Strain Name: |
B6CBA(FVB)-Tg(Prnp-TBP*)13Xjl/J |
|---|---|
Stock Number: |
008083 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Prnp; Tbp; Tg(Prnp-TBP*)13Xjl; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Xiao-Jiang Li, Emory University School of Medicine Generation N11+ (10-JUN-08) Strain Description
These transgenic mice express human TATA box binding protein, TBP, containing a 13 polyQ repeat expansion, under the control of the mouse prion protein promoter. Western blot analysis could not distinguish between endogenous and mutant TBP protein. Immunoreactive TBP protein was not detected in the brains of 4 month old mutants. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of spinocerebellar ataxia 17 (SCA17) and polyglutamine (polyQ) related neurodegeneration. These mice serve as a control strain for B6CBA(FVB)-Tg(Prnp-TBP*)105Xjl/J (Stock No. 008075) and B6CBA(FVB)-Tg(Prnp-TBP*)71-16Xjl/J (Stock No. 008216).Strain Development
A transgenic construct containing the human TATA box binding protein, TBP, containing a 13 polyQ repeat expansion, under the control of the mouse prion protein promoter was injected into fertilized FVB/N mouse eggs. Founder line TBP-13Q was established. Male animals were bred to FVB/NJ mice. The mice were then crossed to B6CBAF1/J (Stock No. 100011 ) mice for 10 generations.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Prnp-TBP*)13Xjl | ||
|---|---|---|---|
| Allele Name | transgene insertion 13, Xiao-Jiang Li | ||
| Common Name(s) | TBP-13Q; | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Tbp, TATA box binding protein, mouse, laboratory | ||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the human TATA box binding protein, TBP, containing a 13 polyQ repeat expansion, under the control of the mouse prion protein promoter was injected into fertilized FVB/N mouse eggs. Founder line TBP-13Q was established. Western blot analysis could not distinguish between endogenous and mutant TBP protein. Immunoreactive TBP protein was not detected in 4 month old brains. [MGI Ref ID J:130775] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100011 B6CBAF1/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not attempted to make the strain homozygous. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Prnp
View Strains carrying other alleles of Prnp (18 strains)
008075 B6CBA(FVB)-Tg(Prnp-TBP*)105Xjl/J 008216 B6CBA(FVB)-Tg(Prnp-TBP*)71-16Xjl/J
Animal Health Reports
Room Number AX11
References
Selected Reference(s)
Friedman MJ; Shah AG; Fang ZH; Ward EG; Warren ST; Li S; Li XJ. 2007. Polyglutamine domain modulates the TBP-TFIIB interaction: implications for its normal function and neurodegeneration. Nat Neurosci 10(12):1519-28. [PubMed: 17994014] [MGI Ref ID J:130775]
Price and Supply Information
| Strain Name: | B6CBA(FVB)-Tg(Prnp-TBP*)13Xjl/J |
| Stock Number: | 008083 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Prnp-TBP*)13Xjl | |||
| Pair | $375.30 | Hemizygous for Tg(Prnp-TBP*)13Xjl x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Prnp-TBP*)13Xjl | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form