Strain Name:

CBy.129(B6)-Sirt1tm1Ygu/J

Stock Number:

008081

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These SirT1co/co mice may be useful in generating conditional mutations for studying the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development and breast cancer.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN8F1pN1
Generation Definitions
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile with a loxP-flanked neomycin cassette just upstream of, and a third loxP site just downstream of exon 4 of the targeted gene. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding a conserved Sir2 motif) deleted in the cre-expressing tissue(s) (the donating investigator reports that they observe only one recombination event; complete removal of the neomycin cassette and exon 4, leaving a single loxp site remaining). These SirT1co/co mice may be useful in generating conditional mutations for studying the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, breast cancer, apoptosis, and metabolic diseases.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These SirT1co/co mice originated on a mixed genetic background (see Stock No. 008041). It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector was designed to insert a loxP-flanked, reverse-oriented neomycin cassette just upstream of, and a third loxP site just downstream of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the chimera's were bred with C57BL/6 mice. The resulting heterozygotes were interbred to generate homozygous SirT1co/co mice on this mixed B6;129 genetic background. Homozygotes were bred together for many generations prior to arrival at The Jackson Laboratory (as Stock No. 008041). Upon arrival, some mice were backcrossed to BALB/cByJ inbred mice (see Stock No. 001026) for at least 5 generations to generate this congenic strain (Stock No. 008081).

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Sirt1tm1Ygu allele
008041   B6;129-Sirt1tm1Ygu/J
View Strains carrying   Sirt1tm1Ygu     (1 strain)

Strains carrying other alleles of Sirt1
024510   B6.Cg-Tg(Sirt1)ASrn/J
013080   C57BL/6-Actbtm3.1(Sirt1)Npa/J
View Strains carrying other alleles of Sirt1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Sirt1tm1Ygu/Sirt1tm1Ygu

        involves: 129/Sv * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:121948)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines
Other

Cell Biology Research
Transcriptional Regulation

Diabetes and Obesity Research
Insulin Receptors and Growth Factors

Endocrine Deficiency Research
Mammary Gland Defects

Research Tools
Apoptosis Research
Cancer Research
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: transcriptional activation
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Sirt1tm1Ygu
Allele Name targeted mutation 1, Yansong Gu
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) SirT1co; Sirt1flox;
Mutation Made ByDr. Yansong Gu,   University of Washington School of Med.
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Sirt1, sirtuin 1
Chromosome 10
Gene Common Name(s) AA673258; SIR2L1; Sir2; Sir2alpha; expressed sequence AA673258;
Molecular Note A floxed neomycin cassette was inserted just upstream of exon 4 and a third loxP site was inserted just downstream. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. When bred tomice that express Cre recombinase, the resulting offspring will have exon 4 (encoding a conserved Sir2 motif) deleted in the cre-expressing tissue(s). [MGI Ref ID J:121948] [MGI Ref ID J:85542]

Genotyping

Genotyping Information

Genotyping Protocols

Sirt1tm1Ygu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Li H; Rajendran GK; Liu N; Ware C; Rubin BP; Gu Y. 2007. SirT1 modulates the estrogen-insulin-like growth factor-1 signaling for postnatal development of mammary gland in mice. Breast Cancer Res 9(1):R1. [PubMed: 17201918]  [MGI Ref ID J:121948]

Additional References

Sirt1tm1Ygu related

Chen D; Bruno J; Easlon E; Lin SJ; Cheng HL; Alt FW; Guarente L. 2008. Tissue-specific regulation of SIRT1 by calorie restriction. Genes Dev 22(13):1753-7. [PubMed: 18550784]  [MGI Ref ID J:137371]

Cheng HL; Mostoslavsky R; Saito S; Manis JP; Gu Y; Patel P; Bronson R; Appella E; Alt FW; Chua KF. 2003. Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice. Proc Natl Acad Sci U S A 100(19):10794-9. [PubMed: 12960381]  [MGI Ref ID J:85542]

Dietrich MO; Antunes C; Geliang G; Liu ZW; Borok E; Nie Y; Xu AW; Souza DO; Gao Q; Diano S; Gao XB; Horvath TL. 2010. Agrp neurons mediate Sirt1's action on the melanocortin system and energy balance: roles for Sirt1 in neuronal firing and synaptic plasticity. J Neurosci 30(35):11815-25. [PubMed: 20810901]  [MGI Ref ID J:164001]

Dietrich MO; Bober J; Ferreira JG; Tellez LA; Mineur YS; Souza DO; Gao XB; Picciotto MR; Araujo I; Liu ZW; Horvath TL. 2012. AgRP neurons regulate development of dopamine neuronal plasticity and nonfood-associated behaviors. Nat Neurosci 15(8):1108-10. [PubMed: 22729177]  [MGI Ref ID J:191603]

Herranz D; Serrano M. 2010. SIRT1: recent lessons from mouse models. Nat Rev Cancer 10(12):819-23. [PubMed: 21102633]  [MGI Ref ID J:166994]

Hsu CP; Zhai P; Yamamoto T; Maejima Y; Matsushima S; Hariharan N; Shao D; Takagi H; Oka S; Sadoshima J. 2010. Silent information regulator 1 protects the heart from ischemia/reperfusion. Circulation 122(21):2170-82. [PubMed: 21060073]  [MGI Ref ID J:179477]

Legutko A; Marichal T; Fievez L; Bedoret D; Mayer A; de Vries H; Klotz L; Drion PV; Heirman C; Cataldo D; Louis R; Thielemans K; Andris F; Leo O; Lekeux P; Desmet CJ; Bureau F. 2011. Sirtuin 1 promotes Th2 responses and airway allergy by repressing peroxisome proliferator-activated receptor-gamma activity in dendritic cells. J Immunol 187(9):4517-29. [PubMed: 21948987]  [MGI Ref ID J:179447]

