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| When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 31-JUL-08 Species laboratory mouse Generation N5+N1F3 (18-NOV-09) Donating Investigator Eric Brown, Univ of Penn - Abramson Inst (AFCRI) Description
Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues.Development
This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Morula and blastocyst stage embryos were implanted into pseudopregnant recipient mice. A founder with a single-copy integrant expressing high levels of Cre-ERT2 was chosen to establish the Cre-ERT2 lentitransgenic mouse line. These mice were backcrossed to C57BL/6 mice for at least four generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(UBC-cre/ESR1)1Ejb allele
007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 007001 B6;129S-Tg(UBC-cre/ESR1)1Ejb/J View Strains carrying Tg(UBC-cre/ESR1)1Ejb (2 strains)
006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 005975 B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 008529 B6;129P-Tg(Neurog1-cre/ESR1)1Good/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 002712 FVB/N-Tg(PF4MER)6Kra/J 007684 STOCK Tg(Atoh1-cre/ESR1)14Fsh/J 008861 STOCK Tg(Ela1-Cre/ESR1)1Stof/J
006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 004353 C57BL/6-Tg(UBC-GFP)30Scha/J 007076 CByJ.B6-Tg(UBC-GFP)30Scha/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(UBC-cre/ESR1)1Ejb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Eric J Brown | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(UBC-cre)1Ejb; Ubc-cre; | ||
| Mutation Made By | Eric Brown, Univ of Penn - Abramson Inst (AFCRI) | ||
| Strain of Origin | (129S/SvEv x C57BL/6) | ||
| Site of Expression | tamoxifen-inducible cre activity in all tested tissues | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Promoter | UBC, ubiquitin C, human | ||
| Driver Note | UBC | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Mice display tamoxifen-inducible Cre activity in all tissue types. [MGI Ref ID J:123200] | ||
| Gene Symbol and Name | Tg(UBC-cre/ESR1)1Ejb, transgene insertion 1, Eric J Brown | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(UBC-cre)1Ejb; Ubc-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ruzankina Y; Pinzon-Guzman C; Asare A; Ong T; Pontano L; Cotsarelis G; Zediak VP; Velez M; Bhandoola A; Brown EJ. 2007. Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss. Cell Stem Cell 1(1):113-26. [PubMed: 18371340] [MGI Ref ID J:123200]
Additional References
Tg(UBC-cre/ESR1)1Ejb relatedGruber M; Hu CJ; Johnson RS; Brown EJ; Keith B; Simon MC. 2007. Acute postnatal ablation of Hif-2alpha results in anemia. Proc Natl Acad Sci U S A 104(7):2301-6. [PubMed: 17284606] [MGI Ref ID J:119731]
Lum JJ; Bui T; Gruber M; Gordan JD; Deberardinis RJ; Covello KL; Simon MC; Thompson CB. 2007. The transcription factor HIF-1{alpha} plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev 21(9):1037-49. [PubMed: 17437992] [MGI Ref ID J:121261]
Maillard I ; Chen YX ; Friedman A ; Yang Y ; Tubbs AT ; Shestova O ; Pear WS ; Hua X. 2009. Menin regulates the function of hematopoietic stem cells and lymphoid progenitors. Blood 113(8):1661-9. [PubMed: 19228930] [MGI Ref ID J:146598]
O'Driscoll M. 2009. Mouse models for ATR deficiency. DNA Repair (Amst) 8(11):1333-7. [PubMed: 19782648] [MGI Ref ID J:153964]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings. Attempts to breed hemi x hemi mice yielded no homozygous animals as determined by PCR (Jul-2008). Mating System Noncarrier x Hemizygote (Female x Male) 31-JUL-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(UBC-cre/ESR1)1Ejb x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(UBC-cre/ESR1)1Ejb x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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