas hyperplasia
    • mice as old as 12 to 18 months exhibit hyperplasia with intact and insulin-laden pancreatic islets with hyperinsulinemia and normoglycemia   (MGI Ref ID J:125992)
  • pancreatic islet hyperplasia
    • mice exhibit pancreas islet cell hyperplasia   (MGI Ref ID J:125992)
    • however, treatment with doxycycline reverses metabolic anomalies   (MGI Ref ID J:125992)
  • liver/biliary system phenotype
  • abnormal hepatocyte morphology
    • mice exhibit hepatocyte hypertrophy and vacuolization   (MGI Ref ID J:125992)
  • enlarged liver
    • liver size is increased by 60% to 150% and is accompanied by hepatocyte hypertrophy and vacuolization   (MGI Ref ID J:125992)
    • however, lipid droplet accumulation is not observed   (MGI Ref ID J:125992)
  • integument phenotype
  • absent subcutaneous adipose tissue
    • mice lack subcutaneous adipocytes   (MGI Ref ID J:125992)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)

Metabolism Research
Lipid Metabolism

Research Tools
Metabolism Research
Tet Expression Systems
      tTA/rtTA Expressing Strains
      tTA/rtTA Responsive Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ppargtm2Yba
Allele Name targeted mutation 2, Yaacob Barak
Allele Type Targeted (knock-in)
Common Name(s) Ppargldi;
Mutation Made By Yaacov Barak,   University of Pittsburgh
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Site of ExpressionExpression of tTA from the endogenous Pparg locus is detected in adipose tissue.
Expressed Gene tetO, tet operator,
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Molecular Note Exon 2 was replaced with an Idi cassette that contains an IRES-tTA, neo, poly-adenylation signal, TetO and flag-tagged cDNA. While expression is modest in adipose tissue, it is poor in the liver and placenta. [MGI Ref ID J:125992]
 
 
Gene Symbol and Name Pparg, peroxisome proliferator activated receptor gamma
Chromosome 6
Gene Common Name(s) CIMT1; GLM1; NR1C3; PPAR-gamma; PPARG1; PPARG2; PPARgamma; PPARgamma2; Ppar-gamma2;

Genotyping

Genotyping Information

Genotyping Protocols

Ppargtm2Yba, Melt Curve Analysis
Ppargtm2Yba, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kim S; Huang LW; Snow KJ; Ablamunits V; Hasham MG; Young TH; Paulk AC; Richardson JE; Affourtit JP; Shalom-Barak T; Bult CJ; Barak Y. 2007. A mouse model of conditional lipodystrophy. Proc Natl Acad Sci U S A 104(42):16627-32. [PubMed: 17921248]  [MGI Ref ID J:125992]

Additional References

Ppargtm2Yba related

Arck P; Toth B; Pestka A; Jeschke U. 2010. Nuclear Receptors of the Peroxisome Proliferator-Activated Receptor (PPAR) Family in Gestational Diabetes: From Animal Models to Clinical Trials. Biol Reprod 83(2):168-76. [PubMed: 20427759]  [MGI Ref ID J:161967]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are not viable. The Donating Investigator maintained the strain by crossing heterozygous males, between 3 and 5 months of age, to wildtype 129S1/SvImJ females. This strain has a problematic fecundity with only about half of the heterozygous male animals between 3 and 5 months of age reproducing.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $1980.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2574.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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