Leko V; Park GJ; Lao U; Simon JA; Bedalov A. 2013. Enterocyte-specific inactivation of SIRT1 reduces tumor load in the APC(+/min) mouse model. PLoS One 8(6):e66283. [PubMed: 23799088]  [MGI Ref ID J:204211]

Liu Y; Dentin R; Chen D; Hedrick S; Ravnskjaer K; Schenk S; Milne J; Meyers DJ; Cole P; Yates J rd; Olefsky J; Guarente L; Montminy M. 2008. A fasting inducible switch modulates gluconeogenesis via activator/coactivator exchange. Nature 456(7219):269-73. [PubMed: 18849969]  [MGI Ref ID J:141418]

Menzies KJ; Singh K; Saleem A; Hood DA. 2013. Sirtuin 1-mediated effects of exercise and resveratrol on mitochondrial biogenesis. J Biol Chem 288(10):6968-79. [PubMed: 23329826]  [MGI Ref ID J:196710]

Oka S; Alcendor R; Zhai P; Park JY; Shao D; Cho J; Yamamoto T; Tian B; Sadoshima J. 2011. PPARalpha-Sirt1 complex mediates cardiac hypertrophy and failure through suppression of the ERR transcriptional pathway. Cell Metab 14(5):598-611. [PubMed: 22055503]  [MGI Ref ID J:179686]

Panossian L; Fenik P; Zhu Y; Zhan G; McBurney MW; Veasey S. 2011. SIRT1 Regulation of Wakefulness and Senescence-Like Phenotype in Wake Neurons. J Neurosci 31(11):4025-36. [PubMed: 21411645]  [MGI Ref ID J:170455]

Purushotham A; Schug TT; Xu Q; Surapureddi S; Guo X; Li X. 2009. Hepatocyte-specific deletion of SIRT1 alters fatty acid metabolism and results in hepatic steatosis and inflammation. Cell Metab 9(4):327-38. [PubMed: 19356714]  [MGI Ref ID J:148170]

Purushotham A; Xu Q; Lu J; Foley JF; Yan X; Kim DH; Kemper JK; Li X. 2012. Hepatic deletion of SIRT1 decreases hepatocyte nuclear factor 1alpha/farnesoid X receptor signaling and induces formation of cholesterol gallstones in mice. Mol Cell Biol 32(7):1226-36. [PubMed: 22290433]  [MGI Ref ID J:183692]

Schug TT; Xu Q; Gao H; Peres-da-Silva A; Draper DW; Fessler MB; Purushotham A; Li X. 2010. Myeloid deletion of SIRT1 induces inflammatory signaling in response to environmental stress. Mol Cell Biol 30(19):4712-21. [PubMed: 20647536]  [MGI Ref ID J:164917]

Vila-Brau A; De Sousa-Coelho AL; Mayordomo C; Haro D; Marrero PF. 2011. Human HMGCS2 Regulates Mitochondrial Fatty Acid Oxidation and FGF21 Expression in HepG2 Cell Line. J Biol Chem 286(23):20423-30. [PubMed: 21502324]  [MGI Ref ID J:173493]

Wauters E; Sanchez-Arevalo Lobo VJ; Pinho AV; Mawson A; Herranz D; Wu J; Cowley MJ; Colvin EK; Njicop EN; Sutherland RL; Liu T; Serrano M; Bouwens L; Real FX; Biankin AV; Rooman I. 2013. Sirtuin-1 regulates acinar-to-ductal metaplasia and supports cancer cell viability in pancreatic cancer. Cancer Res 73(7):2357-67. [PubMed: 23370328]  [MGI Ref ID J:196877]

Wen L; Chen Z; Zhang F; Cui X; Sun W; Geary GG; Wang Y; Johnson DA; Zhu Y; Chien S; Shyy JY. 2013. Ca2+/calmodulin-dependent protein kinase kinase beta phosphorylation of Sirtuin 1 in endothelium is atheroprotective. Proc Natl Acad Sci U S A 110(26):E2420-7. [PubMed: 23754392]  [MGI Ref ID J:197976]

Wu Z; Liu MC; Liang M; Fu J. 2012. Sirt1 protects against thrombomodulin down-regulation and lung coagulation after particulate matter exposure. Blood 119(10):2422-9. [PubMed: 22262770]  [MGI Ref ID J:182540]

Yang H; Lee SM; Gao B; Zhang J; Fang D. 2013. Histone deacetylase sirtuin 1 deacetylates IRF1 protein and programs dendritic cells to control Th17 protein differentiation during autoimmune inflammation. J Biol Chem 288(52):37256-66. [PubMed: 24214980]  [MGI Ref ID J:207189]

Yao H; Chung S; Hwang JW; Rajendrasozhan S; Sundar IK; Dean DA; McBurney MW; Guarente L; Gu W; Ronty M; Kinnula VL; Rahman I. 2012. SIRT1 protects against emphysema via FOXO3-mediated reduction of premature senescence in mice. J Clin Invest 122(6):2032-45. [PubMed: 22546858]  [MGI Ref ID J:188297]

Zhou X; Fan LX; Sweeney WE Jr; Denu JM; Avner ED; Li X. 2013. Sirtuin 1 inhibition delays cyst formation in autosomal-dominant polycystic kidney disease. J Clin Invest 123(7):3084-98. [PubMed: 23778143]  [MGI Ref ID J:201423]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryMutant mice were bred to BALB/cByJ inbred mice (see Stock No. 001026) for many generations to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)